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Composite bacteria for removing oil from kitchen waste and preparation method for composite bacteria

A technology of food waste and composite bacteria, which is applied in the direction of biochemical equipment and methods, methods based on microorganisms, bacteria, etc., can solve problems such as air pollution, odor generation, and increase the burden on waste disposal sites, achieving small production investment, Effect of reducing odor and low production cost

Active Publication Date: 2012-05-02
SHANDONG SUKAHAN BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, this status quo not only aggravates the burden on the garbage disposal site, but also produces odor due to the microbial decomposition of organic components under anaerobic conditions, resulting in air pollution.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] High-density culture of Trichoderma viride, Bacillus subtilis, Rhizopus niger, photosynthetic bacteria, Candida lipolytica, Bacillus licheniformis, Trichobacterium nitrosos and Nitrobacter vickers:

[0050] (1) Trichoderma viride: Inoculate the Trichoderma viride strain on the purified slant medium into a shake flask filled with an activated medium with a volume ratio of 15%, and the activated medium is wort potato sucrose medium , at a culture temperature of 30°C and a shaker flask rotation speed of 180rpm, activate and cultivate for 32 hours; inoculate the Trichoderma viride strains that have been activated and cultured into a fermenter with a volume ratio of 50% fermentation medium, and the inoculum volume is 1% by volume , the formula of the fermentation medium is glucose 8g / l, corncob 5g / l, calcium chloride 0.2g / l, magnesium sulfate 0.1g / l, potassium dihydrogen phosphate 18g / l, yeast extract 8g / l, sulfuric acid Ferrous 3mg / l, zinc sulfate 1.0mg / l, cobalt chloride 3...

Embodiment 2

[0060] High-density culture of Trichoderma viride, Bacillus subtilis, Rhizopus niger, photosynthetic bacteria, Candida lipolytica, Bacillus licheniformis, Trichobacterium nitrosos and Nitrobacter vickers:

[0061] (1) Trichoderma viride: Inoculate the Trichoderma viride strain on the purified slant culture medium into a shaker flask filled with a 30% volume ratio of activated medium, which is wort potato sucrose medium , at a culture temperature of 28°C and a shaker flask rotation speed of 140rpm, activate and cultivate for 48 hours; inoculate the Trichoderma viride strains that have been activated and cultured into a fermenter with a volume ratio of 70% fermentation medium, and the inoculum volume is 3% by volume , the formula of the fermentation medium is glucose 12g / l, corn cob 6g / l, calcium chloride 0.6g / l, magnesium sulfate 0.5g / l, potassium dihydrogen phosphate 22g / l, yeast extract 12g / l, sulfuric acid Ferrous 7mg / l, zinc sulfate 1.8mg / l, cobalt chloride 4.0mg / l and mang...

Embodiment 3

[0071] High-density culture of Trichoderma viride, Bacillus subtilis, Rhizopus niger, photosynthetic bacteria, Candida lipolytica, Bacillus licheniformis, Trichobacterium nitrosos and Nitrobacter vickers:

[0072] (1) Trichoderma viride: inoculate the Trichoderma viride strain on the purified slant medium into a shaker flask filled with an activated medium with a volume ratio of 20%, and the activated medium is wort potato sucrose medium , at a culture temperature of 30°C and a shaker flask rotation speed of 160rpm, activate and cultivate for 36 hours; inoculate the Trichoderma viride strains that have been activated and cultured into a fermenter with a volume ratio of 60% fermentation medium, and the inoculum volume is 2% by volume , the formula of the fermentation medium is glucose 10g / l, corn cob 5.5g / l, calcium chloride 0.4g / l, magnesium sulfate 0.3g / l, potassium dihydrogen phosphate 20g / l, yeast extract 10g / l, Ferrous sulfate 5mg / l, zinc sulfate 1.5mg / l, cobalt chloride 3...

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Abstract

The invention discloses composite bacteria for removing oil from kitchen waste and a preparation method for the composite bacteria. The preparation method comprises the following steps of: performing high-density culture on strains of trichoderma viride, bacillus subtilis, black rhizopus, photosynthetic bacteria, candida lipolytica, bacillus licheniformis, nitrosomonas europaea and nitrobacter winogradskyi respectively to obtain microbial dry hypopus powder; and mixing 15 to 20 weight parts of trichoderma viride, 10 to 15 weight parts of bacillus subtilis, 25 to 40 weight parts of black rhizopus, 5 to 10 weight parts of photosynthetic bacteria, 5 to 10 weight parts of candida lipolytica, 10 to 15 weight parts of bacillus licheniformis, 5 to 15 weight parts of nitrosomonas europaea and 5 to 15 weight parts of nitrobacter winogradskyi to obtain the composite bacteria for removing the oil from the kitchen waste. The composite bacteria can quickly decompose lipid ingredients in the kitchen waste when used for treating the kitchen waste; and compared with the prior art, the method has the advantages that: the production investment and the production cost are low, and the method is convenient to use.

Description

technical field [0001] The invention relates to the technical field of bioengineering microbial fermentation, in particular to a composite bacterium for removing oil from food waste and a preparation method thereof. Background technique [0002] The organic content of food waste can reach more than 90%, and the content of crude fat is about 25%. Due to the existence of crude fat, the viscosity of food waste is relatively high, which affects the ventilation. Conducive to the fermentation and decomposition of organic components. Conventional kitchen waste lipids are treated by physical and chemical methods, and industrial lipids are extracted from garbage again. This treatment method requires a large investment and high processing costs, and it is impossible to use physical and chemical methods for residential waste mixed with ordinary waste. separated by chemical methods. At present, due to people's awareness of environmental protection, most of the kitchen waste in the cit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00C12N1/14C12N1/16C12N1/20C12R1/01C12R1/125C12R1/885C12R1/845C12R1/73C12R1/10
Inventor 韩威华于伟陈永科
Owner SHANDONG SUKAHAN BIO TECH
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