Fermentation method for producing erythritol

A fermentation method and erythritol technology are applied in the field of fermentation for producing erythritol, which can solve the problems of high erythritol conversion rate, single fermentation condition, short production cycle and the like, and achieve low operating cost and fermentation cycle. Short, yield-enhancing effect

Inactive Publication Date: 2016-01-20
FUTASTE PHARM CO LTD
View PDF3 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, in the production process of erythritol, the control of fermentation conditions is relatively simple, and real-time adjustment cannot be made according to the growth and metabolism characteristics of hypertonic yeast, so as to achieve the optimal growth and metabolism environment of hypertonic yeast. The conversion rate reaches the maximum and the production cycle reaches the shortest

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The steps of the fermentation method are as follows:

[0025] (1) Candida lipolytica strains are activated, cultured on a shaking table, inoculated into a first-level seed tank for expanded cultivation, and after the number of cells reaches a certain number, inoculated into a second-level seed tank for expanded cultivation; The seed liquid is 8% of the secondary seed liquid quality in the secondary seed tank, and the secondary seed liquid is 8% of the fermentation liquid quality.

[0026] (2) Dissolve citric acid in water, add a certain amount of ammonia water to adjust the pH value to 7 to make weakly alkaline ammonium citrate; adjust the ammonium citrate to this range to make the pH value of the fermentation broth reach 5-6, and this pH value range It is the optimum pH value for the growth of Candida lipolytica, and after the growth of Candida lipolytica, the pH value can just drop to the optimum pH range of 3-4 suitable for the metabolism of Candida lipolytica;

[0...

Embodiment 2

[0035] The steps of the fermentation method are as follows:

[0036] (1) Candida lipolytica strains are activated, cultured on a shaking table, inoculated into a first-level seed tank for expanded cultivation, and after the number of cells reaches a certain number, inoculated into a second-level seed tank for expanded cultivation; The seed liquid is 10% of the quality of the secondary seed liquid in the secondary seed tank, and the secondary seed liquid is 10% of the quality of the fermentation liquid.

[0037] (2) Dissolve citric acid in water, add a certain amount of ammonia water to adjust the pH value to 9 to make weakly alkaline ammonium citrate; adjust the ammonium citrate to this range to make the pH value of the fermentation broth to 5-6, and this pH value range It is the optimum pH value for the growth of Candida lipolytica, and after the growth of Candida lipolytica, the pH value can just drop to the optimum pH range of 3-4 suitable for the metabolism of Candida lipo...

Embodiment 3

[0046] The steps of the fermentation method are as follows:

[0047] (1) Candida lipolytica strains are activated, cultured on a shaking table, inoculated into a first-level seed tank for expanded cultivation, and after the number of cells reaches a certain number, inoculated into a second-level seed tank for expanded cultivation; The seed liquid is 9% of the secondary seed liquid quality in the secondary seed tank, and the secondary seed liquid is 9% of the fermentation liquid quality.

[0048](2) Dissolving citric acid with water, adding a certain amount of ammonia water to adjust the pH value to 8 to obtain weakly alkaline ammonium citrate; adjusting the ammonium citrate to this range can make the pH value of the fermentation broth to 5-6, and this pH value range It is the optimum pH value for the growth of Candida lipolytica, and then the pH value after the growth of Candida lipolytica can just drop to the optimum pH value range of 3-4 suitable for the metabolism of Candid...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a fermentation method for producing erythritol. The fermentation method comprises the following steps that candida lipolytica strains are activated and subjected to shake culture and first-grade and second-grade seed enlarged culture; citric acid is dissolved with water, ammonium hydroxide is added, and ammonium citrate is prepared; an organic nitrogen source, inorganic salt and water are added into a nutritive salt tank for dissolving and evenly stirred; ammonium citrate is injected into the nutritive salt tank and mixed with nutritive salt, and the mixture is evenly stirred and heated through steam for sterilization; a glucose solution is injected into a fermentation tank and heated through steam for sterilization; a second-grade seed solution and nutritive salt mixed solution is injected into the fermentation tank and is evenly mixed with the sterilized glucose solution; the stirring rotating speed of the fermentation tank is set, compressed air is introduced, the temperature is well controlled, and fermentation is started; then, the stirring rotating speed is decreased, the air introducing amount is increased, and the fermentation temperature is well controlled; fermentation is ended with glucose is smaller than or equal to 0.1 g / 100 ml. By means of the fermentation method, the problems that an existing method is low in conversion rate of erythritol and long in production period are solved, and the fermentation method has the advantages that the operation cost is low, the fermentation period is short, and the yield of erythritol is high.

Description

technical field [0001] The invention relates to the technical field of production of erythritol, in particular to a fermentation method for producing erythritol. Background technique [0002] Erythritol is a natural, zero-calorie, filling sweetener that can replace sucrose. It has a clear sweetness similar to sucrose. Like other sugar alcohols, erythritol does not cause dental caries. It is slightly better than xylitol. The reason is that there is no enzyme system to metabolize erythritol in the human body, so when it is absorbed into the blood by the small intestine, it cannot be metabolized, and almost all of it is excreted with urine. It can avoid the side effects of bloating, borborygmus and diarrhea caused by excessive amounts like other sugar alcohols. It can be applied to all kinds of people, especially diabetics. Based on the functions and nutritional effects of erythritol, it can be widely used in various foods and beverages such as candies, beverages, jelly and j...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12P7/18
Inventor 孙鲁邱学良曲廷云李金华刘海鹏秦海青袁敏
Owner FUTASTE PHARM CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap