Harmless oil mud treatment process
A treatment process and technology for sludge, applied in sludge treatment, sludge treatment, biological sludge treatment, etc., can solve the problems of sludge pollution, high operating costs, troublesome operation and management, and achieve low cost and low power consumption. Effect
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Embodiment 1
[0014] Select the gutter sludge near the hotel to screen through the following methods:
[0015] 1.1 Strain enrichment
[0016] Add a certain amount of sludge (3%) to the glucose medium in the Erlenmeyer flask, put it into a shaker, and cultivate at 30°C for 3 days to obtain a bacterial enriched liquid. The glucose medium formula is: sodium chloride 1.2g, calcium chloride 0.7g, dipotassium hydrogen phosphate 0.3g, potassium dihydrogen phosphate 0.3g, ferric chloride 0.05g, ammonium nitrate 1.2g, magnesium sulfate heptahydrate, 1.1g Yeast extract, 1.8g glucose, 1.2L water, PH7.0-7.5; sterilize at 110°C for 20min.
[0017] 1.2 Strain screening
[0018] Dilute the bacterial enrichment solution and then streak it on a glucose solid medium plate (add 2% agar to the glucose medium), culture at 25°C for 24 hours, pick a single colony that grows well on the plate and streak it for inoculation Incubate on an alkane plate at 25°C for 36 hours, pick the two largest single colonies and name the...
Embodiment 2
[0020] Biological and biochemical tests were performed on the Y strain. The yeast YPD agar was cultured on a plate (25°C) for 3 days. The colonies could grow to 1-2 mm, white to cream. The initial colony was smooth, and the colony appeared wrinkled after prolonged cultivation. When grown on a solid or liquid medium, the cell shape is round or oval, and sometimes the cells are elongated and grow like pseudofilaments, and the cell size is about 4-6×6-8 microns.
[0021] Biological and biochemical tests were conducted on the P strain. The bacteria were cultured on a nutrient agar plate for 24 hours. The bacteria grew vigorously, the colonies were smooth, moist, translucent and light yellow colonies, which produced strong odors, and the Gram stain was negative. It has multiple flagella, and most strains have more than three flagella. The bacterium is obligate to aerobic, metabolizes in respiratory type, decomposes glucose and xylose, uses citrate, and phenylalanine dehydrogenase rea...
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