Mixture, dissolving solution and pharmaceutical agent each comprising thermophilic microorganism

a technology of thermophilic microorganisms and pharmaceutical agents, which is applied in the direction of bacteria based processes, immunological disorders, metabolism disorders, etc., can solve the problems of not being able to achieve the advantageous effect of enhancing muscle-building effects of conventional techniques at the same time, and not being able to disclose direct effects in patent documents

Inactive Publication Date: 2012-11-29
JAPAN ECO SCI +5
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Benefits of technology

[0016]Patent Document 8: JP 2003-219864 A
[0022]Non-Patent Document 6: Journal of Intestinal Microbiology 22: 109-114, 2008
[0023]However, the techniques disclosed in the respective patent and non-patent documents are insufficient in data for interactive verification between the mechanisms of action about influence on animals and the influence to be affected on general health conditions. In particular, the conventional techniques using thermophilic microorganisms do not relate to the results of researches with experimental animals, and are insufficient in findings about fundamental researches or the like that intend to apply the techniques to animals other than farm animals, specifically to humans.
[0024]The present invention has been made in consideration of the above situation, and intends to provide a mixture, a dissolving solution and a pharmaceutical agent using a thermophilic microorganism, which allow regulation of a mucous membrane immune system gene cluster and metabolism-related gene clusters of the intestines and liver based on the data of researches using rats and mice which are experimental animals with accumulated universal data.
[0025]A mi

Problems solved by technology

However, the mechanisms of action of these techniques are not described in detail.
In other words, these patent documents do not disclose direct effects of administration of thermophilic microorganism to an animal on a living body, particularly an influence thereof on the immune or endocrine s

Method used

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  • Mixture, dissolving solution and pharmaceutical agent each comprising thermophilic microorganism
  • Mixture, dissolving solution and pharmaceutical agent each comprising thermophilic microorganism
  • Mixture, dissolving solution and pharmaceutical agent each comprising thermophilic microorganism

Examples

Experimental program
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Effect test

example 1

(1-1) Preparation of Dissolving Solutions 1-A and 1-B

[0058]A dissolving solution 1-A was prepared using a high-temperature fermentation product reported by Niisawa et al. (Niisawa C, Oka S, Kodama H, Hirai M, Kumagai Y, Mori K, Matsumoto J, Miyamoto H, Miyamoto H (2008) Microbial analysis of composted product of marine animal resources and isolation of antagonistic bacteria to plant pathogen from the compost. J Gen Appl Microbiol 54: 149-158) such that the product was diluted 200 times by weight and then subjected to aeration by diffused air at 60 to 70° C. for 6 hours or more. Furthermore, a dissolving solution 1-B was prepared by co-cultivating thermophilic microorganisms included in the dissolving solution 1-A with PTA-1773.

(1-2) Analysis of Microorganisms in Dissolving Solution 1-A

[0059]The dissolving solution 1-A includes various kinds of thermophilic microorganisms, and thermophilic mixed bacteria BP-1051 as the dominant bacterial species. Their base sequences (16SrDNA sequenc...

example 2

(2-1) Preparation of Dissolving Solution 2

[0068]A dissolving solution 2 was prepared by fermenting an organic material containing marine residues with microorganisms included in the dissolving solution 1-B in an air permeable three-staged fermenter installed in Miroku Co., Ltd at 70° C. or more and 90° C. or less, diluting the final fermentation product 100 times with water, and dissolving it therein at 60° C. or less for 10 hours or more under aeration conditions.

(2-2) Analysis of Microorganism Dominant in Cecal Feces of Germ-Free Mouse with Administration of Dissolving Solution 2

[0069]A dissolving solution 2 at a concentration of 0.5% was administered to aseptically breeding Balb / c mice (male, 10 weeks of age) for three weeks, and the base sequences (16SrDNA sequences) of microorganisms isolated from cecal feces of the mice were then analyzed. Here, the above Balb / c mice were bred in isolators (manufactured by ICM Co., Ltd.) in a breeding room controlled at a room temperature of 2...

example 3

(3-1) Verification Experiment [1] for Dissolving Solution 1-A

[0077]An experiment for verifying a regulatory effect of the dissolving solution 1-A on expression of gene cluster by administration of the dissolving solution 1-A to Wistar rats (male, 3 weeks of age) (obtained from Kyudo Co., Ltd.). In this experiment, the following three groups were prepared and compared with one another.

Group (1-A): A normal feeding group (controls)

Group (1-B): A group in which the dissolving solution 1-A was added to drinking water.

Group (1-C): A group in which the dissolving solution 1-A (but sterilized with 0.02 μm) was added to drinking water.

[0078]Here, the Wistar rats used in the experiment were preliminarily bred in groups (1-A) to (1-C) for five days. Furthermore, the Wistar rats were divided into five animals per group and each was bred in its own gauge (manufactured by Natsume Seisakusyo Co., Ltd.). Furthermore, the feed used was one sterilized by radiation (product name MF, manufactured by O...

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Abstract

To provide a mixture, a dissolving solution and a pharmaceutical agent, which contain a thermophilic microorganism to make it possible to regulate a mucous membrane immune system gene cluster and metabolism-related gene clusters of the intestines and the liver. Each of them is prepared by fermentation of an organic material containing a thermophilic microorganism at a temperature of 50° C. or more and 90° C. or less. By being administered to the animal, it regulates at least one of a mucous membrane immune system gene cluster, a metabolism-related gene cluster in the intestines, and a metabolism-related gene cluster in the liver of the animal. The microorganism includes at least one species of the genus Bacillus, Oceanobacillus, Paenibacillus, Anoxybacillus, Lysinibacillus, Methanopyrus, Geogemma, Pyrolobus, Pyrodictium, Hyperthermus, Pyrococcus, Pyrobaculum, Thermococcus, Aeropyrum, Aquifex, Thermotoga, Thermodesulfobacterium, Thermus, Geobacillus, and Thermomyces.

Description

CROSS REFERENCE TO RELATED APPLICATION[0001]This application is the 35 U.S.C. §371 national stage of PCT application PCT / JP2011 / 052,735, filed Feb. 9, 2011, which claims priority to and the benefit of Japanese Patent Application No. 2010-028204, filing date Feb. 10, 2010 and Japanese Patent Application No. 2010-028205, filing date Feb. 10, 2010, both of which are hereby incorporated by reference.TECHNICAL FIELD[0002]The present invention relates to a mixture, a dissolving solution and a pharmaceutical agent each comprising a thermophilic microorganism, which are capable of activating mucous membrane immune systems and regulating metabolisms of animals including humans.BACKGROUND ART[0003]Probiotics using microorganisms have been known to improve the enterobacterial flora of animals, prevent diarrhea, activate immunity, and so on. For example, Patent Document 1 discloses pasteurized ingredients derived from bacterial cells to prevent diarrhea in animals. Also, Patent Document 2 discl...

Claims

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Application Information

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IPC IPC(8): A61K35/74A61P37/04A61P3/00A61K35/741
CPCA61K35/741C12R1/07C12R1/01A61P1/00A61P1/16A61P3/00A61P3/06A61P31/00A61P37/02A61P37/04A61P43/00C12N1/205C12R2001/01C12R2001/07
Inventor MIYAMOTO, HIROKUNIKODAMA, HIROAKINISHIUCHI, TAKUMIMATSUSHITA, TERUOMIYAMOTO, HISASHIHORIUCHI, SANKICHISETA, MANAMIMORI, KENICHIHATTORI, MASAHIRAOGAWA, KAZUO
Owner JAPAN ECO SCI
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