Non-serum non-animal-origin-additive insect cell culture medium
A technology of insect cells and animal sources, applied in the direction of invertebrate cells, animal cells, cell culture active agents, etc., can solve the problems of high requirements for transportation and bacteria release, high price, unsuitable for the development of medium, etc., to achieve Short doubling time, low cost, and easy preparation
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Embodiment 1
[0021] Embodiment 1 A kind of serum-free insect cell culture medium without animal source added
[0022] A serum-free insect culture medium without animal-derived additives, consisting of the following components and their concentrations: Grace medium 7.5g / L, glucose 7.2g / L, sodium chloride 21.5mM, sodium dihydrogen phosphate 11mM, citric acid Sodium 5.5mM, sodium selenite 13.5mM, potassium chloride 6mM, potassium sulfate 5.23mM, aluminum chloride 2.75mM, zinc sulfate 14.5mM, manganese chloride tetrahydrate 3.2mM, ammonium molybdate 0.67mM, biotin 1.75mg / L, vitamin B 12 0.38mg / L, riboflavin 1.82mg / L, p-aminobenzoic acid 0.35mg / L, pantothenic acid 7.6mg / L, vitamin B 1 0.71mg / L, folic acid 0.27mg / L, choline chloride 17mg / L, L-arginine 5.6mM, L-asparagine 4.75mM, L-glycine 8.2mM, L-histidine 2.45mM, L -Isoleucine 8.65mM, L-Leucine 7.68mM, L-Lysine 8.15mM, L-Methionine 0.49mM, L-Threonine 7.2mM, L-Tryptophan 1.14mM, L-Kytidine Acid 3.32mM, L-proline 8.12mM, L-glutamine 13.75g / ...
Embodiment 2
[0028] Embodiment 2 A kind of serum-free insect cell culture medium without animal source added
[0029] A serum-free insect culture medium without animal-derived additives, consisting of the following components and their concentrations: basal medium 6g / L, glucose 6.5g / L, sodium chloride 20mM, sodium dihydrogen phosphate 10mM, sodium citrate 5mM, sodium selenite 12mM, potassium chloride 5mM, potassium sulfate 4mM, aluminum chloride 1mM, zinc sulfate 10mM, manganese chloride tetrahydrate 1mM, ammonium molybdate 0.3mM, malic acid 0.4g / L, fumaric acid 0.1g / L, biotin 0.15mg / L, vitamin B 12 0.3mg / L, riboflavin 1.5mg / L, p-aminobenzoic acid 0.21mg / L, pantothenic acid 7mg / L, vitamin B 1 0.69mg / L, folic acid 0.2mg / L, choline chloride 16mg / L, L-arginine 4mM, L-asparagine 3mM, L-glycine 7mM, L-histidine 1.5mM, L-isoleucine Glycine 8.5mM, L-Leucine 7mM, L-Lysine 4mM, L-Methionine 0.35mM, L-Threonine 4.5mM, L-Tryptophan 0.69mM, L-Gyptophan 2.87mM, L - Proline 7.38mM, L-Glutamine 7g / L,...
Embodiment 3
[0031] Embodiment 3 A kind of serum-free insect cell culture medium without animal source added
[0032] An insect culture medium without serum and animal-derived additives, consisting of the following components and their concentrations: basal medium 8g / L, glucose 8g / L, sodium chloride 23mM, sodium dihydrogen phosphate 12mM, sodium citrate 7mM , sodium selenite 15mM, potassium chloride 8mM, potassium sulfate 6mM, aluminum chloride 3.5mM, zinc sulfate 20mM, manganese chloride tetrahydrate 5mM, ammonium molybdate 0.7mM, malic acid 1.8g / L, fumaric acid 0.8g / L, biotin 2.75mg / L, vitamin B 12 0.42mg / L, riboflavin 2.3mg / L, p-aminobenzoic acid 0.42mg / L, pantothenic acid 8mg / L, vitamin B10.74mg / L, folic acid 0.3mg / L, choline chloride 18mg / L, L- Arginine 6mM, L-Asparagine 5mM, L-Glycine 10mM, L-Histidine 3mM, L-Isoleucine 9mM, L-Leucine 9mM, L-Lysine 9mM, L-Methionine 0.54 mM, L-threonine 7.5mM, L-tryptophan 1.2mM, L-glutine 4.32mM, L-proline 8.42mM, L-glutamine 14.5g / L, yeast extra...
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