Antierythrite production method

A technology of erythritol and its production method, which is applied in the field of erythritol cultivation, can solve the problems of long fermentation period, unfavorable bacterial contamination, extraction rate of only 75%, unfavorable removal of impurities such as bacterial protein, etc., and shorten the fermentation process. cycle, improve clarity, and reduce production costs

Inactive Publication Date: 2008-11-12
QINGDAO LANGYATAI GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the current production method, the fermentation period is more than 150 hours, and the plate-and-frame filtration technology is used for the separation of the fermentation broth. Too long fermentation period is not conducive to the control o...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0008] Example 1: Using glucose as raw material and Candida lipolytica as the fermentation strain, cultured on a shaker and expanded cultivation of primary and secondary seeds, and finally fermented in a fermenter. At a tank temperature of 25°C and a tank pressure of 0 MPa, dissolved oxygen 20% saturation, at C 6 h 12 o 6 ·H 2 The initial content of O is 20%, the inorganic salt MgSO 4 ·7H 2 The O content is 0.04%, (NH 4 ) 2 SO 4 Content is 0.04%, KH 2 PO 4 Content is 0.10%, CuSO 4 Fermentation is carried out under the conditions of 0.04% yeast extract content and 0.80% yeast extract content. The fermentation period is 100 hours. The fermentation broth uses ceramic membranes to remove impurities such as bacterial protein, and then uses sodium membranes imported from the United States to selectively pass erythritol. The erythritol is extracted, finally concentrated and crystallized, separated and dried to obtain the erythritol product. After testing, the sugar content...

Embodiment 2

[0009] Example 2: Using glucose as raw material and Candida lipolytica as the fermentation strain, cultured on a shaker and expanded cultivation of primary and secondary seeds, and finally fermented in a fermenter, dissolved at a tank temperature of 30°C and a tank pressure of 0.05MPa Oxygen 50% saturation at C 6 h 12 o 6 ·H 2 The initial content of O is 30%, the inorganic salt MgSO 4 ·7H 2 The O content is 0.06%, (NH 4 ) 2 SO 4 Content is 0.05%, KH 2 PO 4 Content is 0.13%, CuSO 4 Fermentation is carried out under the conditions of 0.05% yeast extract content and 0.86% yeast extract content. The fermentation period is 110 hours. The fermentation broth uses ceramic membranes to remove impurities such as bacterial protein, and then uses sodium membranes imported from the United States to selectively pass erythritol. The erythritol is extracted, finally concentrated and crystallized, separated and dried to obtain the erythritol product. After testing, the sugar content...

Embodiment 3

[0010] Example 3: Using glucose as the raw material and Candida lipolytica as the fermentation strain, cultured on a shaker and expanded cultivation of primary and secondary seeds, and finally fermented in a fermenter, dissolved at a tank temperature of 35°C and a tank pressure of 0.1MPa Oxygen 80% saturation at C 6 h 12 o 6 ·H 2 The initial content of O is 40%, the inorganic salt MgSO 4 ·7H 2 O content is 0.08%, (NH 4 ) 2 SO 4 Content is 0.06%, KH 2 PO 4 Content is 0.15%, CuSO 4 Fermentation is carried out under the conditions of 0.06% yeast extract content and 0.90% yeast extract content. The fermentation period is 120 hours. The fermentation broth uses ceramic membranes to remove impurities such as bacterial protein, and then uses sodium membranes imported from the United States to selectively pass erythritol. The erythritol is extracted, finally concentrated and crystallized, separated and dried to obtain the erythritol product. After testing, the sugar content ...

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Abstract

The invention discloses a method for producing erythritol. Glucose is taken as raw material, candidalipolytica is taken as a fermentation strain, shaking culture as well as first-stage and second-stage inoculum development are performed, then the fermentation in a fermentation tank is carried out, mycoprotein impurities in zymotic fluid are removed by a ceramic membrane after the fermentation is finished, then a sodium membrane is used to selectively pass through the erythritol so as to extract the erythritol, and finally the erythritol product is obtained through condensation crystallization and separation drying. The method has the advantages of short incubation period, high extraction yield, low production cost and so on.

Description

technical field [0001] The invention relates to a cultivation method of erythritol. Background technique [0002] Erythritol is a four-carbon polyol with both sweetness and no calories. It is widely found in seaweed, mushrooms, melons, grapes, pears and fermented foods; at the same time, it also exists in the tissues and body fluids of humans and animals. Erythritol has the advantages of low energy value, high tolerance, no side effects, edible for diabetics, non-cariogenic, etc., and is extremely suitable for use in functional foods. The preparation method of erythritol is mainly microbial fermentation method, and its industrial production is based on starch as raw material, which is fermented by hypertonic yeast. In the current production method, the fermentation period is more than 150 hours, and the plate-and-frame filtration technology is used for the separation of the fermentation broth. Too long fermentation period is not conducive to the control of bacterial contam...

Claims

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Application Information

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IPC IPC(8): C12P19/02C12R1/73
Inventor 李悦明张希铭徐建春孙慧彬刘同照陈金凤曲文强
Owner QINGDAO LANGYATAI GRP
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