Method for producing liquid organic bacterial manure by using kitchen waste liquid

A technology of organic bacteria and waste liquid, which is applied in the preparation of organic fertilizers, methods based on microorganisms, organic fertilizers, etc., can solve the problems of high transportation costs, save treatment costs, have significant environmental and economic benefits, and low environmental risks Effect

Inactive Publication Date: 2018-05-25
QINGDAO KAILIER BIOTECH
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Problems solved by technology

(6) The application of liquid fertilizers also has certain limitations, such as relatively high transportation costs, liquid fertilizers used for soil fertilization require special storage and transportation equipment, and special application tools are also required.

Method used

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Examples

Experimental program
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Embodiment Construction

[0029] Choose from Bacillus megaterium, Candida lipolytica, and Pseudomonas. Use beef extract 3g, peptone 10g, NaCI5g, agar, water 1000mL to prepare Pseudomonas slant medium; use glucose 10g, NaCI0.3g, MgSO40.3g, MnSO40.03g, CaCO31.0g, (NH4)2SO40.5g , KCl0.3g, FeSO40.03g, lecithin 0.2g, yeast powder 0.5g, agar, water 1000mL, prepare Bacillus megaterium slant medium; utilize soybean sprouts 100g, glucose 20g, water 1000mL, agar, wash soybean sprouts , add water and boil for 30min. Filter with gauze, add agar to the filtrate, add sugar after heating to dissolve, stir to dissolve it, make up water to 1000mL, and prepare Candida tropicalis slant medium. Pseudomonas, Candida lipolytica, and Bacillus megaterium were respectively transferred to fresh slant medium, and cultured at 30°C for 24 hours to obtain activated strains. The activated strains were respectively put into the agar-free medium, the inoculum size was 2%, and they were all cultured at 30° C. for 18 hours, and the sp...

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PUM

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Abstract

The invention relates to a method for producing liquid organic bacterial manure by using kitchen waste liquid. The method comprises the steps: bacillus megaterium, candida lipolytica and pseudomonas are selected, a pseudomonas slant culture medium, a bacillus megaterium slant culture medium, and a candida tropicalis slant culture medium are prepared, pseudomonas, candida lipolytica, and bacillus megaterium are correspondingly transferred to a fresh slant culture medium and incubate at 30 DEG C for 24 h for activation, activated bacteria are correspondingly placed in an agar-removing medium andare cultured at 30 DEG C for 18 hours, inoculation amount is 2%, table rotating speed is 180 rpm, and activated bacterial liquid is obtained. The activated pseudomonas, bacillus megaterium, and candida lipolytica are inoculated into the kitchen waste liquid in a ratio of 1 to 1 to 1, the inoculation amount is 2%. 60% of livestock and poultry excrement materials are added to prepare culture enlarging bacterial liquid. The compound bacterial liquid is fermented at 30 DEG C for 72 hours to obtain the liquid organic bacterial manure.

Description

technical field [0001] The invention relates to the field of regeneration and utilization of waste resources, in particular to a method for producing liquid organic bacterial fertilizer by using kitchen waste liquid, which includes a strain expansion system. Background technique [0002] The COD of kitchen waste liquid can reach up to 100,000 mg / L, which belongs to high-concentration organic wastewater. If it is discharged into water bodies, it will cause serious eutrophication problems, and the cost of treating it to meet the discharge standards is very high. Therefore, using kitchen waste liquid rich in organic components as a nutrient solution to produce liquid bacterial fertilizer through microbial expansion can not only eliminate wastewater discharge, but also realize a high degree of resource utilization of waste liquid. Bacterial fertilizer is a common name for compound biological fertilizer, which is a biological fertilizer made by artificially cultivating certain be...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05F15/00C05F7/00C05F11/08C05F17/00C12N1/20C12N1/16C12R1/38C12R1/11C12R1/73
CPCC05F3/00C05F7/00C05G5/20C12N1/16C12N1/20C05F11/08C05F17/00Y02A40/20Y02W30/40
Inventor 范魏
Owner QINGDAO KAILIER BIOTECH
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