Compound microbial culture for degrading benzene, methylbenzene and xylene waste gas as well as preservation and activation methods of compound microbial culture

A technology of compound microorganisms and strains, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as the degradation of target pollutants, achieve significant environmental and economic benefits, and realize industrial-scale production. , the effect of rapid reproduction

Active Publication Date: 2019-02-15
GUANGDONG ZHONGWEI ENVIRONMENTAL PROTECTION BIOTECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Another object of the present invention is to provide a high-activity preservation and activation method of compound microbial strains that can efficiently degrade "triphenyl" waste gas. By using this method to preserve and activate microbial strains, it can solve the problem of conventional preservation methods. The problem of the degradation of biodegradability can be continuously maintained, and the bioenzyme activity in the cells can still be maintained after activation, which can quickly provide highly active microbial strains for practical engineering applications of bioprocessing

Method used

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  • Compound microbial culture for degrading benzene, methylbenzene and xylene waste gas as well as preservation and activation methods of compound microbial culture
  • Compound microbial culture for degrading benzene, methylbenzene and xylene waste gas as well as preservation and activation methods of compound microbial culture
  • Compound microbial culture for degrading benzene, methylbenzene and xylene waste gas as well as preservation and activation methods of compound microbial culture

Examples

Experimental program
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Effect test

Embodiment 1

[0027] In this embodiment, a compound microbial strain for degrading "triphenyl" waste gas includes the following components in parts by weight: 50 parts of Bacillus, 20 parts of Pseudomonas, 20 parts of Streptococcus, and 20 parts of Rhodococcus 15 and Brevibacterium 18.

[0028] A method for preserving the above-mentioned compound microbial strains for degrading "triphenyl" waste gas, comprising the steps of:

[0029] (A1) The composite microbial strains for degrading "triphenyl" waste gas were inoculated in a sterile enrichment medium with only "triphenyl" as a carbon source at an inoculum size of 10%, and cultured with shaking at 32°C for 24 hours to obtain bacteria liquid;

[0030] (A2) Take the bacterial solution, protective agent and active stabilizer, mix them in a weight ratio of 1:1:1, mix them evenly and store them at -80°C.

[0031] Further, in the step (A1), the sterile enrichment medium includes the following components in mass percent concentration: 0.1% benze...

Embodiment 2

[0051] In this embodiment, a composite microbial strain for degrading "triphenyl" waste gas includes the following components in parts by weight: 40 parts of Bacillus, 13 parts of Pseudomonas, 28 parts of Streptococcus, and 28 parts of Rhodococcus 13 and Brevibacterium 28.

[0052] A method for preserving the above-mentioned compound microbial strains for degrading "triphenyl" waste gas, comprising the steps of:

[0053] (A1) The composite microbial strains for degrading "triphenyl" waste gas were inoculated in a sterile enrichment medium with only "triphenyl" as a carbon source at an inoculum size of 9%, and cultured with shaking at 31°C for 25 hours. The rotating speed is 150rpm, obtains bacterial liquid;

[0054] (A2) Take the bacterial solution, protective agent and active stabilizer, mix them according to the weight ratio of 1:0.8:1.2, mix them evenly and store them at -78°C.

[0055] Further, in the step (A1), the sterile enrichment medium includes the following compon...

Embodiment 3

[0064] In this embodiment, a composite microbial strain for degrading "triphenyl" waste gas includes the following components in parts by weight: 60 parts of Bacillus, 28 parts of Pseudomonas, 13 parts of Streptococcus, Rhodococcus 28 and Brevibacterium 13.

[0065] A method for preserving the above-mentioned compound microbial strains for degrading "triphenyl" waste gas, comprising the steps of:

[0066] (A1) The composite microbial strains for degrading "triphenyl" waste gas were inoculated at an inoculum size of 11% in a sterile enrichment medium with only "triphenyl" as a carbon source, and cultured with shaking at 33°C for 23 hours. The rotating speed is 250rpm, obtains bacterial liquid;

[0067] (A2) Take the bacterial solution, protective agent and active stabilizer, mix them according to the weight ratio of 1:1.2:0.8, mix them evenly and store them at -82°C.

[0068] Further, in the step (A1), the sterile enrichment medium includes the following components in mass pe...

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Abstract

The invention relates to the technical field of sewage treatment, in particular to a compound microbial culture for degrading benzene, methylbenzene and xylene waste gas as well as a preservation method and an activation method of the compound microbial culture. The compound microbial culture comprises a bacillus, a pseudomonas, a streptococcus, a rhodococcus and a brevibacterium. The preservationmethod of the culture comprises the following steps: A1, inoculating 9-11% of inoculum dose of the compound microbial culture for degrading the benzene, methylbenzene and xylene waste gas into an aseptic enrichment medium for shake culturing to form a bacterium solution, and A2, uniformly mixing and storing the bacterium solution, a protective agent and an active stabilizing agent at -78-82 DEG C. The compound microbial culture can efficiently degrade the benzene, methylbenzene and xylene waste gas; the preservation method of the compound microbial culture is simple in technology, easy to control and low in cost; the activated compound microbial culture after preservation can continuously keep good benzene, methylbenzene and xylene degradation capability; the highly degrading benzene series degradation microbial culture is provided for quick practical engineering applications of biotreatment of the benzene series waste gas.

Description

technical field [0001] The invention relates to the technical field of sewage treatment, in particular to a composite microbial strain for degrading "triphenyl" waste gas and a preservation and activation method thereof. Background technique [0002] In the prior art, the microbial strains that degrade "triphenyl" waste gas are not ideal for the degradation of "triphenyl" waste gas, and the preservation process of microbial strains is often accompanied by a decline in production activity, long adaptation time, and slow growth Therefore, the use of routinely preserved microbial strains in the bioremediation process often requires a long-term debugging process, and sometimes even the restoration effect is not good. Therefore, it is an inevitable requirement for bioremediation to obtain high-activity composite microbial strains that degrade "triphenyl" waste gas, and to preserve and activate the high-activity strains. Contents of the invention [0003] In order to overcome t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/04B01D53/72B01D53/84C12R1/07C12R1/38C12R1/46C12R1/13C12R1/01
CPCB01D53/72B01D53/84B01D2257/7027C12N1/04C12N1/20Y02A50/20
Inventor 张保安杨秋婵段磊赖柏民罗仕宽陈浩鹏黄来云
Owner GUANGDONG ZHONGWEI ENVIRONMENTAL PROTECTION BIOTECH CO LTD
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