63results about How to "Strong activation" patented technology

The present invention provides a polypeptide construct comprising a peptide or polypeptide signaling ligand linked to an antibody or antigen binding portion thereof which binds to a cell surface-associated antigen, wherein the ligand comprises at least one amino acid substitution or deletion which reduces its potency on cells lacking expression of said antigen.

The invention relates to a preparation method of high specific surface area graphene with well-developed mesopores. The method comprises the steps of mixing graphene oxide and an activating agent under a protective atmosphere; passing through an activating gas to active the mixture; and finally obtaining the high specific surface area graphene with the well-developed mesopores through processes of washing, filtering and drying. The activating agent is selected any one from potassium hydroxide, sodium hydroxide, phosphoric acid and zinc chloride or a combination of at least two of the above materials. The method is simple in equipment and low in cost, is safe and reliable, has small pollutions and high production efficiency, and is suitable for large-scale production. The obtained graphene has high specific surface area, and is more suitable for migration of electrolyte ions.

The invention discloses pullulanase XWPu2 and the gene thereof. The amino acid sequence of the pullulanase XWPu2 is shown in SEQID No. 1, and the nucleotide sequence of the pullulanase XWPu2 is shown in SEQID No. 2; and the recombinant vector and the recombinant strain of the pullulanase XWPu2 are provided. The pullulanase XWPu2 taking pullulanase as a substrate has the following properties that the optimum temperature is 50 DEG C; the optimum pH is 5.8-6.8; beta-mercaptoethanol having a final concentration of 1 mM, and metal ions Ca<2+>, Mn<2+>, Fe<2+> and K<+1> having a final concentration of 1 mM have a strong activation effect on enzyme; the residual enzyme activities after heat preservation for 140 minutes at 37 DEG C and at 50 DEG C are greater than 84% and greater than 52% in sequence; the residual enzyme activities after treatment for 90 minutes in a buffering solution having a pH of 5.0 and in a buffering solution having a pH of 8.6 are greater than 74% and greater than 57% in sequence; and the specific activity is 383.5 U/mg. Additionally, a qualitative analysis adopting thin-layer chromatography indicates that the pullulanase XWPu2 can be used for thoroughly hydrolyzing pullulanase to generate single maltotriose, hydrolyzing corn amylopectin to generate dextrin, and hydrolyzing cyclodextrin to generate glucose. The researches indicate that the pullulanase XWPu2 has a potential application prospect in the industries such as food, chemical industry, pharmacy and energy.

The invention relates to a volatile organic compound catalytic oxidation catalyst with a high chlorine poisoning resisting efficiency, and a preparation method thereof. The catalyst adopts RuO2 as anactive component, and adopts a SnyTi1-yO2 or MOx-SnyTi1-yO2 mixed metal oxide as a carrier. TiO2 is doped with Sn to realize the regulation of the crystal form of the carrier oxide, so the dispersiondegree of RuO2 on the surface of the carrier is greatly improved while constructing the tin-titanium-based catalyst carrier with a high activation performance; and the catalyst has the characteristicsof high catalytic activity to chlorine-containing volatile organic compounds, strong chlorine poisoning resistance, and high selectivity of the product to CO2, has a good removal effect on common volatile organic compounds, and can be widely used in treatment of chlorine-containing organic waste gases in the fields of pharmaceutical, organic synthesis and paint spraying.

The invention discloses application of 4-hydroxyl-2-pyridone alkaloid in preparing an antitumor product. The 4-hydroxyl-2-pyridone alkaloid is Sambutoxin, and a chemical structure of the 4-hydroxyl-2-pyridone alkaloid is shown in the description. Through researches of animal experiments, cell experiments and the like, the compound is shown to have very strong antitumor activity on varieties of tumor cells including lung cancer, cervical cancer, colon cancer and breast cancer; the compound has the advantages of obvious effects, safety, low toxin, convenience in use and rich and cheap raw material sources; a preparation technology has the advantages of simpleness, high yield, stable characters, convenience in quality control, lower production cost and convenience in application production; anovel medicine source and a safe raw material are provided for treating and researching antitumor and direct related diseases of the antitumor.

The invention discloses facial beautifying apparatus. The facial beautifying apparatus comprises a shell, a steam nozzle, a massage head, a steam switch, a charging port and a battery. The facial beautifying apparatus integrates face steaming equipment and massaging equipment, wherein the massaging equipment adopts radio frequency (RF) wave and ceramic-magnetic-alloy polycrystalline composite wafers prepared by a nano-ultrafine technology. Acting on facial acupoints, the facial beautifying apparatus is capable of effectively penetrating deep skin at a depth up to 9 mm by strong penetration, activation and metabolism performance of bio-ion lightwave of the composite wafers, thereby causing high-frequency vibration at the acupoints so as to activate basal cells and cells in deep sleep; and moreover, water molecules are rapidly atomized by adopting high-frequency atomizing-vibrating, so that absorption is facilitated. In addition, face massage can be performed while carrying out of facialsteaming-beautifying, so that time and labor can be saved; and moreover, the facial beautifying apparatus has reduced space occupation size, and is convenient to carry.

