Method for extracting L-isoleucine through whole membrane technology
A technology for isoleucine and membrane extraction, which is applied in the field of whole membrane extraction of L-isoleucine, can solve the problem of not reaching the level of pharmaceutical grade, no formation of L-isoleucine, product yield and purity need to be improved and other problems, to achieve the effects of good flocculation and impurity removal, increased corporate income, and improved yield and purity
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[0041] A method for extracting L-isoleucine from a whole membrane, comprising the steps of:
[0042] Step 1) Microfiltration membrane filtration and flocculation to remove impurities: 1350kg of L-isoleucine fermentation broth with a mass content of 1.83% was filtered through a microfiltration membrane to obtain the microfiltration dialysate and bacterial protein of the fermentation broth. The protein is sold as high-quality bacterial protein after drying; then add flocculant accounting for 1% (w / w) of the microfiltration dialysate, stir at 200 rpm for 10 minutes, then let it stand for 120 minutes, and filter it twice with the microfiltration membrane to collect Precipitate and supernatant;
[0043] The conditions for the primary filtration and secondary filtration of the microfiltration membrane are: the microfiltration membrane is an inorganic ceramic membrane, the molecular weight cut-off is 10000Da, and the microfiltration temperature is 50°C;
[0044] Step 2) Ultrafiltrat...
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