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Method for preparing lysine-rich fermented soybean meal

A technology of fermented soybean meal and high lysine, applied in animal feed, animal feed, application, etc., can solve the problems of increased cost of adding lysine, high production cost of lysine, etc., to improve palatability and increase feed intake , Improve the effect of fermentation efficiency

Active Publication Date: 2012-11-07
广东希普生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Combining the two, for the advanced lysine production method, its production process has high requirements on equipment and strict process control, which leads to high production cost of lysine, which invisibly increases the cost of adding lysine to feed The problem

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] 1) Preparation of strains

[0034] a. Inoculate Brevibacterium flavum into the Brevibacterium flavum liquid culture medium, and culture it with shaking at 28-37°C. When the OD590 value of the bacterial solution is 0.25-0.30, stop the cultivation, and obtain the first-class strain of Brevibacterium flavum;

[0035] b. Inoculate the yeast into the yeast liquid culture medium, and culture it with shaking at 28-37°C. When the OD590 value of the bacteria liquid is 0.25-0.30, stop the culture, and obtain the first-class strain of yeast;

[0036] c. Inoculate the Lactobacillus into the Lactobacillus liquid culture medium, and culture it statically at 30-37°C. When the OD590 value of the bacterial solution is 0.25-0.30, stop the cultivation, and obtain the first-class strain of Lactobacillus;

[0037] in:

[0038] The concentration of each component in the Brevibacterium flavum culture medium is: peptone 8 g / L, yeast extract 9 g / L, sodium chloride 6 g / L, pH 7.0.

[0039] ...

example 2

[0047] 1) Preparation of strains

[0048] a. Inoculate Brevibacterium flavum into the Brevibacterium flavum liquid culture medium, and culture it with shaking at 28-37°C. When the OD590 value of the bacterial solution is 0.25-0.30, stop the cultivation, and obtain the first-class strain of Brevibacterium flavum;

[0049] b. Inoculate the yeast on the yeast culture medium, and culture it with shaking at 28-37°C. When the OD590 value of the bacterial solution is 0.25-0.30, stop the cultivation, and obtain the first-class strain of yeast;

[0050] c. Inoculate the Lactobacillus into the Lactobacillus liquid culture medium, and culture it statically at 30-37°C. When the OD590 value of the bacterial solution is 0.25-0.30, stop the cultivation, and obtain the first-class strain of Lactobacillus;

[0051] in:

[0052] The concentration of each component in the Brevibacterium flavum culture medium is: peptone 10 g / L, yeast extract 8 g / L, sodium chloride 5 g / L, pH 7.0.

[0053] T...

example 3

[0061] 1) Preparation of strains

[0062] a. Inoculate Brevibacterium flavum into the Brevibacterium flavum liquid culture medium, and culture it with shaking at 28-37°C. When the OD590 value of the bacterial solution is 0.25-0.30, stop the cultivation, and obtain the first-class strain of Brevibacterium flavum;

[0063] b. Inoculate the yeast into the yeast liquid culture medium, and culture it with shaking at 28-37°C. When the OD590 value of the bacteria liquid is 0.25-0.30, stop the culture, and obtain the first-class strain of yeast;

[0064] c. Inoculate the Lactobacillus into the Lactobacillus liquid culture medium, and culture it statically at 30-37°C. When the OD590 value of the bacterial solution is 0.25-0.30, stop the cultivation, and obtain the first-class strain of Lactobacillus;

[0065] The concentration of each component in the Brevibacterium flavum culture medium is: peptone 9 g / L, yeast extract 10 g / L, sodium chloride 4 g / L, pH 7.0.

[0066] The concentration...

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Abstract

The invention discloses a method for preparing lysine-rich fermented soybean meal, which is simple and can effectively improve the content of nutrients of the soybean meal. The method is technically characterized by comprising the following steps: (1) preparing strains: a, inoculating the brevibacterium flavum into the brevibacterium flavum liquid culture medium, and vibrating and culturing at 28-37 DEG C to obtain the first-level brevibacterium flavum strain when the OD590 value of the brevibacterium flavum liquid is 0.25-0.30; b, the inoculating the saccharomycetes into the saccharomycetes liquid culture medium, and vibrating and culturing at 28-37 DEG C to obtain the first-level saccharomycetes strain when the OD590 value of the saccharomycetes liquid is 0.25-0.30; and c, inoculating the lactobacillus into the lactobacillus liquid culture medium, and vibrating and culturing at 30-37 DEG C to obtain the first-level lactobacillus strain when the OD590 value of the lactobacillus liquid is 0.25-0.30; (2) preparing a fermentation substrate; and (3) inoculating the fermentation substrate. The method for preparing the lysine-rich fermented soybean meal belongs to the technical field of feed preparation.

Description

technical field [0001] The invention discloses a method for preparing fermented soybean meal, in particular to a method for preparing fermented soybean meal rich in lysine; it belongs to the technical field of feed preparation. Background technique [0002] Lysine is a basic amino acid, the second largest amino acid product next to glutamic acid, and the first limiting amino acid of cereal protein. Adding an appropriate amount of lysine to cereal foods will increase the biological value of protein. Greatly improve. [0003] Lysine has a wide range of applications: ① as a food fortifier; ② as a drug, it can be used as a liver cell regeneration agent, which has obvious curative effects on improving liver function, treating liver cirrhosis, hyperammonia, increasing appetite, and improving nutritional status; ③ As a feed additive, adding a small amount of lysine to the feed of livestock and poultry will have a particularly significant effect on the daily gain of poultry and liv...

Claims

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Application Information

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IPC IPC(8): A23K1/14A23K1/02A23K10/33
CPCY02P60/87
Inventor 王华荣恽辉余忠丽程林春赵大伟
Owner 广东希普生物科技股份有限公司
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