The invention discloses a method for mitigating MBR membrane fouling by using a lanthanide compound, and belongs to the technical field of membrane bioreactors and water treatment. In the sludge culture process of a membrane bioreactor, the lanthanide compound is added into the bioreactor and runs stably after being stirred, the coagulation effect is generated by utilizing the electric neutralization and net capturing sweeping functions of lanthanide, the speed of forming floccules is high, the floccules are compact, the water content is low, the turbidity removal rate is high, the effluent turbidity is low, and membrane fouling in the membrane bioreactor is effectively mitigated. During running, lanthanide mostly exists in activated sludge in the reactor, the problems of easy decomposition of a traditional additive and secondary effluent fouling are solved, and the feasibility of the lanthanide compound serving as the MBR additive in practical application is improved.

The invention relates to a modified manganese oxide catalyst, a preparation method and application thereof. The catalyst is obtained by loading a transition metal element to manganese oxide serving asa carrier, is rich in surface oxygen vacancies on the surface, and has excellent activation capability on oxygen and nitric oxide, so that the catalyst provided by the invention has excellent catalytic activity. Also, the catalyst has excellent selectivity on CO2 during catalytic oxidation of soot particles, and the selectivity on CO2 is close to 100% in a catalytic reaction interval of 300-550DEG C.

A planting method capable of shaping organic carrot ginseng is characterized in that four matched schemes including design of a shaping mould, preparation of nutrient organic fertilizer, preparation of nutrient fluid, and organic planting method, are adopted to perform the whole planting process of shaping carrot ginseng. Therefore, the produced organic carrot ginseng products have a sweet taste, are shaped like ginseng, are uniform in size, and contain high carroty; and the functional substances like quercetin and nucleogelase in the organic carrot ginseng are more than 4 times of those in ordinary carrots, and the overall nutrient value is remarkably improved.

The invention belongs to the field of covalent organic framework materials, in particular to a two-dimensional covalent organic framework material linked by imine bonds and a preparation method and use of the material. The structural unit of the material belongs to the hexagonal crystal system PM space group, the cell parameters are as follows: a is 30.7 A, b is 3.5 A, c is 30.7 A, alpha is 90 degrees, beta is 90 degrees, and gamma is 120 degrees. The material has regular hexagonal and triangular staggered hole-channels, and the aperture sizes are respectively 21 A and 9 A.

The invention discloses a fusion protein based on CjCas9 and VPR core structure domains, a corresponding DNA targeting activation system and application of the system. The fusion protein comprises twoheterologous polypeptide structure domains, and one polypeptide structure domain comprises VPR protein with the transcription activation activity; the other polypeptide structure domain comprises theCjCas9 protein, the CjCas9 protein is in a dCjCas9 subtype or a mini-dCjCas9 subtype or a CjCas9 wild type, and the dCjCas9 subtype contains D8A and H559A single-locus amino acid mutation; the mini-dCjCas9 subtype contains D8A single-locus amino acid mutation and deficiency of most HNH structure domains. The DNA targeting activation system comprises the fusion protein and one or more kinds of guide RNA, and the guide RNA comprises a sequence with the length of 14-22 bp and a framework sequence with the length of 80 bp, wherein the sequences are designed for a target gene promoter area. The DNA targeting activation system is applied to targeting activation of a gene. The DNA targeting activation system has the advantages of being small in size, capable of achieving high specificity, a highactivation effect and easy synthesis, low in cost and the like.

The invention relates to application of engelhardia roxburghiana extract in preparing a medicament for treating cholestatic liver disease. The engelhardia roxburghiana extract ER and ER-A2 have certain FXR activating action, and the engelhardia roxburghiana extract ER-A3 and ER-B2 have relatively strong activating action on FXR and can be directly used as a farnesol X receptor agonist. In addition, the engelhardia roxburghiana extract ER-A3 and ER-B1 prepared by the invention have remarkable inhibiting action on activity rise of Tbil, ALT, APL and gamma-GT caused by cholestasis induced by ANIT, obviously improve the hepatocyte damage, inflammatory cell infiltration and bile excretion caused by ANIT and have relatively good therapeutic action on cholestatic liver disease, and can be used as a potential drug for treating cholestatic liver disease.

The invention relates to a polypeptide, a fusion protein of the polypeptide and helicobacter pylori ferritin and a subunit vaccine prepared from the fusion protein for preventing novel coronavirus (SARS-CoV-2) infection. Specifically, the polypeptide comprises an S1 protein or a fragment thereof (SS0, SS1, SS2 or SS3 protein), and the amino acid sequences of the S1 protein or the fragment thereofare derived from the sequence of an S protein of SARS-CoV-2, and optimization is carried out. The polypeptide can be expressed as a fusion protein by fusion with the helicobacter pylori ferritin performed with codon optimization via a hinge. The fusion protein of the polypeptide and the helicobacter pylori ferritin has the advantages of being high in expression level in CHO cells and easy to purify, and a high-titer neutralizing antibody against the SARS-CoV-2 can be generated after animals are immunized.

