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Method for preparing gamma-decalactone (GDL) from kitchen waste grease

A technology of waste oil and decanolactone, which is applied in the field of food-grade natural flavor γ-decalactone, can solve the problems of large differences in output, and achieve the effect of saving production costs and reducing costs

Active Publication Date: 2019-11-12
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is known that Yarrowia lipolytica (also known as Candida lipolytica) can secrete a large amount of lipase and esterase, which can decompose and utilize lipid substances for growth, but the enzyme activity and GDL production of different strains of Yarrowia lipolytica vary greatly Big, if using kitchen waste oil as a medium component for yeast culture and transformation to produce GDL, it is an economical and environmentally friendly method, and it is a way with great potential for production and application. There is no relevant report so far.

Method used

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  • Method for preparing gamma-decalactone (GDL) from kitchen waste grease
  • Method for preparing gamma-decalactone (GDL) from kitchen waste grease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 Degradation of oil and production of GDL by two strains of yeast

[0032] 1. Determination of the ability of different yeast strains to degrade esters.

[0033] Esters and lactones react with hydroxylamine under alkaline conditions to form hydroxamic acid, and hydroxamic acid complexes with Fe under acidic conditions to form a purple-red complex. 506 nm absorbs.

[0034] Through screening and verification of various strains, single colonies of Yarrowia lipolytica (Yarrowialipolytica) CICC 32187 and Candida lipolytica (Candida lipolytica) CICC 31223 were finally picked and inoculated in 5 mL of YPD liquid medium, shaken at 28 °C (200r / min) and cultured overnight; transfer the inoculation amount of 10% volume concentration to 30 mL of YPD liquid medium, culture at 28 ° C for 12 h with shaking (200 r / min), centrifuge at 6 000 r / min for 3 min, and buffer the bacterial cells with sorbitol washed twice, and adjust the cell concentration to OD 600 The bacterial s...

Embodiment 2

[0044] Example 2 Using the culture medium mixed with kitchen waste oil to carry out biofermentation of ricinoleic acid to produce gamma-decalactone

[0045] 1. Activation of strains: Inoculate Yarrowia lipolytica CICC 32187 and Candida lipolytica CICC 31223 into YPD medium respectively, culture at 28°C for 2 days for activation, and obtain activated single colonies respectively .

[0046] The quality composition of the YPD medium is as follows: 2% glucose, 2% peptone, 1% yeast powder, 2% agar, deionized water as a solvent, and natural pH. The medium was sterilized at 121 °C for 20 min.

[0047] 2. Seed culture: Pick 3 to 5 single colonies of the above-mentioned activated yeast and inoculate them in a 500mL Erlenmeyer flask containing 300mL seed medium under aseptic conditions, and cultivate them on a shaker at 28-30°C and 150rpm for 1 day , to obtain Yarrowia lipolytica seed liquid and Candida lipolytica seed liquid respectively. No contamination was found through microscop...

Embodiment 3

[0056] Embodiment 3 Utilizes the substratum mixed culture that contains kitchen waste grease to ferment castor oil to produce gamma-decalactone

[0057] Change the ricinoleic acid in step 4 of Example 2 to 80g / L castor oil, and change the kitchen waste oil mass concentration into 3% in the GDL transformation medium, and other operations are the same as in Example 1. The output of 1 liter of fermentation broth was 0.15g / L.

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Abstract

The invention discloses a method for preparing gamma-decalactone (GDL) from kitchen waste grease. A yarrowia lipolytica bacterium suspension and a candida lipolytica bacterium suspension are mixed andinoculated into a fermentation medium containing the kitchen waste grease to be cultured for 20-24 h at 28-30 DEG C, and fermentation liquor containing yarrowia lipolytica and candida lipolytica is obtained; and the fermentation liquor with the inoculation amount being 10% of the volume concentration is inoculated into a GDL conversion medium containing 60-80 g / L of ricinoleic acid or castor oiland subjected to shaking culture for 72 h at 28 DEG C, GDL conversion production is conducted, and the GDL is obtained. The kitchen waste grease is utilized as part of a carbon source, yeast strains are successfully cultured, and GDL conversion production is conducted; and the cost of the medium and a mixed fermentation method is lowered, the effect of turning waste into treasure is achieved, theGDL with the high additional value is produced, the kitchen waste grease is subjected to resource utilization, and the obvious economic and environment-friendly benefits are achieved.

Description

[0001] (1) Technical field [0002] The invention relates to a method of using kitchen waste oil to replace part of the carbon source in the complete culture medium, and using yeast mixed bacteria culture with strong ability to decompose oil to fully utilize the oil to obtain Yarrowia lipolytica containing more biomass, and then using lipolytic Yarrowia transforms ricinoleic acid into food-grade natural flavor γ-decalactone. [0003] (2) Background technology [0004] γ-decalactone (γ-decalactone, GDL) is a lactone flavor naturally present in strawberries, peaches and other fruits. It has a low aroma threshold and is internationally recognized as a safe food additive. Coconut, strawberry, peach and other spices are widely used in margarine, ice cream, soft drinks, candy, baked goods and seasonings, and the market demand is very large. With the increasing consumption of delicious food, beverages, etc., the demand for natural food flavoring additives is increasing. [0005] In ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P17/04C12R1/645C12R1/73
CPCC12P17/04
Inventor 朱廷恒王荣霞汪琨王渭霞
Owner ZHEJIANG UNIV OF TECH
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