Fermentation method for producing erythritol

Abstract

The invention discloses a fermentation method for producing erythritol. The fermentation method comprises the following steps that candida lipolytica strains are activated and subjected to shake culture and first-grade and second-grade seed enlarged culture; citric acid is dissolved with water, ammonium hydroxide is added, and ammonium citrate is prepared; an organic nitrogen source, inorganic salt and water are added into a nutritive salt tank for dissolving and evenly stirred; ammonium citrate is injected into the nutritive salt tank and mixed with nutritive salt, and the mixture is evenly stirred and heated through steam for sterilization; a glucose solution is injected into a fermentation tank and heated through steam for sterilization; a second-grade seed solution and nutritive salt mixed solution is injected into the fermentation tank and is evenly mixed with the sterilized glucose solution; the stirring rotating speed of the fermentation tank is set, compressed air is introduced, the temperature is well controlled, and fermentation is started; then, the stirring rotating speed is decreased, the air introducing amount is increased, and the fermentation temperature is well controlled; fermentation is ended with glucose is smaller than or equal to 0.1 g / 100 ml. By means of the fermentation method, the problems that an existing method is low in conversion rate of erythritol and long in production period are solved, and the fermentation method has the advantages that the operation cost is low, the fermentation period is short, and the yield of erythritol is high.

Description

technical field [0001] The invention relates to the technical field of production of erythritol, in particular to a fermentation method for producing erythritol. Background technique [0002] Erythritol is a natural, zero-calorie, filling sweetener that can replace sucrose. It has a clear sweetness similar to sucrose. Like other sugar alcohols, erythritol does not cause dental caries. It is slightly better than xylitol. The reason is that there is no enzyme system to metabolize erythritol in the human body, so when it is absorbed into the blood by the small intestine, it cannot be metabolized, and almost all of it is excreted with urine. It can avoid the side effects of bloating, borborygmus and diarrhea caused by excessive amounts like other sugar alcohols. It can be applied to all kinds of people, especially diabetics. Based on the functions and nutritional effects of erythritol, it can be widely used in various foods and beverages such as candies, beverages, jelly and j...

AI Technical Summary

Problems solved by technology

At present, in the production process of erythritol, the control of fermentation conditions is relatively simple, and real-time adjustment cannot be made according to the growth and metabolism characteristics of hypertonic yeast, so as to achieve the optimal growth and metabolism environment of hypertonic yeast. The conversion rate reaches the maximum and the production cycle reaches the shortest