Perishable organic solid waste biological drying strain and application thereof

A technology of organic solid waste and solid microbial agent, applied in the field of environmental microorganisms, can solve the problems of short high temperature duration, poor drying effect, and few types of high-efficiency functional microorganisms, and achieve the effect of strong self-heating ability

Active Publication Date: 2021-04-06
SHANGHAI ADVANCED RES INST CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Each method has its own advantages and disadvantages: (1) Dry incineration: It can completely oxidize and decompose the organic components in organic solid waste, and the reduction rate is as high as 50%-80%
However, the biological drying of perishable organic solid waste still has technical problems such as slow start, short duration of high temperature, and poor drying effect.
The main reason for this problem is due to the high temperature in the indigenous microorganisms of the raw materials, the few types of highly efficient functional microorganisms, the low concentration, and the poor activity

Method used

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  • Perishable organic solid waste biological drying strain and application thereof
  • Perishable organic solid waste biological drying strain and application thereof
  • Perishable organic solid waste biological drying strain and application thereof

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preparation example Construction

[0044] The third aspect of the present invention provides the preparation method of the liquid microbial agent, at least including the following steps: making Geobacillus thermophilic Geobacillus kaustophilus The pure strain of FWGK-JYJ1 is inoculated in the liquid medium for cultivation, and the liquid bacterial agent is obtained after the cultivation is completed.

[0045] In one embodiment, the Geobacillus thermophilus Geobacillus kaustophilus After the pure strain of FWGK-JYJ1 is inoculated into a liquid medium for cultivation, the obtained culture solution is then inoculated into another liquid medium for expanded cultivation, and a liquid bacterial agent is obtained after the cultivation is completed.

[0046] In one embodiment, the temperature of cultivation is 40-60°C.

[0047] In one embodiment, the stirring speed of the cultivation is 150-230 r / min.

[0048] In one embodiment, the Geobacillus thermophilus Geobacillus kaustophilus The pure strain of FWGK-JYJ1 was i...

Embodiment 1

[0069] Example 1: Isolation, screening and performance determination of bacterial strain FWGK-JYJ1

[0070] The separation and purification process of the bacterial strain FWGK-JYJ1 of the present invention: the test sample was collected from a high-temperature aerobic fermentation tank of a catering waste treatment plant, mixed with sterile water, and then diluted in a gradient, and 200 μL of dilutions of different gradients were applied to the After culturing in a constant temperature incubator at 50 °C for 24-48 hours on the separation and purification medium plate, single colonies with different shapes and sizes were picked, streaked and purified, and numbered and preserved. A total of 41 strains were obtained through preliminary screening.

[0071] The strains obtained from the primary screening were re-screened at the highest growth temperature, and the strains were streaked on the solid medium and placed in an incubator with a temperature gradient of 50, 55, 60, 65, 70, ...

Embodiment 2

[0074] Embodiment 2: the bacterial agent preparation of bacterial strain FWGK-JYJ1

[0075] Preparation of liquid bacterial agent: Inoculate the pure strain of FWGK-JYJ1 into five 10 mL seed culture medium, culture in a constant temperature shaker at 50 °C and 180 r / min for 12 h, and then add 1 volume of the liquid culture medium % of the inoculum was inserted into 5 L liquid medium for expansion and cultured at 50 °C and 180 r / min for 18 h to obtain the liquid inoculum of the strain.

[0076] Preparation of solid bacterial agent: it is prepared by mixing starch and liquid bacterial agent of the strain according to the mass ratio of 1:5, and spray drying.

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Abstract

The invention relates to the technical field of environmental microorganisms, in particular to a perishable organic solid waste biological drying treatment strain and application thereof. The perishable organic solid waste biological drying treatment strain is identified as geobacillus thermophilus, named as geobacillus thermophilus FWG-JYJ1, and has an accession number is CCTCC NO: M 2020974. The geobacillus thermophilus FWGK-JYJ1 is resistant to high temperature and high in self-heat-production capacity, has the capacity of rapidly and efficiently releasing moisture in perishable organic solid waste, can be applied to the biological drying treatment process of perishable organic solid waste such as food waste, kitchen waste, fruit and vegetable waste, vegetable residues, livestock and poultry breeding excrement and municipal sludge, and achieves the effects of rapid drying and stabilization of the perishable organic solid waste.

Description

technical field [0001] The invention relates to the technical field of environmental microorganisms, in particular to a perishable organic solid waste biological drying strain and application thereof. Background technique [0002] With the improvement of people's living standards and the continuous advancement of urbanization in our country, the amount of perishable organic solid waste is increasing year by year. Perishable organic solid waste mainly comes from agricultural waste, industrial waste and domestic waste. Especially since 46 cities in my country started household waste classification, the amount of perishable organic solid waste from domestic waste has risen sharply. Perishable organic solid waste has high moisture content, low calorific value, high organic matter content, and perishable odor, which brings certain difficulties to collection, transportation and terminal treatment and disposal. If it is not treated effectively, it will easily cause the reproducti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09B3/00C02F11/02C02F11/12C12R1/01
CPCB09B3/00C02F11/02C02F11/12C12N1/20
Inventor 刘莉吉雨霁史吉平王娜张斯颖陈晓淼刘乾静
Owner SHANGHAI ADVANCED RES INST CHINESE ACADEMY OF SCI
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