Preparation method of sea-buckthorn seed polypeptide used for sobering up from drunkenness

A technique for seabuckthorn seeds and sobering up, which is applied in the field of preparation of seabuckthorn seed polypeptides, can solve problems such as no research report on seabuckthorn seed dregs sobering polypeptides, and achieve the effect of high polypeptide yield and easy operation

Active Publication Date: 2015-11-18
一阳生集团有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no research report on the enzymatic pr

Method used

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  • Preparation method of sea-buckthorn seed polypeptide used for sobering up from drunkenness
  • Preparation method of sea-buckthorn seed polypeptide used for sobering up from drunkenness
  • Preparation method of sea-buckthorn seed polypeptide used for sobering up from drunkenness

Examples

Experimental program
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Effect test

Embodiment 1

[0014] Take 50g of pulverized defatted seabuckthorn seed dregs (26.25% protein content), add 800ml of distilled water, place it at room temperature for 10 hours, adjust the pH to 6.5, add 0.06g of papain and hydrolyze it at 60°C for 2.5h, after the enzyme is inactivated The enzymolysis solution was centrifuged at 5000r / min to obtain the supernatant, which was concentrated until the polypeptide content reached 1.5% for later use. After microfiltration, the enzymatic hydrolyzate of seabuckthorn seeds was used to determine the activation rate of alcohol dehydrogenase by Waller-Hoch method. After determination, when the polypeptide content is 15.8mg / mL, the activation rate of the polypeptide solution to alcohol dehydrogenase is 30.14%. Two components with molecular mass Mw>3000Da and Mw3000Da had no activation rate of alcohol dehydrogenase, and the component with Mw<3000Da had no effect on ethanol dehydrogenase The activation rate of hydrogenase was 57.41%, significantly higher t...

Embodiment 2

[0016] Take 50g of crushed defatted seabuckthorn seed dregs, add 600ml of distilled water, let it stand at room temperature for 8 hours, adjust the pH to 7.0, add 0.07g of papain and hydrolyze it at 50°C for 4.5h, inactivate the enzymatic solution at 5000r / The supernatant was collected by centrifugation for 1 min, and concentrated until the peptide content reached 1.5%. After microfiltration of the enzymatic solution, select ultrafiltration membranes and nanofiltration membranes with molecular weight cut-offs of 3000Da, 2000Da and 500Da to separate and obtain 4 components, namely molecular weight Mw>3000Da, Mw between 2000 and 3000Da, and Mw between 500 and 500Da. 2000Da, Mw<500Da. After the 4 kinds of separation liquid and the original enzymatic hydrolysis liquid were evaporated and concentrated to the same polypeptide content, the activation rate of different samples to alcohol dehydrogenase was determined by Waller-Hoch method. Gel chromatography was used to analyze the m...

Embodiment 3

[0023] Take 50g of pulverized defatted seabuckthorn seed dregs, add 1000ml of distilled water, let it stand at room temperature for 10 hours, adjust the pH to 6.0, add 0.09g of papain and hydrolyze it at 55°C for 3.5h, and inactivate the enzymatic solution at 5000r / The supernatant was collected by centrifugation for 1 min, concentrated until the polypeptide content reached 1.5%, and then separated and purified by an ultrafiltration membrane with a molecular weight cut-off of 3000Da to obtain a seabuckthorn seed polypeptide solution with a molecular weight less than 3000Da, which was concentrated until the polypeptide content reached 4% for later use.

[0024] The anti-drunk test procedure is to pour the polypeptide sample first and then pour the drinking concentration at 56° of liquor. 40 male mice (half male and half male) were randomly divided into 4 groups: model group; low, medium and high dose (100, 200, 400 mg / kg·bw) groups of seabuckthorn polypeptide. The model group ...

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Abstract

The invention provides a preparation method of sea-buckthorn seed polypeptide used for sobering up from drunkenness. The preparation method specifically includes: taking skim sea-buckthorn seed residue as a raw material; adding papain, and hydrolyzing under a most suitable condition; using an ultrafiltration membrane of 3000Da in molecular weight cut-off to separate enzymatic hydrolysate to obtain a sea-buckthorn seed sobering-up polypeptide solution smaller than 3000Da in molecular weight. In-vitro experiments show that the polypeptide solution has obvious activating effect on alcohol dehydrogenase, and activity of polypeptide from 500Da to 2000Da is highest; the polypeptide can promote decomposition and metabolism of in-vivo alcohol to realize sobering up from drunkenness by enhancing activity of alcohol dehydrogenase. Animal experiments show that the polypeptide can play a role in preventing drunkenness and sobering up from drunkenness if being taken in by 200mg/kg.bw and can be used as a raw material of sobering-up and healthcare products.

Description

technical field [0001] The invention relates to the preparation of seabuckthorn active polypeptides, in particular to a preparation method of seabuckthorn seed polypeptides for sobering up alcohol. Background technique [0002] Defatted seabuckthorn seed residue is a by-product of seabuckthorn oil extraction, and its protein content is about 30%. At present, the development and utilization of seabuckthorn seed dregs mainly focus on the extraction of proanthocyanidins and flavonoids, while there are few studies on seabuckthorn seed proteins and peptides, and no research on the preparation of hangover active peptides by enzymatic hydrolysis of seabuckthorn seeds. [0003] Medical research shows that long-term excessive drinking or one-time heavy drinking will reduce the activity of alcohol dehydrogenase (ADH), reduce the body's metabolic function of ethanol, and then aggravate the harmful effects of ethanol on the brain, liver and other organs. In recent years, with the enhan...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/34
Inventor 王常青陈彤
Owner 一阳生集团有限公司
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