Method for producing phycite by using corn meal

A technology of erythritol and corn flour, which is applied in the field of fermentation and production of erythritol, can solve the problems of non-recyclable waste bacteria, low resource utilization efficiency, and increased environmental protection pressure, so as to avoid repetitive waste water Effects of treatment and energy consumption, increased bioavailability, increased efficiency

Active Publication Date: 2011-08-17
山东保龄宝悦美康生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0019] From the perspective of energy consumption, each production process requires repeated energy-consuming operations such as drying and concentration, which consume a lot of energy.
From the perspective of environmental protection and wastewater treatment, each production process produces a large amount of industrial wastewater, which requires wastewater treatment before discharge, resulting in increased product costs and increased pressure on environmental protection
From the perspective of effective utilization of resources, each processing process will produce by-products and certain losses, and the overall resource utilization efficiency is not high
[0020] In addition, the existing sterilization process of erythritol production adopts plate-and-frame filtration and adding activated carbon for decolorization. The waste bacteria cannot be recycled, and the waste bacteria are discharged to the sewage treatment plant for biological treatment, resulting in a large amount of pollutant discharge

Method used

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  • Method for producing phycite by using corn meal

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0153] 1. Strain culture

[0154] Take 200g of degerminated corn flour and 2.5g of yeast cells, after mixing and extruding, add water to make up to 0.7L, put it into a 3L seed tank, raise the temperature of the feed liquid to 50°C in advance, stir, and adjust the pH value of the feed liquid to 5.8- 6.0, add 0.09mL of high-temperature-resistant amylase, the addition amount is 0.6L / t starch dry basis, heat up to 100°C for liquefaction by passing steam, and keep warm for 20min. Then lower the temperature to 50-55°C, adjust the pH to 4.5, add high-efficiency liquid glucoamylase or compound glucoamylase 0.09mL, add 0.6L / t starch dry basis, add protease 0.03g, stir at 50-55°C for 20 hours, and saccharify The liquid DE value is 95.3%.

[0155]Raise the temperature of the saccharification feed solution to 121°C for 20 minutes, then cool it down to 28-35°C, adjust the volume of the feed solution to 0.8L, adjust the pH to 5.5-6.5, insert Candida lipolytica CGMCC No.1431 strain for cult...

Embodiment 2

[0162] 1. Bacteria culture

[0163] Take 200g of degerminated corn flour and 2.5g of yeast cells, after extrusion treatment, add water to make the volume to 0.7L, put it into a 3L seed tank, raise the temperature of the feed liquid to 50°C in advance, stir, and adjust the pH value of the feed liquid to 5.8- 6.0, add 0.09mL of high-temperature-resistant amylase, the addition amount is 0.6L / t corn flour dry basis, heat up to 100°C for liquefaction by passing steam, and keep warm for 20min. Then lower the temperature to 50-55°C, adjust the pH to 4.0-4.5, add high-efficiency liquid glucoamylase or compound glucoamylase 0.09ml, add 0.6L / t corn flour dry basis, add protease 0.03g, stir and saccharify at 50-55°C After 20 hours, the DE value of the saccharification solution was 95.3%.

[0164] Raise the temperature of the saccharification feed liquid to 121°C for 20 minutes, cool down to 28-35°C, adjust the volume of the feed liquid to 0.8L, adjust the pH to 5.5-6.5, insert Candida l...

Embodiment 3

[0171] 1. Bacteria culture

[0172] Take 200g of degerminated corn flour, 2.5g of yeast cells, add water to make the volume to 0.7L, put it into a 3L seed tank, raise the temperature of the feed liquid to 50°C in advance, stir, adjust the pH value of the feed liquid to 5.8-6.0, and add high-temperature-resistant starch Enzyme 0.09mL, additive amount 0.6L / t dry basis of corn flour, feed liquid is directly passed into steam and heated to 100°C for liquefaction, and kept for 20min. Then lower the temperature to 50-55°C, adjust the pH to 4.5, add 0.09mL of high-efficiency liquid glucoamylase or compound glucoamylase, add 0.03g of protease, add 0.6L / t corn flour dry basis, stir and saccharify at 50-55°C for 20h, The DE value of the saccharification liquid reaches 94.3%.

[0173] Raise the temperature of the saccharification feed liquid to 121°C for 15 minutes, cool down to 28-35°C, adjust the volume of the feed liquid to 0.8, adjust the pH to 5.5-6.5, insert Candida lipolytica CGM...

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Abstract

The invention relates to a method for producing phycite by fermenting corn meal serving as a starting raw material with candida lipolytica, belonging to the field of microbial fermentation. The method comprises the following steps of: (1) mixing and extruding corn meal with separated and recycled yeast; (2) liquefying and saccharifying the material by undergoing an enzymatic hydrolysis reaction and fermenting phycite by inoculating fermentation seeds to obtain a phycite-containing fermentation liquid; and (3) degerming the fermentation liquid with a metal microfiltration membrane, separating the material from the liquid, and performing moisture evaporation concentration and crystallization on supernate by using a climbing film evaporator. The corn meal is directly taken as the raw material; and mixing, enzymatic hydrolysis and fermentation culturing are performed in the same fermentation tank, processes and devices for producing starch and glucose in the prior art are eliminated, repeated treatment of waste water produced in the production process and energy consumption are avoided, the flow is more economic and reasonable, and the resource utilization ratio can be effectively increased. By adopting a candida lipolytica strain with high phycite yield, screened with the method, the phycite yield can be increased by about 2 percent.

Description

technical field [0001] The invention belongs to the field of microbial fermentation, and in particular relates to a new method for fermenting and producing erythritol by using corn flour as a starting material. Background technique [0002] Erythritol is widely distributed in nature, such as fruits, mushrooms, lichens, etc. In addition, it also exists in fermented foods and mammals, and is a natural sugar. Industrially, erythritol is a white crystal obtained by fermentation of glucose. It has a sweet taste, 70%-80% of the sweetness of sucrose, and its calories are only 0.2kcal / g. Erythritol is a four-carbon sugar alcohol with a melting point of 118-122°C, a cracking temperature of 329°C, and a molecular formula of CH 4 o 4 h 10 , the chemical structure is: [0003] [0004] Erythritol has unique nutritional properties, has relatively high digestibility, and is easily absorbed by the small intestine; erythritol does not affect blood sugar and insulin levels, so it is ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/18C12R1/74
Inventor 刘宗利王乃强栾庆民李克文袁卫涛熊晓兰张莉腾慧
Owner 山东保龄宝悦美康生物科技有限公司
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