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Preparation method of L-tyrosine

A tyrosine and supercritical technology, which is applied in the field of L-tyrosine preparation, can solve the problems that the extraction method needs to be improved and the extraction effect needs to be improved, so as to achieve high extraction capacity and separation efficiency, improve stripping efficiency, and water-soluble good sex effect

Inactive Publication Date: 2016-03-30
NINGBO YUANFA BIOENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

For example, Liu Yaping et al. used the AOT reverse micelles system to extract and separate L-tyrosine in "Research on Separation of Tyrosine by Reverse Micromicelle Extraction" (Amino Acids and Biological Resources, 2001, 23(2): 32-35). The process conditions of extraction and back extraction are optimized, but the extraction method needs to be improved, and the extraction effect needs to be improved

Method used

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  • Preparation method of L-tyrosine

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Effect test

Embodiment 1

[0036] A preparation method of L-tyrosine, comprising the steps of:

[0037] Brevibacteria are inoculated on the medium, fermented and cultured to obtain a fermented liquid, the fermented liquid is centrifuged and ultrafiltered to obtain an ultrafiltrate, the ultrafiltration molecular weight cut-off is 1000-2000, the pH value of the ultrafiltrate is adjusted to 4.5, and NaCl is added to adjust The ionic strength is up to 0.30mol / L.

[0038] Add NaCl, buffer disodium hydrogen phosphate-citric acid, and 2.0% w / v porous silica gel to pure water to make a buffer solution with a pH value of 9.0 and an ionic strength of 1.4 mol / L, that is, back extraction water phase solution.

[0039] Add ultrafiltrate, surfactant and THF accounting for 13% v / v of surfactant into a supercritical extractor for supercritical extraction. The surfactant is a mixture of AOT and DTDPA with a molar ratio of 6:3. Surfactants and CO in supercritical extractors 2 Supercritical fluid forms supercritical fl...

Embodiment 2

[0042] A preparation method of L-tyrosine, comprising the steps of:

[0043] Inoculate the Brevibacterium on the culture medium, ferment and cultivate to obtain the fermentation liquid, centrifuge the fermentation liquid, and ultrafilter to obtain the ultrafiltrate, the ultrafiltration molecular weight cut-off is 1000-2000, adjust the pH value of the ultrafiltrate to 4.2, and add KCl to adjust The ionic strength is up to 0.23mol / L.

[0044] Prepare the stripping aqueous phase solution, add KCl, buffering agent citric acid-sodium citrate, and 0.5% w / v porous silica gel to pure water to make a buffer with a pH value of 8.5 and an ionic strength of 1.2 mol / L solution, that is, stripping the aqueous phase solution.

[0045] Add the ultrafiltrate, surfactant and THF accounting for 10% v / v of the surfactant into the supercritical extractor for supercritical extraction. The surfactant is a mixture of AOT and DTDPA with a molar ratio of 4:3. Surfactants and CO in supercritical extra...

Embodiment 3

[0048] A preparation method of L-tyrosine, comprising the steps of:

[0049] Brevibacteria are inoculated on the medium, fermented and cultivated to obtain a fermented liquid, the fermented liquid is centrifuged and ultrafiltered to obtain an ultrafiltrate, the ultrafiltration molecular weight cut-off is 1000-2000, the pH value of the ultrafiltrate is adjusted to 5.0, and NaCl is added to adjust The ionic strength is up to 0.35mol / L.

[0050] Prepare the back extraction aqueous phase solution, add KCl, buffer acetic acid-sodium acetate, and 4.0% w / v porous silica gel to pure water to make a buffer solution with a pH value of 9.0 and an ionic strength of 1.6 mol / L, That is, stripping the aqueous phase solution.

[0051] Add ultrafiltrate, surfactant and THF accounting for 15% v / v of surfactant into a supercritical extractor for supercritical extraction. The surfactant is a mixture of AOT and DTDPA with a molar ratio of 7:3. Surfactants and CO in supercritical extractors 2 Su...

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Abstract

The invention relates to a preparation method of L-tyrosine, belonging to the field of biotechnology. The preparation method of L-tyrosine comprises the following steps: inoculating a culture medium with brevibacterium and performing fermentation culture to obtain fermentation liquid; performing centrifugation and ultrafiltration of the fermentation liquid to obtain ultrafiltration liquid; adjusting the pH value and ion strength of the ultrafiltration liquid; adding a surfactant and the ultrafiltration liquid into a supercritical extractor, wherein the surfactant and supercritical fluid form a supercritical fluid reverse micelles system for supercritical extraction of L-tyrosine; adding the back-extraction water-phase solution and extraction product into the supercritical extractor for supercritical back extraction; and after the back extraction, fetching the water phase and performing nanofiltration, crystallization and freeze drying to obtain purified L-tyrosine. The separation and purification method provided by the invention has high efficiency in extraction separation and extraction, and the obtained L-tyrosine has high activity and purity as well as good water solubility.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a preparation method of L-tyrosine. Background technique [0002] L-tyrosine (L-Tyrosine) is an important biochemical reagent and the main raw material for the synthesis of polypeptide hormones, antibiotics, L-dopa and other drugs, and is widely used in medicine, food additives, biochemical industry and feed, etc. In the field of medicine, it can be used as a treatment for hyperthyroidism. At present, there are mainly three methods for preparing L-tyrosine: hydrolysis, fermentation and synthesis; hydrolysis is the most important production method, but because there are many kinds of amino acids in the hydrolyzate, it is difficult to obtain them by traditional separation and purification methods. Products with higher purity will cause the consumption of raw materials and auxiliary materials and the loss of products, and the product quality is difficult to reach the standa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/22C07C229/36C07C227/40C12R1/13
CPCY02P20/54
Inventor 王裕祥
Owner NINGBO YUANFA BIOENG
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