Microbial compound inoculant for improving yield and quality of codonopsis pilosula and preparation method and application thereof

A technology of compound microbial bacterial agent and compound bacterial agent, which is applied in the field of microbial compound bacterial agent and microbial agricultural application, can solve problems such as inability to grow Codonopsis pilosula and enhance stress resistance, and achieve increased germination rate, improved growth status, increased fresh weight and The effect of seedling height

Pending Publication Date: 2021-04-27
LANZHOU UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to provide a microbial compound agent for improving the yield and quality of Codonopsis pilosula and its preparation method and applicatio

Method used

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  • Microbial compound inoculant for improving yield and quality of codonopsis pilosula and preparation method and application thereof
  • Microbial compound inoculant for improving yield and quality of codonopsis pilosula and preparation method and application thereof
  • Microbial compound inoculant for improving yield and quality of codonopsis pilosula and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] A microbial compound bacterial agent that improves the yield and quality of Codonopsis pilosula, said compound microbial bacterial agent includes Pseudomonas fluorescens (Pseudomonas fluorescens), strain preservation number is CGMCC NO: 20094, Geobacillus sp., bacterium The preservation number of the species is CGMCC NO:20095 and the cell recovery promoting factor Rpf protein; the live bacteria ratio of Pseudomonas fluorescens and Geobacillus sp. is 2:3; the cell recovery promoting factor Rpf protein The amount is 0.03 mg / mL.

[0051] In the technical scheme of the present application, the composite microbial agent enhances the viability of Pseudomonas fluorescens and Geobacillus sp. by adding the cell recovery-promoting factor Rpf protein, a highly conserved 70 The structural domain composed of amino acid residues can promote the proliferation of Pseudomonas fluorescens and Geobacillus sp. cells, and is also related to the target antigen recognized by the host immune s...

Embodiment 2

[0059] On the basis of Example 1, the live bacteria ratio of Pseudomonas fluorescens (Pseudomonas fluorescens) and Geobacillus sp. (Geobacillus sp.) is 1:1; All with embodiment 1.

Embodiment 3

[0061] On the basis of Example 1, the live bacteria ratio of Pseudomonas fluorescens (Pseudomonas fluorescens) and Geobacillus sp. (Geobacillus sp.) is 4:1; All with embodiment 1.

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Abstract

The invention discloses a microbial compound inoculant for improving the yield and quality of codonopsis pilosula and a preparation method and application thereof, belongs to the field of microbial agricultural application, and particularly relates to the field of microbial compound inoculants. The defects that an existing microbial inoculant cannot well promote the growth of codonopsis pilosula and enhance stress resistance under normal growth conditions are overcome. The microbial compound agent is prepared from pseudomonas fluorescens with the strain preservation number of CGMCC NO: 20094, geobacillus gemini with the strain preservation number of CGMCC NO: 20095 and a cell resuscitation promoting factor Rpf protein. The microbial compound agent can remarkably promote the growth and metabolism capacity of codonopsis pilosula, the content of soluble sugar and chlorophyll in codonopsis pilosula is remarkably increased under the synergistic effect, the content of the soluble sugar and chlorophyll in codonopsis pilosula is remarkably increased, the fresh weight and the seedling height of the codonopsis pilosula seedlings are increased, particularly the root activity of the codonopsis pilosula and the ability of synthesizing SOD and PPO are improved, the growth condition of the codonopsis pilosula is obviously improved, and the yield and the quality of the codonopsis pilosula are improved.

Description

technical field [0001] A microbial compound bacterial agent for improving the output and quality of Codonopsis pilosula, its preparation method and application, the invention belongs to the technical field of microbial agricultural application, and specifically relates to the technical field of microbial compound bacterial agent. Background technique [0002] With the development of social economy and the improvement of people's living standards, health care has become an important content for people to improve their happiness index. Codonopsis Codonopsis is a perennial herb belonging to the Codonopsis genus of Campanulaceae. It is rich in acetylene glycosides, polysaccharides, proteins, essential amino acids and minerals. The Health Commission included it in the list of homology of medicine and food. In recent years, with the increasing demand for Codonopsis pilosula, wild Codonopsis pilosula can no longer meet the market demand. Due to the influence of geographical enviro...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/27A01N63/22A01N37/46A01P21/00C12R1/39C12R1/01
CPCC12N1/20A01N63/27A01N63/22A01N37/46Y02A40/10
Inventor 王永刚王江勤晏祖花徐也朱宁陈凯冷非凡马建忠陈吉祥
Owner LANZHOU UNIVERSITY OF TECHNOLOGY
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