Biosensor and method for producing the same

Inactive Publication Date: 2000-12-19
PANASONIC HEALTHCARE HLDG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

It is therefore a primary object of the present invention to provide a biosensor that can be applied to quantificat

Problems solved by technology

By the above-mentioned method, the measurement is however adversely influenced with a concentration of the dissolved oxygen depending on the subject of the measurement.
Further, the measurement is made completely impossible under a condition lacking oxygen.
the configuration of such prior art sensors, however, there is an inconvenience that an adverse influence is given to the responsive current, because wetting of the surface of the insulating base including the electrode system with the sample solution is not necessarily uniform and thus bubbles are ret

Method used

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  • Biosensor and method for producing the same
  • Biosensor and method for producing the same

Examples

Experimental program
Comparison scheme
Effect test

example 1

(Fructose Sensor I)

FIG. 1 is a cross-sectional side view showing a fructose sensor prepared in accordance with an embodiment of the present invention with its cover and a spacer omitted, and FIG. 2 is an exploded perspective view of the fructose sensor with its reaction layer omitted.

An insulating base i is made of polyethylene terephthalate. On the insulating base 1, there are provided lead wires 2 and 3 of silver by means of screen printing. An electrode system including a working electrode 4 and a counter electrode 5 is also formed on the insulating base 1 by printing an electrically-conductive carbon paste containing a resin binder. Further, an insulating layer 6 is formed on the insulating base 1 by printing an insulating paste. The insulating layer 6 maintains areas of the exposed regions of the working electrode 4 and the counter electrode 5 constant, and partly covers the lead wires 2 and 3.

After the electrode region was prepared in this manner, a mixed aqueous solution comp...

example 2

(Fructose Sensor II)

In a manner similar to that in Example 1, an electrode system composed of the working electrode 4 and the counter electrode 5 was formed on the insulating base 1 made of polyethylene terephthalate by means of screen printing, as shown by FIG. 3. By dropping an aqueous solution (0.5 wt %) of CMC on the electrode system and then drying, a CMC layer was formed. Next, an aqueous solution of the enzyme FDH and the electron acceptor potassium ferricyanide was spread over the CMC layer and then dried to form an FDH-potassium ferricyanide-CMC layer 7. In this case however, the CMC, the FDH as well as the potassium ferricyanide were partially mixed together and formed in a thin film of a thickness of several microns. That is, when the above-mentioned aqueous solution was dropped on the CMC layer, the previously formed CMC layer was once dissolved and then formed a layer 7 in a state partly mixed with the enzyme and the like during the subsequent drying process.

In this cas...

example 3

(Lactic Acid Sensor)

In a manner similar to that in Example 1, an electrode system was formed on the insulating base 1 made of polyethylene terephthalate by means of screen printing, as shown by FIG. 4. By dropping an aqueous solution (0.5 wt %) of CMC, which also dissolved the buffer, potassium dihydrogenphosphate and dipotassium hydrogenphosphate, on the electrode system and then drying, a buffer-CMC layer 11 was formed. Next, an ethanol solution (0.5 wt %) of PVP was spread over the buffer-CMC layer 11 so that it covered the layer, and then dried to form a PVP layer. An aqueous solution of lactic acid oxidase (available from TOYOBO Co., Ltd., hereinafter referred to LOD) as an enzyme and potassium ferricyanide as an electron acceptor was spread over the PVP layer and then dried. In this case, however, since the PVP layer was partly dissolved in the above-mentioned aqueous solution, an LOD-potassium ferricyanide-PVP layer 12 was formed. Further, since the ethanol employed for formi...

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Abstract

A biosensor for rapid quantification of a specific component contained in various biological samples with high accuracy has an electrically insulating base, an electrode system including a working electrode and a counter electrode formed on one face of the insulating base, and a reaction layer formed on the insulating base in close contact with the electrode system. The reaction layer contains at least a hydrophilic polymer, a buffer and an enzyme which is separated from the buffer.

Description

BACKGROUND OF THE INVENTION1. Field of the InventionThe present invention relates to a biosensor capable of rapidly quantifying a specific component in a sample solution with high accuracy in a simplified manner, and to a method for producing the same.2. Description of the Related ArtVarious types of biosensor have heretofore been proposed as a system for quantifying the specific component in the sample solution without requiring diluting or stirring of the sample solution.As an example of such biosensors, a glucose Sensor will be described in the following paragraphs. In general, a system combining glucose oxidase with an enzyme electrode or a hydrogen peroxide electrode is already known as a method of quantifying glucose utilizing the enzyme electrode. The glucose oxidase selectively oxidizes a substrate, i.e., .beta.-D-glucose into D-glucono-.delta.-lactone by using oxygen as an electron acceptor. During this reaction, oxygen is reduced into hydrogen peroxide. By measuring the am...

Claims

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Application Information

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IPC IPC(8): C12Q1/00
CPCC12Q1/002C12Q1/001Y10S435/817
Inventor YAMAMOTO, TOMOHIROMIYASHITA, MARIKOYOSHIOKA, TOSHIHIKOTSUJINANKAI, SHIRO
Owner PANASONIC HEALTHCARE HLDG CO LTD
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