Method for extracting L-isoleucine

A technology of isoleucine and strong acid cation, applied in the field of extracting L-isoleucine to achieve the effects of reducing dosage, protecting natural environment, and improving extraction rate and quality

Active Publication Date: 2011-05-04
梅花生物科技集团股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of this, the present invention provides a method for extracting L-isoleucine, which effectively solves the problem of the increase of heteroamino acids in the eluate due to the one-time collection of the eluate in the ion exchange method, which affects the production of L-isoleucine. Improve the extraction rate and quality of L-isoleucine

Method used

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  • Method for extracting L-isoleucine
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Effect test

Embodiment 1

[0036] Embodiment 1: The method of the present invention extracts L-isoleucine

[0037] 1. Extraction method

[0038] For the enzyme-inactivated L-isoleucine fermentation broth, adjust the pH of the fermentation broth to 3 with hydrochloric acid, add chitosan in an amount of 0.1%, maintain the flocculation temperature at 20° C., and stir for 30 minutes. The pore size is 50nm, the molecular weight is 30KD, and the area is 117m 2 The ceramic membrane is used for microfiltration of the clarified solution after flocculation, the operating pressure of the ceramic membrane is controlled to be 0.2Mpa, the temperature of the membrane is 40°C, the amount of dialyzed water added is 30% (volume ratio) of the fermentation broth, and the filtrate is mixed with L-isoleucine The concentration ratio of the fermentation broth is 1:6. The filtrate is directly adsorbed on the 732-type strong acid cationic resin, and the column loading speed is 0.2m / s. After the adsorption is saturated, the re...

Embodiment 2

[0044] Embodiment 2: The method of the present invention extracts L-isoleucine

[0045] 1. Extraction method

[0046] For the enzyme-inactivated L-isoleucine fermentation broth, adjust the pH of the fermentation broth to 3 with hydrochloric acid, add aluminum chloride in an amount of 4%, maintain the flocculation temperature at 70°C, and stir for 120 minutes. The pore size is 50nm, the molecular weight is 30KD, and the area is 117m 2 The ceramic membrane is used for microfiltration of the clarified solution after flocculation, the operating pressure of the ceramic membrane is controlled to be 0.8Mpa, the temperature of the membrane is 60°C, the amount of dialyzed water added is 50% (volume ratio) of the fermentation broth, and the filtrate is mixed with L-isoleucine The concentration ratio of the fermentation broth is 1:10. The filtrate is directly adsorbed on the JK008 type strong acid cationic resin, and the column loading speed is 1m / s. After the adsorption is saturated,...

Embodiment 3

[0052] Embodiment 3: The method of the present invention extracts L-isoleucine

[0053] 1. Extraction method

[0054] For the enzyme-inactivated L-isoleucine fermentation broth, adjust the pH of the fermentation broth to 3 with hydrochloric acid, add aluminum chloride in an amount of 4%, maintain the flocculation temperature at 45°C, and stir for 60 minutes. The pore size is 50nm, the molecular weight is 30KD, and the area is 117m 2 The ceramic membrane is used for microfiltration of the clarified solution after flocculation, the operating pressure of the ceramic membrane is controlled to be 0.5Mpa, the temperature of the membrane is 50°C, the amount of dialyzed water added is 40% (volume ratio) of the fermentation broth, and the filtrate is mixed with L-isoleucine The concentration ratio of the fermentation broth is 1:8. The filtrate is directly adsorbed on the JK006 type strong acid cationic resin, and the column loading speed is 0.6m / s. After the adsorption is saturated,...

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Abstract

The invention discloses a method for extracting L-isoleucine, belonging to the field of biochemical engineering. The method disclosed by the invention comprises the following steps of: flocculating an L-isoleucine fermentation liquid, and micro-filtering the flocculated clear solution by using a ceramic membrane with pore diameter of 50nm and molecular weight of 30KD; adsorbing the filtration liquid obtained by micro-filtration by using strong acid cation exchange resin, eluting the strong acid cation exchange resin by using 0.2-0.6mol / L ammonia water or sodium hydroxide, collecting an elution liquid A from the starting of elution to the moment when the pH value of the elution liquid is 7-8, and collecting an elution liquid B from the moment when the pH value of the elution liquid is 7-8 to the moment when the pH value of the elution liquid is 9.5-10.5; concentrating, filtering and decolorizing the elution liquid A, crystallizing to obtain L-isoleucine crystals, and repeating ion exchange adsorption on the elution liquid B. The method can be used for effectively removing impurities in the filtration liquid and the elution liquids, increases the extraction rate and the quality of L-isoleucine and can be widely applied to the production of the L-isoleucine.

Description

technical field [0001] The invention relates to the field of biochemical industry, and specifically provides a method for extracting L-isoleucine. Background technique [0002] Isoleucine, scientific name 2-amino-3-methylvaleric acid, alias isoleucine, is one of the 20 basic amino acids, which exists in almost all protein structures. Isoleucine has 2 asymmetric carbon atoms, so there are 4 stereoisomers and 2 diastereomers of L-isoleucine, but only one type of L-isoleucine exists in nature . Since L-isoleucine cannot be synthesized in the human body, it is an essential amino acid for the human body. It is collectively referred to as branched-chain amino acids together with L-leucine and L-valine. plays an important role in. [0003] Internationally, there are four major L-isoleucine manufacturers including Ajinomoto, Kyowa Hakko, Tanabe Pharmaceutical, and Degussa, Germany, all of which use fermentation to produce L-isoleucine. Among them, Japan is in the leading positio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C229/08C07C227/40
Inventor 王海雷钟秀茹龚华常利斌
Owner 梅花生物科技集团股份有限公司
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