Candida lipolytica and the erythritol producing process therewith

A technology of Candida lipolytica and erythritol, which is applied in the field of microbial fermentation, can solve the problems of unfavorable product purity and high cost of erythritol, and achieves improved extraction yield, reduced production cost, and reduced pollution and harmful wild impurities. The effect of bacterial risk

Active Publication Date: 2009-07-01
BEIJING REFINE BIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the cost of erythritol produced by using the fermentation technology of Bustle sp. yeast and glucose as carbon source is relatively high, and it still contains a small amount of ribitol, glycerol and pigments, which is unfavorable for improving the purity of the product.

Method used

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  • Candida lipolytica and the erythritol producing process therewith
  • Candida lipolytica and the erythritol producing process therewith

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] In a 5-ton fermenter, 3.5 tons of feed liquid containing culture medium are put into the medium, and the proportion of the culture medium is as follows: 900 kilograms of glucose, 21 kilograms of yeast extract, 3 kilograms of potassium dihydrogen phosphate, 1.5 kilograms of magnesium sulfate, and 20 kilograms of ammonium sulfate . The inoculum amount is 10%, the stirring speed is 200r.p.m, and the air volume is 30m 3 / h, dissolved oxygen 20%, tank pressure 0.5atm, temperature 30°C-31°C, fermentation time 132 hours, wherein, after 24 hours, the pH of the fermentation broth was controlled between 2.6-2.9, red Bacterol 15.4%, residual sugar less than 0.5%, sugar alcohol conversion rate 47%. Membrane filtration is performed on the fermented liquid, the solid bacteria residue is filtered off, the filtrate is decolorized with activated carbon, desalted through an ion exchange column, and the feed liquid is concentrated at 65°C under reduced pressure until the content of eryth...

Embodiment 2

[0052] In this example, relatively cheap corn steep liquor was used instead of yeast extract, and the components of the medium were simplified to reduce the cost of the fermentation medium. In a 5-ton fermenter, 3.5 tons of feed liquid containing a culture medium were dropped into, and the proportion of the culture medium was as follows: 1120 kilograms of glucose, 157.5 kilograms of corn steep liquor, and 35 grams of copper chloride. The inoculum amount is 10%, the stirring speed is 200r.p.m, and the air volume is 30m 3 / h, dissolved oxygen 20%, tank pressure 0.5 atm, temperature 30 ℃ ~ 31 ℃, fermentation time 130 hours, erythritol in the obtained fermentation broth is 15.3%, residual sugar is less than 0.5%, sugar alcohol conversion rate is 45.4 %. Membrane filtration is performed on the fermented liquid, the solid bacteria residue is filtered off, the filtrate is decolorized with activated carbon, desalted through an ion exchange column, and the feed liquid is concentrated ...

Embodiment 3

[0054] In this example, the recovered yeast is used to autolyze instead of commercial yeast extract, which can significantly reduce the cost of the medium, and at the same time reduce the discharge of waste water containing solid impurities, thereby improving the environment. Take fresh yeast slime (with a water content of 80%), adjust the pH to 6.5-7.5 with 1mol / l sodium hydroxide solution, incubate at 45-55°C for 24-48 hours, and recover the filtrate after filtration. In a 5-ton fermenter, 3.5 tons of feed liquid containing a culture medium are put into the feed, and the ratio of the culture medium is as follows: 1120 kilograms of glucose, 500 kilograms of yeast autolysate with a solid content of 7%, 1.05 kilograms of potassium dihydrogen phosphate, sulfuric acid Magnesium 0.7 kg, ammonia 3.5 kg, copper chloride 35 grams. Inoculation after sterilization, inoculum size 10%, stirring speed 200r.p.m, air volume 30m3 / h, dissolved oxygen 20%, tank pressure 0.5atm, temperature 30℃...

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Abstract

The present invention relates to microbial fermentation technology, and is especially Candida lipolytica and the erythritol producing process therewith. The erythritol producing process includes the following steps: 1. fermentation in glucose containing culture medium with Candida lipolytica for producing erythritol in high yield; and 2. separating and purifying erythritol from the fermented liquid.

Description

field of invention [0001] The invention relates to the field of microbial fermentation, in particular to Candida lipolytica with high yield of erythritol and a method for producing erythritol by using the yeast strain. Background technique [0002] Because erythritol is widely used in food, medicine, cosmetics, chemical industry, etc., the demand for it is increasing day by day. In history, people first isolated erythritol from plant sources such as algae and moss, but the content was low and extraction was difficult; then there was a chemical synthesis method to synthesize erythritol from 2-butene-1,4-butanediol Britol, but the cost is high, the conversion rate is low, and the separation and purification of the product is difficult to wait for industrial implementation; later, it basically turned to the method of yeast fermentation production to produce erythritol. [0003] Japanese Patent No. 4741549 discloses a method for fermenting and producing erythritol using Trichos...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/16C12P7/18C12R1/73
Inventor 裴疆森黄玲
Owner BEIJING REFINE BIOLOGY
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