In-situ microbial oxygen generation and hydrocarbon conversion in a hydrocarbon containing formation
a hydrocarbon containing, in-situ oxygen technology, applied in the direction of fermentation, fluid removal, borehole/well accessories, etc., can solve the problems of hampered utilization of microorganisms to enhance hydrocarbon recovery, and achieve enhanced hydrocarbon recovery, improved quality, and enhanced bioavailability and biodegradability
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[0050]A suitable embodiment of the method according to the invention, comprises the following steps:
a) Screening whether a target underground crude oil and / or natural gas containing reservoir formation has features, such as temperature, salinity, heterogeneity, oil characteristics, micro-organisms, volatile fatty acids, hydrogen ions, acetate, propionate or butyrate and / or other potential electron donors, etc., which allow use of the method according to the invention;
b) Analyzing the composition of the water, oil and / or natural gas in the formation, for example by screening a sample taken from the formation;
c) Identification of potentially interesting micro-organisms with molecular DNA technologies using either general (16S rRNA-related) primer sets or enzyme / functional group specific primer sets (nitrate / nitrite-reductase, (per)chlorate reductases, chlorite dismutase, or hydrocarbon (alkane) degrading enzymes or using metagenomics;
d) Isolation of potentially interesting indigenous ...
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Description
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Application Information
- IPC
- E21B43/22
- CPC
- C09K8/582; C12P3/00; C12P1/04; C09K8/592; C09K8/60
- Inventors
- LOMANS, BARTHOLOMEUS PETRUS

