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Cleaning production process of extracting L-isoleucine from fermented liquor using ion-exchange

An ion exchange method and isoleucine technology are applied in the field of zero discharge of ammonium-containing wastewater, which can solve the problems of treating symptoms but not the root cause, difficulty in offsetting project construction capital investment and operation costs, and difficulty in rooting out the problems, so as to reduce production costs and reduce environmental protection treatment. Difficulty and the effect of improving economic efficiency

Active Publication Date: 2006-07-12
WUXI JINGHAI AMINO ACID
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But this technology is a negative pollution control method that treats the symptoms but not the root cause, especially for a large amount of high-concentration ammonium-containing wastewater, it is difficult to cure; from a technical point of view, the development of terminal treatment technology is no less difficult than the development of production process technology , and the bigger problem is the economic problem. The small amount of output of the end treatment technology is difficult to offset the capital investment of the project construction and the daily expensive operation costs. The larger the production scale, the more unbearable it is

Method used

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  • Cleaning production process of extracting L-isoleucine from fermented liquor using ion-exchange

Examples

Experimental program
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Effect test

Embodiment 1

[0025] The extraction of embodiment 1, L-isoleucine

[0026] 1) After the fermentation of L-isoleucine is completed, directly heat the fermenter to 80-85° C. for 15-20 minutes, then filter, remove the slag to obtain the filtrate, and store it in the flocculation tank.

[0027] 2) Adjust the pH of the filtrate to 2.0-2.5, add chitosan to a concentration of 30-100mg / L, let stand at 20-50°C for 30-60 minutes, then filter, and store the filtrate in a storage tank.

[0028] 3) Adsorption with strong acidic hydrogen ion exchange resin column, flow rate 0.5 ~ 10m 3 / m 3 resin hours.

[0029] 4) After the adsorption is saturated, rinse with hot water to remove impurities, and then use 0.2-1.0 molar concentration of ammonia water, add ammonium-containing wastewater (ammonia water: ammonium-containing wastewater volume ratio is 1:0.5-1.5) to elute, and the flow rate is 0.5-10m 3 / m 3 resin hours.

[0030] 5) Add the low-concentration eluted components in step 6 to the eluent to adj...

Embodiment 2

[0033] Embodiment 2, the shaking flask fermentation test that replaces ammonium sulfate in the culture medium with ammonium-containing waste water

[0034] Get the ammonium sulfate in the ammonium sulfate in the fermented medium that the ammonium-containing waste water that ion-exchange resin regeneration produces among the embodiment 1 replaces partly, be used for the shake flask test of fermentative production:

[0035] 1) The strain used, Brevibacterium lactofermentum (Brevibacterium lactofermentum) ATCC 13869;

[0036] 2) Fermentation medium formula, glucose 140g / L, corn steep liquor 20g / L, ammonium sulfate 10g / L, ammonium-containing wastewater 100ml / L, biotin 50μg / L, KH 2 PO 4 1g / L, MgSO 4 ·7H 2 O0.5g / L, CaCO 3 15g / L, adjust the pH to 6.8-7.0 with NaOH;

[0037] 3) Liquid volume, 25ml fermentation broth in 50ml triangular bottle;

[0038] 4) Fermentation conditions, the vibration frequency of the reciprocating shaker is 100 times / min, and the fermentation temperatur...

Embodiment 3

[0041] Embodiment 3, the fermentation test of the 5L fermenter that replaces ammonium sulfate in the culture medium with ammonium-containing waste water

[0042] 1) The strains used are the same as in Example 2;

[0043] 2) Seed medium, glucose 25g / L, corn steep liquor 40g / L, ammonium sulfate 5g / L, KH 2 PO 4 10g / L, MgSO 4 ·7H 2 O5g / L, CaCO 3 10g / L;

[0044] 3) Inoculation amount, 5%;

[0045] 4) fermentation medium, with embodiment 2;

[0046] 5) Liquid volume, 3L;

[0047] 6) Fermentation conditions, ventilation rate 180L / hour; stirring speed 500-600 rpm, fermentation temperature 32°C;

[0048] 7) Fermentation cycle, 72 hours;

[0049] 8) Acid production rate, 24.7g / L.

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Abstract

The invention relates to a clean producing craft for extracting L-isoleucine from fermentation liquor in the field of biology chemical technology. It comprises: preparing for the L-isoleucine, regenerating ion exchange column, cycle using the regenerated ammonia sulfate waste liquid; the L-isoleucine preparing course comprises: heat filtering the fermentation liquor, adjusting acid, flocculation filtering, using strong acidic hydrion exchange resin column to do negative pressure to the elutriation liquid, washing it after saturation adsorption to expel the foreign substance, adding ammonia with ammonium waste water to do elutriation, using strong acidic hydrion exchange resin column to do negative pressure to the L-isoleucine again, washing it after saturation adsorption to expel the foreign substance, adding ammonia to do elutriation to obtain the L-isoleucine product. The ion exchange column after elutriating uses sulphuric acid to regenerate; part of the ammonia sulfate waste fluid is used in the bulk of the ferment customs agency; the other part is as the eluent of first ion exchange after adding ammonia, which forms a closed circuit.

Description

technical field [0001] The ion exchange method is a clean production process for extracting L-isoleucine from fermentation broth, specifically related to the separation and purification of amino acid fermentation products and the realization of zero discharge of ammonium-containing wastewater after ion exchange resin regeneration. It belongs to the field of biochemical technology. Background technique [0002] L-isoleucine is one of the eight essential amino acids for the human body and one of the three branched-chain amino acids. Because of its special structure and function, it plays a particularly important role in life metabolism. It is mainly used for general nutritional compound amino acid infusion, and it is widely used in the preparation of special amino acid infusion for treatment, such as Ganan and Shenan amino acid infusion, especially the highly branched chain amino acid infusion, Ganan syrup, Ganling Oral Liquid has significant curative effect on treating vario...

Claims

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Application Information

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IPC IPC(8): C07C227/40C07C229/06C12P13/06
Inventor 蔡立明宁健飞
Owner WUXI JINGHAI AMINO ACID
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