The invention discloses a hericium erinaceus polysaccharide chelated zinc microcapsule and a preparation method thereof. The method includes utilizing a hericium erinaceus polysaccharide as a raw material, chelating the hericium erinaceus polysaccharide with zinc ions to form a polysaccharide zinc chelate, performing homogenization emulsification and spray drying with carrageenan: chitosan: beta-cyclodextrin as wall materials and performing microencapsulation treatment to obtain the hericium erinaceus polysaccharide chelated zinc microcapsule. The microcapsule overcomes shortcomings of poor antioxidant activity of the traditional hericium erinaceus polysaccharide and low zinc content in the hericium erinaceus polysaccharide chelated zinc (Zn-HEP), can not only improve the dispersibility and the emulsifying dispersibility, but also protect the easily oxidized components in the polysaccharide, enhance stability and antioxidant activity and prolong the shelf life. The prepared hericium erinaceus polysaccharide chelated zinc microcapsule has obvious anti-oxidation activity, and enhances immune function and anti-cancer effect. The preparation method is simple and convenient, capable ofachieving industrial continuous production, and has wide practical value.

The invention relates to a method for removing organic pollutants by activating persulfate with a diatomite composite material loaded with iron and manganese, which comprises the following steps: (S1) modifying pretreated diatomite with a sulfydryl silane coupling agent, impregnating the modified diatomite in a mixed solution of ferrite and manganese salt, adding an alkaline solution, stirring to obtain slurry, drying the slurry, washing, and drying again to obtain a diatomite composite material loaded with iron and manganese; grinding to obtain a diatomite composite material loaded with iron and manganese; and (S2) adding a persulfate aqueous solution and the composite material obtained in the step (S1) into a water body or soil to be repaired, and fully mixing, so that organic pollutants in an object to be repaired are degraded and removed. The composite material prepared by the invention can efficiently activate persulfate and quickly degrade refractory organic matters in a to-be-repaired system. The method disclosed by the invention is green, safe, environment-friendly and low in cost, does not need to additionally implement measures such as heating and light irradiation, and saves energy and related equipment.

A method for preparing synthesis gas by methane chemical-looping partial oxidation is characterized in that a fuel reactor and a regeneration reactor which are communicated are adopted, an oxygen carrier is circulated between the fuel reactor and the regeneration reactor, the oxygen carrier oxidizes methane in the fuel reactor at 800-950 DEG C, then the oxygen carrier is oxidized and regenerated in the regeneration reactor at 800-950 DEG C, and the oxygen carrier is an Sn-doped perovskite oxygen carrier. According to the method, the oxygen carrier is adopted to effectively inhibit methane fromcracking to generate carbon deposition, after 10 times of circulation, the CO selectivity is still about 80% or above, reaction byproducts are mainly CO2 and H2O, and no carbon deposition is generated. The oxygen carrier has outstanding oxygen carrying capacity, synthesis gas selectivity, carbon deposition resistance, reaction activity and cycling stability.

The invention discloses a preparation technology and application of nanometer-fertilizer-added activation ionized water as nuisance-free crop cultivation nutrient solution. The nutrient solution is composed of highly-basic ionized water and a nanometer metal colloid subjected to enveloping modification, and possesses dual efficacies of a plant growth promoter and a microelement fertilizer, and also possesses functions of sterilization, disinfection and controlling on plant disease insects. Trace elements essential for crops are used to prepare a nanometer structural material and then generate the nanometer metal colloid, and the nanometer metal colloid is added into microcluster alkaline ionized water, so that fertilizing validity of multiple trace elements essential for plants is improved, and the application scope is expanded. The nutrient solution is applicable to ecological agriculture, garden cultivation, greenhouse vegetable plantation, soilless culture and other fields.

The invention provides a bispecific antibody and application thereof, the bispecific antibody contains an antibody or an antibody fragment, and the antibody or the antibody fragment is the bispecific antibody or the antibody fragment with a first heavy chain and a first light chain as well as a second heavy chain and a second light chain; the first heavy chain and the first light chain form a first Fab region; the second heavy chain and the second light chain form a second Fab region; a plurality of mutations are introduced into the first Fab region and the second Fab region. According to the present invention, amino acid size and charge mutation is performed on the interface amino acids of the heavy chain CH1 and the light chain CL of the antibody or the antibody fragment, such that the heavy/light chain correct pairing aiming at different antigens is enhanced, the binding capacity of the specific antigen is maintained, the mismatching is reduced, the binding specificity is improved, and the yield of the target product is improved.