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Geobacillus thermoleovorans, bacterial agents thereof and application in kitchen waste treatment

A technology of Geobacillus and kitchen waste, applied in the field of environmental microorganisms, can solve problems such as safety, impact on residents' living environment and health, environmental pollution, etc., and achieve efficient degradation effect

Active Publication Date: 2020-05-15
上海创净生物环保科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, food waste is mainly used to raise livestock, and the rest is mostly mixed with urban waste for landfill, incineration or composting, which is likely to cause serious environmental pollution and safety problems, and greatly affects the living environment and health of residents.

Method used

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  • Geobacillus thermoleovorans, bacterial agents thereof and application in kitchen waste treatment
  • Geobacillus thermoleovorans, bacterial agents thereof and application in kitchen waste treatment
  • Geobacillus thermoleovorans, bacterial agents thereof and application in kitchen waste treatment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Isolation, screening and performance determination of strain CJ-8

[0039] Separation and purification: The test samples were collected from an aerobic fermentation tank of a food waste treatment plant. After taking an appropriate amount of samples and mixing them with sterile water, they were diluted in a gradient. Take 200 μL of the dilutions of different gradients and spread them on the separation and purification medium plate Above, after culturing in a constant temperature incubator at 55°C for 24 hours, single colonies with different shapes and sizes were picked, streaked and purified, and then numbered and stored. A total of 14 strains were obtained through preliminary screening.

[0040]Re-screening: Inoculate the pure strains obtained from the primary screening onto the protease screening medium and lipase screening medium by spot-patch method, and measure the enzyme-producing ability of the strains, so as to carry out re-screening. Incubate the spot-connected ...

Embodiment 2

[0044] Preparation of liquid inoculum

[0045] Inoculate CJ-8 pure bacteria into 50 mL of liquid medium, culture it in a constant temperature shaker at 37 °C and 180 r / min for 16-24 h, and then inoculate it into the same In the liquid culture medium, the liquid microbial agent of the strain can be obtained by expanding the culture under the same culture conditions.

Embodiment 3

[0047] Preparation of solid bacterial agent

[0048] The above-mentioned liquid microbial agent of the strain is prepared by mixing and drying low-value biomass carriers such as wood chips, rice husks, and wheat bran, wherein the mass ratio of the carrier to the liquid microbial agent is 1: (1~5).

[0049] In the present invention, other suitable carriers can also be added to prepare other types of bacterial agents.

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PUM

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Abstract

The invention belongs to the technical field of environmental microbiology and provides a novel strain with the Latin name of Geobacillus thermoleovorans collected in China Center for Type Culture Collection (CCTCC) with a collection number of CCTCC M 20191066. Meanwhile, the invention also provides a liquid bacterial agent and a solid bacterial agent prepared from the strain. The invention further provides application of the strain in kitchen waste treatment. The strain can efficiently and rapidly degrade various organic matters in the kitchen waste under a high temperature condition and canbe used for rapid reduction treatment of the kitchen waste to realize rapid biological reduction and stabilization of the kitchen waste. The strain has remarkable aerobic biological treatment effectson the kitchen waste, can significantly reduce the treatment cost and has higher application value in the kitchen waste treatment field.

Description

technical field [0001] The invention belongs to the technical field of environmental microorganisms, and in particular relates to Geobacillus thermooleophilus and its bacterial agent and its application in the treatment of kitchen waste. Background technique [0002] In recent years, with the continuous advancement of my country's urbanization process and the improvement of people's living standards, my country's catering industry has also continued to develop, and the amount of food waste has also shown a trend of increasing year by year, and has become the main component of urban domestic waste. Food waste has the characteristics of high moisture content (up to 60%~80%), high proportion of organic matter (accounting for 95%~98% of dry weight), and high salt content. The environmental pollution caused by food waste is also expanding. For example, it is very perishable during storage and transportation, causing sour odor. At the same time, it may produce a large amount of to...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N11/02B09B3/00C12R1/01
CPCB09B3/00C12N1/20C12N11/02C12N1/205C12R2001/01Y02W30/78
Inventor 郑会一
Owner 上海创净生物环保科技有限公司
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