In-vivo collecting device used for liquid biopsy of blood

Abstract

The invention provides a catheter which comprises a catheter body and a collecting device. The front end of the catheter body is connected with the collecting device. The invention provides an in-vivo sample (including circulating tumor cells, exosomes and nucleic acids) collecting device used for liquid biopsy of blood, the in-vivo collecting device can be used for in-vivo collection of the cells, the exosomes and the nucleic acids, and is particularly used to collect the circulating tumor cells; and when being used to collect the tumor cells, the in-vivo collecting device captures and enriches as many tumor cells as possible as compared with the technique of collecting peripheral blood and separating and capturing the tumor cells in the prior art; the in-vivo collecting device is convenient and safe, is easy to operate, and is low in cost; the captured tumor cells are used for downstream analysis, for example gene analysis, in vitro culturing and drug sensitive test; and more importantly, the in-vivo collecting device can block metastasis of the tumor cells through blood after being improved.

Description

technical field [0001] The invention relates to a medical device, in particular to an in vivo collection device for blood liquid biopsy. Background technique [0002] The method commonly used in the prior art to collect tumor cells is: collect 7.5ml of peripheral blood from the patient and centrifuge, use ferrofluid to capture tumor cells, and then use a series of monoclonal antibody immunohistochemistry and nucleic acid dyes to discover tumor cells. However, the disadvantages of this method are: 1. Tumor cells in the circulating blood of patients with solid tumors are extremely rare (about 1 tumor cell / 10 6 -10 7 white blood cells), it is difficult to capture, discover and identify; 2. There are many operation steps, and the operation is cumbersome; 3. The cost of the experiment is high, and the experimental equipment is extremely expensive; 4. The tumor cells identified by separation and capture are difficult to use for downstream analysis, such as gene analysis and in vi...

AI Technical Summary

Problems solved by technology

However, the disadvantages of this method are: 1. Tumor cells in the circulating blood of patients with solid tumors are extremely rare (about 1 tumor cell / 10 6 -10 7 white blood cells), it is difficult to capture, discover and identify; 2. There are many operation steps, and the operation is cumbersome; 3. The cost of the experiment is high, and the experimental equipment is extremely expensive; 4. The tumor cells identified by separation and capture are difficult to use for downstream analysis, such as gene analysis and in vitro culture , drug sensitivity test, etc.

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