Composite microbial agent for biological drying of kitchen waste and preparation method thereof

A compound bacterial agent and biological drying technology, applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the impact on the scope and effect of the resource utilization of kitchen waste, affect the efficiency of aerobic composting and the final quality, inability to fully degrade macromolecular organic matter, etc., to achieve the effect of reducing moisture content, shortening time, and improving quality

Pending Publication Date: 2021-04-30
ZHONGNONGXINKE SUZHOU ORGANIC RECYCLING RES INST CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the characteristics of high water content, high salt content, and high oil content of kitchen waste make it necessary to add a large amount of auxiliary materials such as sawdust and straw during the composting process, and it also seriously affects the aerobic composting fermentation process of kitchen waste, which seriously affects the quality of food waste. Oxygen composting efficiency and fina

Method used

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  • Composite microbial agent for biological drying of kitchen waste and preparation method thereof
  • Composite microbial agent for biological drying of kitchen waste and preparation method thereof
  • Composite microbial agent for biological drying of kitchen waste and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] (1) Activation of bacteria

[0032] Bacillus licheniformis (Bacillus licheniformis), Geobacillus thermoleovorans (Geobacillus thermoleovorans), methylotrophic Bacillus (Bacillus methylotrophicus) were inoculated in LB solid medium under sterile conditions, respectively, at 50°C, 70°C, Culture at 60°C for 1 day;

[0033] (2) Preparation of seed bacterial liquid:

[0034] Under sterile conditions, use the inoculation loop to pick 2-3 loops of the activated bacteria in the above step and inoculate them in LB liquid medium, where Bacillus licheniformis is cultured at 50°C and 180 rpm for 2 days , prepared into Bacillus licheniformis seed solution; high-temperature alkane Geobacillus was cultured for 3 days at 70°C and 180 rpm to prepare high-temperature alkane Geobacillus seed solution, and methylotrophic Bacillus was cultured at 60°C and 180 rpm Cultivate for 2 days under the condition of rev / min to prepare methylotrophic bacillus seed liquid;

[0035] (3) Liquid fermen...

Embodiment 2

[0041] (1) Activation of bacteria

[0042]Bacillus licheniformis (Bacillus licheniformis), Geobacillus thermoleovorans (Geobacillus thermoleovorans), methylotrophic Bacillus (Bacillus methylotrophicus) were inoculated in LB solid medium under sterile conditions, respectively, at 40°C, 70°C, Culture at 50°C for 1 day;

[0043] (2) Preparation of seed bacterial liquid:

[0044] Under sterile conditions, use the inoculation loop to pick 2-3 loops of the activated bacteria in the above step and inoculate them in LB liquid medium, where Bacillus licheniformis is cultured at 40°C and 180 rpm for 2 days , prepared into Bacillus licheniformis seed solution; high-temperature alkane Geobacillus was cultivated for 3 days at 70°C and 180 rpm to prepare high-temperature alkane Geobacillus seed solution, and methylotrophic Bacillus was cultured at 50°C and 180 rpm Cultivate for 2 days under the condition of rev / min to prepare methylotrophic bacillus seed liquid;

[0045] (3) Liquid ferme...

Embodiment 3

[0051] (1) Activation of bacteria

[0052] Bacillus licheniformis (Bacillus licheniformis), Geobacillus thermoleovorans (Geobacillus thermoleovorans), methylotrophic Bacillus (Bacillus methylotrophicus) were inoculated in LB solid medium under sterile conditions, respectively, at 40°C, 60°C, Culture at 50°C for 1 day;

[0053] (2) Preparation of seed bacterial liquid:

[0054] Under sterile conditions, use the inoculation loop to pick 2-3 loops of the activated bacteria in the above step and inoculate them in LB liquid medium, where Bacillus licheniformis is cultured at 40°C and 180 rpm for 2 days , prepared into Bacillus licheniformis seed solution; high-temperature alkane Geobacillus was cultured for 3 days at 60°C and 180 rpm to prepare high-temperature alkane Geobacillus seed solution, and methylotrophic Bacillus was cultured at 50°C and 180 rpm Cultivate for 2 days under the condition of rev / min to prepare methylotrophic bacillus seed liquid;

[0055] (3) Liquid fermen...

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Abstract

The invention discloses a composite microbial agent for biological drying of kitchen waste, which comprises bacillus licheniformis, high-temperature alkane geobacillus and bacillus methylotrophicus, the viable count of the bacillus licheniformis is 30-40%, the viable count of the high-temperature alkane bacillus is 30-50%, and the viable count of the bacillus methylotrophicus is 20-40%; the bacillus licheniformis VH11 is preserved in the China General Microbiological Culture Collection Center on November 26, 2020, and the preservation number of the bacillus licheniformis VH11 is CGMCC (China General Microbiological Culture Collection Center) No. 21231. The microbial composite inoculant can effectively solve various problems in the reduction, stabilization, harmlessness and resource utilization process of the kitchen waste.

Description

technical field [0001] The invention belongs to the field of microbial bacterial agents, and in particular relates to a microbial composite bacterial agent used for biological drying of kitchen waste. Background technique [0002] With the rapid growth of my country's economy, the acceleration of urbanization and the general improvement of people's living standards, the generation of urban domestic waste has also increased, of which the growth of kitchen waste is faster; in megacities such as Beijing, food waste disposal The proportion is as high as 60%, and the highest can reach more than 80%. The traditional treatment methods of food waste are mostly incineration or landfill, but due to its high water content, high oil and salt content, and perishability, it can only be incinerated with other waste such as domestic waste, which greatly reduces the heat energy of incineration. At the same time, in the incineration process, the lipids contained in it will produce toxic and h...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05F15/00C05F17/20B09B3/00C12R1/10C12R1/07
CPCC12N1/20C05F9/00C05F17/20B09B3/00C05F11/00C05F11/08Y02W30/40
Inventor 李季丁国春丁晓艳孟雅
Owner ZHONGNONGXINKE SUZHOU ORGANIC RECYCLING RES INST CO LTD
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