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Method for increasing erythritol conversion rate through adding mixed magnesium salt

A technology of erythritol and conversion rate, applied in the field of microbial fermentation production, can solve the problems of low conversion rate and the like, and achieve the effects of fewer processes, simple operation and good economic benefits

Active Publication Date: 2020-02-18
BAOLINGBAO BIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Chinese patent document CN103290068A discloses a method for producing erythritol by Aureobasidium pullulans resistant to hyperosmosis, adopting xylose as the fermentation medium of main carbon source to produce erythritol, but its conversion rate is low, only 25-42%
But the conversion rate of this method is also relatively low, only 30~40%

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A method for improving erythritol conversion rate by adding mixed magnesium salts, comprising steps as follows:

[0028] (1) Cultivation of Candida lipolytica seed liquid: Under a sterile environment, use an inoculation loop to hook 2 loops of Candida lipolytica and transfer it to the shake flask medium, at 30°C, 105r / min Shake flask culture under conditions for 20 hours, take the Candida lipolytica shake flask culture fluid and inoculate it into the primary seed medium, the inoculum amount is 1% of the volume of the primary seed medium, at 30±1°C, 180r / Cultivate for 14 hours under the condition of 1 min; then insert the primary seed liquid into the secondary seed medium with 10% inoculation amount and cultivate it for 7 hours at 30°C and 180 r / min to obtain Candida lipolytica seeds liquid;

[0029] (2) Fermentation culture: the Candida lipolytica seed liquid that will prepare is inserted in the fermentor that fermentation medium is housed by 9% by the inoculum size, ...

Embodiment 2

[0035] A method for improving erythritol conversion rate by adding mixed magnesium salts, comprising steps as follows:

[0036] (1) Cultivation of Candida lipolytica seed liquid: Under a sterile environment, use an inoculation loop to hook 1 ring of Candida lipolytica and transfer it to the shake flask medium, and transfer it to the shake flask medium, at 32°C, 105r / min Shake flask culture under the conditions for 24 hours, take Candida lipolytica shake flask culture fluid and inoculate it into the first-level seed medium, the inoculation amount is 1% of the volume of the first-level seed medium, at 30±1°C, 200r / Cultivate for 20 hours under the condition of 1 min; then insert the primary seed liquid into the secondary seed medium with 9% inoculum amount, and cultivate for 12 hours at 32°C and 180-200 r / min to obtain lipolytic candida Yeast seed liquid;

[0037](2) Fermentation culture: the prepared Candida lipolytica seed liquid is inserted in the fermenter that the fermenta...

Embodiment 3

[0043] A method for improving erythritol conversion rate by adding mixed magnesium salts, comprising steps as follows:

[0044] (1) Cultivation of Candida lipolytica seed liquid: Under a sterile environment, use an inoculation loop to hook 2 rings of Candida lipolytica and transfer it to the shake flask medium, at 31°C, 105 rpm Shake flask culture was carried out under certain conditions, and then the shake flask culture solution of Candida lipolytica was inoculated into the primary seed medium at 31°C, and the stirring speed was 190r / min. 1% of the base mass; then the primary seed liquid is inserted into the secondary seed medium at 31° C. and cultivated for 10 h under the condition of 190 r / min with a 10% inoculation amount to obtain the Candida lipolytica seed liquid ;

[0045] (2) Fermentation culture: the Candida lipolytica seed liquid that will prepare is inserted in the fermenter that fermentation medium is housed by 9% by the inoculum size, at stirring speed 90 revs / m...

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PUM

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Abstract

The invention relates to a method for increasing the erythritol conversion rate through adding mixed magnesium salt. The method comprises the steps of firstly culturing candida lipolytica didd. et lodd. strain liquid, and then performing fermentation culture on the candida lipolytica didd. et lodd. strain liquid so as to obtain the erythritol, wherein fermentation culture mediums include components of 0.3-0.35kg / L of glucose, 4-7g / L of organic nitrogen sources and 5-6g / L of inorganic salt. According to the method disclosed by the invention, part of raw materials of the fermentation culture mediums are replaced, addition of single magnesium salt is changed into addition of mixed magnesium salt, the proportion of the mixed magnesium salt is adjusted, and the fermentation culture mediums areoptimized, so that the strain metabolic pathway more facilitates generation of the erythritol, and the erythritol conversion rate can be effectively increased.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation production, in particular to a method for increasing the conversion rate of erythritol by adding mixed magnesium salts. Background technique [0002] Erythritol is widely distributed in nature and is a natural sugar. Erythritol is similar to sucrose in sweetness, but it is different from sucrose. Erythritol tastes cool and has good processing properties. It can be widely used in various foods and beverages such as candy, beverage, jelly and jelly-type beverage, oral health care products, etc. Like other sugar alcohols, erythritol does not cause dental caries. The reason is that there is no enzyme system to metabolize erythritol in the human body, so when the small intestine absorbs it into the blood, it cannot be metabolized, and almost all of it is excreted with the urine, which is suitable for use as a sweetener for diabetics. It can also avoid the side effects of bloating, bo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/18C12N1/16C12R1/73
CPCC12N1/16C12P7/18
Inventor 李克文熊小兰栾庆民张莉贾慧慧张倩李珍珍任晓洁李新华尹郑
Owner BAOLINGBAO BIOLOGY
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