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Method for domesticating rhizopus oryzae and method for producing lactic acid

A technology of rhizopus oryzae and lactic acid root, applied in the direction of using microorganisms, methods based on microorganisms, biochemical equipment and methods, etc., can solve the problems of blindness and low stability of strains, and achieve high-efficiency utilization and strain growth Fast speed, weakening effect of product inhibition effect

Active Publication Date: 2021-03-16
TAIZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to overcome the problems of blindness and low stability of the strains selected for breeding in the breeding process of Rhizopus oryzae strains in the prior art, A method for domesticating Rhizopus oryzae and a method for producing lactic acid are provided, the domestication method can obtain a strain with high lactic acid production, high stability and good fermentation performance

Method used

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  • Method for domesticating rhizopus oryzae and method for producing lactic acid
  • Method for domesticating rhizopus oryzae and method for producing lactic acid
  • Method for domesticating rhizopus oryzae and method for producing lactic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Liquid acclimation medium: basal medium + sodium lactate (lactate ion concentrations are 0.1mol / L, 0.2mol / L, 0.4mol / L, 0.5mol / L, 0.6mol / L, 0.8mol / L, 0.9mol / L L, 1mol / L)

[0055] Solid acclimation medium: PDA medium + copper sulfate (copper ion concentrations are 30 μmol / L, 40 μmol / L, 50 μmol / L, 60 μmol / L, 70 μmol / L, 80 μmol / L, 90 μmol / L, 100 μmol / L)

[0056] Fermentation medium: basal medium + copper sulfate (copper ion concentration is 60 μmol / L)

[0057] (1) Inoculate the original bacterial strain of Rhizopus oryzae on 50mL basal medium, the inoculum size of Rhizopus oryzae is 5 * 10 4 cfu / mL, under the condition that the temperature is 25°C and the rotation speed is 200rpm, the activated culture is carried out until the dry cell weight is 3g / L (counted as one generation), and the activated Rhizopus oryzae liquid is obtained;

[0058] (2) the rhizopus oryzae activated in step (1) is inoculated in the solid-state domestication medium with a copper ion concentration o...

Embodiment 2

[0067] Liquid acclimation medium: basal medium + mixture of sodium lactate and calcium lactate (the molar ratio of the two is 3:1) (lactate ion concentrations are 0.1mol / L, 0.25mol / L, 0.4mol / L, 0.55mol / L , 0.7mol / L, 0.85mol / L)

[0068] Solid acclimation medium: PDA medium + copper chloride (copper ion concentrations are 30 μmol / L, 45 μmol / L, 60 μmol / L, 75 μmol / L, 90 μmol / L, 100 μmol / L)

[0069] Fermentation medium: basal medium + copper chloride (copper ion concentration is 80 μmol / L)

[0070] (1) Inoculate the original bacterial strain of Rhizopus oryzae on 50mL basal medium, the inoculum size of Rhizopus oryzae is 6 * 10 4 cfu / mL, under the condition that the temperature is 20°C and the rotation speed is 250rpm, the activated culture is carried out until the dry cell weight is 5g / L (counted as one generation), and the activated Rhizopus oryzae liquid is obtained;

[0071] (2) the rhizopus oryzae activated in step (1) is inoculated in the solid-state domestication medium wi...

Embodiment 3

[0080] Liquid acclimation medium: basal medium + mixture of sodium lactate, calcium lactate, and magnesium lactate (the molar ratio of the three is 4:3:3) (the concentration of lactate ion is 0.1mol / L, 0.28mol / L, 0.46mol / L respectively , 0.64mol / L, 0.82mol / L, 1mol / L)

[0081] Solid acclimation medium: PDA medium + copper sulfate (copper ion concentrations are 30 μmol / L, 42 μmol / L, 54 μmol / L, 66 μmol / L, 78 μmol / L, 90 μmol / L)

[0082] Fermentation medium: basal medium + copper chloride (copper ion concentration is 90 μmol / L)

[0083] (1) Inoculate the original bacterial strain of Rhizopus oryzae on 50mL basal medium, the inoculum size of Rhizopus oryzae is 8 * 10 4 cfu / mL, under the condition that the temperature is 20°C and the rotation speed is 150rpm, the activated culture is carried out until the dry cell weight is 4g / L (counted as one generation), and the activated Rhizopus oryzae liquid is obtained;

[0084] (2) the rhizopus oryzae activated in step (1) is inoculated in ...

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Abstract

The invention relates to microbial fermentation, and discloses a method for domesticating rhizopus oryzae and a method for producing lactic acid. The method for domesticating rhizopus oryzae comprisesthe following step: domesticating rhizopus oryzae by utilizing a domestication culture medium containing lactate ions and / or copper ions. The invention also provides application of the domesticated rhizopus oryzae strain obtained by the method for domesticating rhizopus oryzae in production of lactic acid. The invention further provides a method for producing lactic acid, which comprises the following step: fermenting the domesticated rhizopus oryzae strain obtained by domestication according to the method. Directional domestication transformation of rhizopus oryzae with high lactic acid yield is realized, the method has very strong objectivity, the domestication method is simple and convenient, the efficiency is high, and the obtained domesticated rhizopus oryzae strain is strong in hereditary stability, high in strain growth speed and high in lactic acid yield.

Description

technical field [0001] The invention relates to microbial fermentation, in particular to a method for acclimating Rhizopus oryzae and a method for producing lactic acid. Background technique [0002] Lactic acid is a common carboxylic acid that can be used as a bacteriostatic agent, flavor enhancer, pH regulator, etc. In addition, the polylactic acid material synthesized from lactic acid is biodegradable and can be used in the production of medical sutures , food packaging materials, etc. Biodegradable polylactic acid materials have the potential to replace petroleum-based plastic products, and are currently a hot spot in material research. At present, more than 90% of lactic acid is produced by microbial fermentation, and Rhizopus oryzae is a common strain for biosynthesizing lactic acid. [0003] At present, the techniques for fermenting and producing lactic acid by Rhizopus oryzae mostly focus on the three aspects of strain selection, control of cell morphology, and con...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/36C12N1/38C12P7/56C12R1/845
CPCC12N1/36C12N1/38C12P7/56
Inventor 尹丰伟孙小龙付永前郑伟龙罗希张莹莹尹龙飞贾强孔钰婷
Owner TAIZHOU UNIV
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