High-temperature degrading bacteria and application thereof

A high-temperature degradation and bacterial strain technology, applied in the field of biodegradation and microorganisms, can solve the problems of difficult degradation of organic macromolecules, small number of microorganisms, etc.

Active Publication Date: 2013-10-23
武汉沃土恒通环境工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a strain that can grow at high temperature and effectively degrade organic macromolecules for the problem that the types and quantities of microorganisms in the high temperature period of composting are difficult to degrade organic macromolecules

Method used

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  • High-temperature degrading bacteria and application thereof
  • High-temperature degrading bacteria and application thereof
  • High-temperature degrading bacteria and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1 Isolation of Geobacillus HN-5

[0017] Collect the filter mud compost samples from Hainan Yuwo Biotechnology Company in Chengmai County, Hainan Province and stack them for 10 days. After several generations of high-temperature acclimation, the bacterial solution was applied on the beef extract peptone (NR) plate by gradient dilution method and cultured at 70°C. , isolate high-temperature-resistant strains, and obtain pure bacteria after several generations of streaking and purification. NR formula: beef extract 5g, peptone 10g, NaCl 5g, agar 15g-20g, water 1000mL, pH 7.4-7.6.

[0018] Inoculate multiple strains of isolated pure bacteria into solid selective medium to identify their degradation ability, and observe whether there is a hydrolysis circle around the colony. If there is a hydrolysis circle, it means that the strain has the ability to degrade the substrate, otherwise, it does not have the degradation ability. ability, and finally obtained a Geobacil...

Embodiment 2

[0024] Example 2 Research on Enzyme Production Ability of Geobacillus HN-5

[0025] Prepare HN-5 seed solution (70°C, cultured in NR liquid medium until the OD value is about 0.4), take 1ml of the seed solution and inoculate it into cellulose, protein, starch fermentation medium (recipe below), and culture at 70°C, After 24 hours, the cellulase activity, amylase activity and protease activity of the strains were measured.

[0026] Cellulose fermentation medium: CMC-Na 5g, NaNO 3 2.5g, KH 2 PO 4 1g, MgSO 4 0.6g, NaCl 0.1g, CaCl 2 0.1g, FeCl 3 0.01g, gelatin 2g, yeast powder 0.1g, H 2 O1000mL, pH 6.8-7.2.

[0027] Starch fermentation medium: 10g soluble starch, 10g peptone, 5g yeast powder, 10g NaCl, pH 7.0, 15g-20g plant gel, 0.05-0.1g Triben blue, H 2 O 1000mL, sterilized at 121°C for 30min.

[0028] Protein fermentation medium: casein 5%, Mandel A salt 10%, Mandel B salt 1%, pH6.0; Mandel A salt: KH 2 PO 4 2g / L, (NH 4 ) 2 SO 4 1.4g / L, MgSO 4 0.3g / L, CaCl...

Embodiment 3

[0033] Example 3 Growth Curve of Geobacillus HN-5

[0034] Take a fresh bacterial culture plate of the bacteria to be tested, pick 1 ring of bacterial lawn by aseptic operation, insert it into a 250ml Erlenmeyer flask containing 100ml of culture solution, and place it at 70°C for static culture for 24h as the seed culture solution. Use a sterile pipette to draw 1ml of seed culture solution into a 250ml Erlenmeyer flask filled with 100ml of sterilized culture solution, and culture at 70°C. Take the culture solution every 4 hours and measure the absorbance value under the 660nm wavelength, take the time as the abscissa, and the OD value as the ordinate to draw the growth curve of the bacterial strain ( image 3 ).

[0035] Experimental results such as image 3 As shown, HN-5 has a short incubation period, enters the logarithmic growth phase soon, and the logarithmic phase lasts longer (≥24h), and the strain stops growing after 36h.

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Abstract

The invention provides a high-temperature degrading bacteria which is a geobacillus (Geobacillus) strain HN-5 and has a collection number of CGMCC (China General Microbiological Culture Collection Center) NO.5852. The bacteria can simultaneously degrade three high polymer organic matters such as cellulose, protein and starch under the aerobic conditions of about 70 DEG C, and microbial resources and technical support are provided for high-temperature aerobic composting and rapid rot.

Description

technical field [0001] The invention relates to the field of microorganisms and biodegradation, in particular to a high-temperature degradation bacterium and its application. Background technique [0002] Thermobacteria are a class of microorganisms that live in high-temperature environments. In nature, high-temperature bacteria are usually distributed in geothermal areas and artificial high-temperature environments. There are three types of geothermal environments where high-temperature bacteria live: (1) acidic sulfur areas. It is rich in sulfur, acidic soil, acidic hot springs and boiling mud ponds. (2) Freshwater hot springs and neutral and alkaline geysers. (3) Deep sea craters. The pH is neutral, and the temperature is between 20°C and 350°C. In addition, the organisms in various ponds, soil, haystacks, and manure heated by solar energy can also reach a relatively high temperature, but the maintenance time is short, and it is only suitable for fast-growing spores....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N9/42C12N9/26C12N9/50C05F11/08C05F17/00C12R1/01
CPCY02W30/40
Inventor 李季程旭艳任莉王定美
Owner 武汉沃土恒通环境工程有限公司
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