Method for extracting L-isoleucine through whole membrane technology

A technology for isoleucine and membrane extraction, which is applied in the field of whole membrane extraction of L-isoleucine, can solve the problem of not reaching the level of pharmaceutical grade, no formation of L-isoleucine, product yield and purity need to be improved and other problems, to achieve the effects of good flocculation and impurity removal, increased corporate income, and improved yield and purity

Active Publication Date: 2016-12-14
SHANDONG FUFENG FERMENTATION CO LTD
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AI Technical Summary

Problems solved by technology

At present, the application of membrane technology at home and abroad mostly uses a single membrane to solve a specific problem, and there is no process for extracting L-isoleucine by the whole membrane method.
Although the applicant's previous patented technology 2009100187695 also uses membrane extraction technology, this technology is not affected by temperature and does not damage heat-sensitive substances, but the product yield and purity still need to be improved, which cannot reach the level of pharmaceutical grade, and urgently needs to be improved

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] A method for extracting L-isoleucine from a whole membrane, comprising the steps of:

[0042] Step 1) Microfiltration membrane filtration and flocculation to remove impurities: 1350kg of L-isoleucine fermentation broth with a mass content of 1.83% was filtered through a microfiltration membrane to obtain the microfiltration dialysate and bacterial protein of the fermentation broth. The protein is sold as high-quality bacterial protein after drying; then add flocculant accounting for 1% (w / w) of the microfiltration dialysate, stir at 200 rpm for 10 minutes, then let it stand for 120 minutes, and filter it twice with the microfiltration membrane to collect Precipitate and supernatant;

[0043] The conditions for the primary filtration and secondary filtration of the microfiltration membrane are: the microfiltration membrane is an inorganic ceramic membrane, the molecular weight cut-off is 10000Da, and the microfiltration temperature is 50°C;

[0044] Step 2) Ultrafiltrat...

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Abstract

The invention belongs to the technical field of base acid production, and discloses a method for extracting L-isoleucine through whole membrane technology. The method comprises the following steps: step 1) filtering through a micro-filtration membrane and removing impurities through flocculation; step 2) filtering through an ultra-filtration membrane, step 3) concentrating through a reverse osmosis membrane; step 4) concentrating and crystallizing. The membrane technology is combined with the flocculation agent to remove the impurities, the process is simple and feasible, and the yield and the purity of the product are greatly improved.

Description

technical field [0001] The invention belongs to the technical field of amino acid production, and in particular relates to a method for extracting L-isoleucine from a whole membrane. Background technique [0002] L-isoleucine (L-isoleucine) is an essential amino acid for the human body and one of the three branched-chain amino acids. Because of its special structure and function, it plays a particularly important role in the metabolism of human life. There are 2 asymmetric carbon atoms in its molecule, so there are 4 stereoisomers, and only L-isoleucine exists in nature. As one of the eight essential amino acids for the human body, L-isoleucine is widely used in the fields of medicine, food, cosmetics and agriculture. In food, it is mainly used to strengthen and balance various amino acids and improve the nutritional value of food. In medicine, the compound amino acid infusion composed of three kinds of branched chain amino acids and a large number of therapeutic special a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C227/40C07C227/42C07C229/08C07K1/34C12N1/00B01D61/14B01D37/03B01D9/02
CPCB01D9/0004B01D37/03B01D61/145B01D61/147C12N1/00C07C227/40C07C227/42C07K1/34B01D61/146C07C229/08
Inventor 贾召鹏赵兰坤冯珍泉刘元涛李树标徐田华刘超
Owner SHANDONG FUFENG FERMENTATION CO LTD
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