Method for producing phycite by using corn meal

A technology of erythritol and corn flour, which is applied in the field of fermentation and production of erythritol, can solve the problems that waste bacteria cannot be recycled, the pressure of environmental protection is increased, and the resource utilization efficiency is not high, so as to avoid repeated wastewater treatment and energy consumption, improve efficiency, and improve the effect of bioavailability

Active Publication Date: 2011-11-30
山东保龄宝悦美康生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0019] From the perspective of energy consumption, each production process requires repeated energy-consuming operations such as drying and concentration, which consume a lot of energy.
From the perspective of environmental protection and wastewater treatment, each production process produces a large amount of industrial wastewater, which requires wastewater treatment before discharge, resulting in increased product costs and increased pressure on environmental protection
From the perspective of effective utilization of resources, each processing process will produce by-products and certain losses, and the overall resource utilization efficiency is not high
[0020] In addition, the existing sterilization process of erythritol production adopts plate-and-frame filtration and adding activated carbon for decolorization. The waste bacteria cannot be recycled, and the waste bacteria are discharged to the sewage treatment plant for biological treatment, resulting in a large amount of pollutant discharge

Method used

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  • Method for producing phycite by using corn meal

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0153] 1. Bacteria culture

[0154]Take 200g of degerminated corn flour and 2.5g of yeast cells. After mixing and extruding, add water to make the volume to 0.7L, and then put it into a 3L seed tank. The feed liquid is preheated to 50°C, stirred, and the pH value of the feed liquid is adjusted to 5.8- 6.0, add 0.09mL of high temperature resistant amylase, the addition amount is 0.6L / t starch dry base, pass steam to heat up at 100°C for liquefaction, and keep the temperature for 20min. Then cool down to 50-55°C, adjust the pH to 4.5, add 0.09mL of high-efficiency liquid saccharification enzyme or compound saccharification enzyme, add 0.6L / t starch dry base, add 0.03g of protease, stir and keep at 50-55°C for 20h, saccharification Liquid DE value 95.3%.

[0155] The saccharified feed solution was heated to 121°C for sterilization for 20min, cooled to 28-35°C, the volume of the feed solution was 0.8L, the pH was adjusted to 5.5-6.5, and cultured with Candida lipolytica CGMCC No....

Embodiment 2

[0162] 1. Bacterial culture

[0163] Take 200g of degerminated corn flour and 2.5g of yeast cells, extrude and extrude, add water to make the volume to 0.7L, put it into a 3L seed tank, warm the feed liquid to 50°C in advance, stir, and adjust the pH value of the feed liquid to 5.8- 6.0, add 0.09mL of high temperature resistant amylase, the amount of addition is 0.6L / t corn flour dry basis, pass steam to heat up at 100°C to liquefy, and keep the temperature for 20min. Then cool down to 50-55℃, adjust pH to 4.0-4.5, add 0.09ml high-efficiency liquid saccharification enzyme or compound saccharification enzyme, add 0.6L / t corn flour dry base, add protease 0.03g, stir and keep saccharification at 50-55℃ 20h, the DE value of the saccharification solution was 95.3%.

[0164] The saccharified feed solution was heated to 121°C for sterilization for 20min, cooled to 28-35°C, the volume of the feed solution was 0.8L, the pH was adjusted to 5.5-6.5, and the cultured with Candida lipolyt...

Embodiment 3

[0171] 1. Bacteria culture

[0172] Take 200g of degerminated corn flour, 2.5g of yeast cells, add water to make the volume to 0.7L, connect to a 3L seed tank, pre-heat the feed liquid to 50°C, stir, adjust the pH value of the feed liquid to 5.8-6.0, add high temperature resistant starch The enzyme is 0.09mL, the addition amount is 0.6L / t corn flour dry basis, the feed liquid is directly passed into the steam to heat up at 100°C to liquefy, and the temperature is kept for 20min. Then cool down to 50-55 ℃, adjust pH to 4.5, add 0.09 mL of high-efficiency liquid saccharification enzyme or complex saccharification enzyme, add 0.03 g of protease, add 0.6 L / t corn flour dry basis, stir and saccharify at 50-55 ℃ for 20 hours, The DE value of the saccharification solution reached 94.3%.

[0173] The saccharified feed solution was heated to 121°C for sterilization for 15min, cooled to 28-35°C, the volume of the feed solution was 0.8, the pH was adjusted to 5.5-6.5, and cultured with ...

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Abstract

The invention relates to a method for producing phycite by fermenting corn meal serving as a starting raw material with candida lipolytica, belonging to the field of microbial fermentation. The method comprises the following steps of: (1) mixing and extruding corn meal with separated and recycled yeast; (2) liquefying and saccharifying the material by undergoing an enzymatic hydrolysis reaction and fermenting phycite by inoculating fermentation seeds to obtain a phycite-containing fermentation liquid; and (3) degerming the fermentation liquid with a metal microfiltration membrane, separating the material from the liquid, and performing moisture evaporation concentration and crystallization on supernate by using a climbing film evaporator. The corn meal is directly taken as the raw material; and mixing, enzymatic hydrolysis and fermentation culturing are performed in the same fermentation tank, processes and devices for producing starch and glucose in the prior art are eliminated, repeated treatment of waste water produced in the production process and energy consumption are avoided, the flow is more economic and reasonable, and the resource utilization ratio can be effectively increased. By adopting a candida lipolytica strain with high phycite yield, screened with the method, the phycite yield can be increased by about 2 percent.

Description

technical field [0001] The invention belongs to the field of microbial fermentation, and in particular relates to a new method for fermenting and producing erythritol by using corn flour as a starting material. Background technique [0002] Erythritol is widely distributed in nature, such as fruits, mushrooms, lichens, etc. In addition, it also exists in fermented foods and mammals, and is a natural sugar. Industrially, erythritol is a white crystal obtained by fermentation of glucose. It has a sweet taste, 70%-80% of the sweetness of sucrose, and its calories are only 0.2kcal / g. Erythritol is a four-carbon sugar alcohol with a melting point of 118-122°C, a cracking temperature of 329°C, and a molecular formula of CH 4 o 4 h 10 , the chemical structure is: [0003] [0004] Erythritol has unique nutritional properties, has relatively high digestibility, and is easily absorbed by the small intestine; erythritol does not affect blood sugar and insulin levels, so it is ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/18C12R1/74
Inventor 刘宗利王乃强栾庆民李克文袁卫涛熊晓兰张莉腾慧
Owner 山东保龄宝悦美康生物科技有限公司
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