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A Strain of Geobacillus oleophilus g1201 and Its Application

A geobacillus and spore technology, applied to a strain of geobacillus oleophilus G1201 and its application field, can solve the problems of long period of natural fermentation, lack of decomposed strains, odor, etc., to improve germination rate, reduce Odor, the effect of broad application prospects

Active Publication Date: 2020-11-20
QINGDAO MARINE BIOPHARMACEUTICAL RES INST +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, in the process of fermenting and producing organic fertilizer, the natural fermentation method has problems such as too long cycle, serious odor and lack of special decomposing strains.

Method used

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  • A Strain of Geobacillus oleophilus g1201 and Its Application
  • A Strain of Geobacillus oleophilus g1201 and Its Application
  • A Strain of Geobacillus oleophilus g1201 and Its Application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1, Identification of Geobacillus oleophilus G1201

[0026] 1. Morphological identification

[0027] Refer to "Bergey's OF Systematic Bacteriology Second Edition "VolumeThree" carried out morphological identification on G1201, and observed its morphology with transmission electron microscope. The strain was yellowish on LB solid medium, translucent, about 3-4 mm in diameter, irregular, with nicks on the edges ( figure 1 ). Under the transmission electron microscope, the cells are rod-shaped, 0.8-1.2 μm wide, 3.0-3.5 μm long, terminal spores, with flagella ( figure 2 ).

[0028] 2. Amplification and sequence analysis of 16S rRNA gene

[0029] 1) The bacterial genome was extracted referring to the operating instructions of the kit; 2) The genome extracted in the above step was used as a template, and the bacterial universal primer 27F (5'-AGAGTTTGATCCTGGCTCAG-3', SEQ ID No: 2) and 1492R (5'-GGTTACCTTGTTACGACTT- 3', SEQ ID No: 3) for PCR amplification. The ...

Embodiment 2

[0030] Embodiment 2, protease characteristics of Geobacillus oleophilus G1201

[0031] Geobacillus oleophilus G1201 was inoculated on a solid medium plate of skim milk powder, cultured at 65°C for 48 hours, and its protease activity was observed by the size of the transparent circle ( image 3 ). Protease activity was measured by the GB / T23527-2009 Folin phenol method.

[0032] Table 1 Results of enzyme production on bacterial strain G1201 plate

[0033]

Embodiment 3

[0034] Example 3, Preparation of Geobacillus oleophilus G1201 liquid fermentation agent

[0035] Take the preserved Geobacillus oleophilus G1201 and activate it in LB liquid medium, culture it on a shaker at 50-70°C and 180-200rpm for 12-18 hours, and inoculate 30L Fermentation tank, cultivated under aeration conditions for 18 hours, used as seed liquid, and then inoculated in a 1000L fermentation tank with an inoculum amount accounting for 1% of the total mass of the medium, cultured for more than 6 hours, and the cell density was greater than or equal to 10 6 CFU / mL.

[0036] Table 2 Bacteria density under different culture conditions

[0037]

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Abstract

The invention discloses a strain of Geobacillus oleophilus ( Geobacillus thermoleovorans ) G1201 and its application, involving the field of microorganisms, the strain preservation number is CGMCC No.17647. The thermophilic Geobacillus oleophilus G1201 can be applied to the degradation of feathers and feather meal, the treatment of livestock and poultry manure, and the production of organic fertilizers. The leavening agent described in the invention can degrade feathers or feather meal, and the mercapto group content after fermentation is 2 to 4 times that before fermentation, and its degradation products can be used as good feed protein raw materials. The starter is applied to ferment chicken manure, which can obviously remove its odor. The extract of fermented chicken manure is used for seed germination test, and its germination rate is twice that of the control group, which can effectively improve the maturity of organic fertilizer.

Description

technical field [0001] The invention relates to a strain of Geobacillus oleophilus G1201 and its application, in particular to the application of the strain and starter in the fields of degradation of feathers and / or feather meal, preparation of feed protein raw materials, treatment of livestock and poultry manure, and the like. Background technique [0002] Proteases account for about 75% of the types of industrial enzyme preparations, and in recent years sales have accounted for more than 65% of the entire enzyme preparation market. In addition, protease has a wide range of applications and plays an important role in many fields such as food, tanning, feed, washing, textile and medicine. Proteases can be divided into low-temperature proteases, medium-temperature proteases and high-temperature proteases according to their optimum action temperature. The optimum action temperature of high-temperature proteases is 60-80°C or even higher. Since many production processes such ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23K10/12A23K10/26A23K10/18C05F15/00C05F11/08C12R1/01
CPCA23K10/12A23K10/18A23K10/26C05F3/00C05F11/08C12N1/205C12R2001/01C05F17/00
Inventor 何增国刘国瑞戴宝汤伟卢德鹏唐涛孙晓雯张军
Owner QINGDAO MARINE BIOPHARMACEUTICAL RES INST
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