Method for improving oil sludge separating and processing efficiency and bacterial strain used for method

A treatment efficiency and oil sludge separation technology, applied in the field of improving oil sludge separation treatment efficiency, bacterial strains, can solve problems such as unsuitable oil sludge separation treatment

Active Publication Date: 2013-12-18
JIANGSU BODA ENVIRONMENTAL PROTECTION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the oil-degrading bacteria screened according to a specific location and the established oil sludge bi

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The sewage sludge near the hotel in Yixing City was selected to be screened by the following methods:

[0031] 1.1 Strain enrichment

[0032] Add a certain amount of oil sludge (2%) to the glucose medium in the Erlenmeyer flask, put it in a shaker, and culture it at 30° C. for 3 days to obtain a strain enrichment solution. The formula of glucose medium is: sodium chloride 1g, calcium chloride 0.5g, dipotassium hydrogen phosphate 0.5g, potassium dihydrogen phosphate 0.5g, ferric chloride 0.02g, ammonium nitrate 1g, magnesium sulfate heptahydrate, 1g yeast extract , 2g glucose, 1L water, pH 7.0-7.2; sterilize at 110°C for 20min.

[0033] 1.2 strain screening

[0034] After diluting the enrichment solution, streak and separate on the glucose solid medium plate (2% agar is added to the glucose medium), culture at 30°C for 24 hours, pick a single colony that grows well on the plate and streak Inoculate on an alkane plate, culture at 30°C for 48 hours, pick out the two lar...

Embodiment 2

[0036] Biological and biochemical tests were carried out on the Y-57 strain. The yeast YPD agar was cultured on the plate (30°C) for 3 days, and the colony could grow to 1-2 mm, and the color was white to cream. The initial colony was smooth, and after prolonged culture, the colony appeared wrinkled. When growing on solid or liquid medium, the cell shape is round or oval, and sometimes the cells are elongated and grow like false filaments, and the cell size is about 4-6×6-8 microns.

[0037] The yeast was identified as Candida lipolytica with the Biolog Microorganism Automatic Analyzer produced by Biolog Company of the United States.

[0038]Biological and biochemical tests were carried out on the P-101 strain. The bacterium was cultured aerobically on a nutrient agar plate for 24 hours. The bacteria grew vigorously, and the colonies were smooth, moist, translucent and light yellow colonies, and produced a strong smell. The Gram staining was negative bacillus , extremely many...

Embodiment 3

[0042] (1) Take the oil sludge sample from the settling tank of Huabei Oilfield, and analyze the processed oil sludge: According to the "Agricultural Sludge Monitoring and Analysis Method", the oil content is determined to be 10%, and the ion method is used for heavy metals. The heavy metal indicators are all less than the pollution in the agricultural sludge According to the substance control standard (GB4284-84), the total nitrogen in the oil sludge is determined to be 35mg / L by basic potassium persulfate, and the total phosphorus in the oil sludge is determined to be 12mg / L by ammonium molybdate spectrophotometry.

[0043] (2) Candida lipolytica Y-57 (CGMCC NO.5790) and Pseudomonas putida P-10 (CGMCC NO.5801) were cultured in LB medium at 30°C, 200rpm shaker for 24 hours, Then inoculate into the fermentation medium according to the inoculation amount of 5%, and cultivate for 36 hours at 30°C, 200rpm stirring, air volume 1:0.4-0.5, and tank pressure 0.05mpa.

[0044] Ferment...

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Abstract

The invention belongs to the environment-friendly field, and discloses a method for improving oil sludge separating and processing efficiency and a bacterial strain used for the method. The method for improving the oil sludge separating and processing efficiency comprises the following steps: (1), sampling and analyzing processed oil sludge; (2), determining microorganism nutrients to be added according to components and content of a nitrogen element and a phosphor element in the processed oil sludge; (3), adding a mixed culture of candida lipolytica Y-57 and pseudomonas putida P-101 and the microorganism nutrients into the to-be-processed oil sludge, stirring, ventilating and culturing for over 8 hours under the condition with pH of 6-10 under a temperature of 8 DEG C-45 DEG C to separate oil and mud in the oil sludge. According to the invention, a microorganism treatment method is adopted for separating the oil and the mud in the oil sludge to achieve an effect of an oil resource recovery rate; moreover, a chemical agent which generates influence to the environment is not added, so that secondary pollution is not generated. Resourceful treatment is carried out on oil-containing mud to obtain a certain economic benefit, and therefore, the method is an economical and effective oil sludge treating technology.

Description

technical field [0001] The invention belongs to the field of environmental protection, and relates to a method for improving the efficiency of oil sludge separation and treatment and a bacterial strain used therein. Background technique [0002] In the process of oil exploitation, storage and transportation, and refining and processing, some sludge with high oil content is often produced. If these sludge is piled up randomly, it will become an important source of pollution in the oil field and the surrounding environment. After oil enters the soil, it is difficult to remove, which not only destroys the soil structure, but also causes harm to the soil's own microorganisms and soil plant ecosystem. [0003] The composition of oily sludge is extremely complex, generally composed of oil-in-water, water-in-oil and suspended solid impurities, containing a large amount of aged crude oil, wax, asphaltene, colloid, suspended solids, bacteria, salts, acid gases, corrosion products et...

Claims

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Application Information

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IPC IPC(8): C02F11/02C12N1/16C12N1/20C12R1/73C12R1/40
CPCY02W10/20
Inventor 杜郭君吴倩李玉祥黄维杰
Owner JIANGSU BODA ENVIRONMENTAL PROTECTION
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