Carbonyl reductase expressed recombination engineering bacterium and application thereof

A technology of recombinant engineering bacteria and reductase, applied in the direction of microorganism-based methods, bacteria, microorganisms, etc., can solve the problems of carbonyl reductase expression level and insufficient enzyme activity, and achieve the effect of optical purity and high conversion rate

Active Publication Date: 2013-04-03
NINGBO MENOVO PHARMA +1
View PDF0 Cites 16 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The invention provides a recombinant engineering bacterium expressing carbonyl reductase to...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Carbonyl reductase expressed recombination engineering bacterium and application thereof
  • Carbonyl reductase expressed recombination engineering bacterium and application thereof
  • Carbonyl reductase expressed recombination engineering bacterium and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0033] (1) strain

[0034] Kluyveromyce marxianus CGMCC2.1977 can be purchased from China General Microorganism Culture Collection Center (CGMCC).

[0035] Escherichia coli DH5α is a commercial product, and Escherichia coli (E.coli) Rosetta (DE3) competent cells were purchased from Biotech Biotech.

[0036] (2) culture medium

[0037] PYD solid medium: glucose 10g, yeast extract 10g, peptone 20g, agar 15g, add water to 1000mL, pH natural.

[0038] PYD liquid medium: glucose 10g, yeast extract 10g, peptone 20g, add water to 1000mL, pH natural.

[0039] LB solid medium: tryptone 10g, yeast extract 5g, NaCl 10g, agar 15g, add water to 1000mL, pH7.0.

[0040] LB liquid medium: tryptone 10g, yeast extract 5g, NaCl 10g, add water to 1000mL, pH7.0.

[0041] 2. Cloning of the target gene

[0042]Pick K. marxianus (K.marxianus) CGMCC2.1977 preserved strain and activate it on agar slant medium at 28°C for 48h, then inoculate it in 30mL PYD liquid medium, put it in a 250mL conical f...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a carbonyl reductase expressed recombination engineering bacterium and application thereof. The engineering bacterium comprises a host cell and a recombinant vector transferred into the host cell, wherein the recombinant vector consists of an original vector and a target gene connected into the original vector, the host cell is Escherichiacoli Rosetta (DE3), the target gene is a carbonyl reductase gene, and the base sequence is shown in SEQ ID NO.1. According to the recombination engineering bacterium, the expression level of a carbonyl reductase is high, bacterial cells which are prepared through induced culture are used for converting N,N-dimethyl-3-oxy-3-(2-thienyl)-l-propylamine hydrochloride into (S)-N,N-dimethyl-3-hydroxy-3-(2-thienyl)-l-propylamine or converting 4-chloroethyl acetoacetate into (S)-4-chloro-3-hydroxyethyl butyrate, and the two products are higher in both optical purity and conversion rate.

Description

technical field [0001] The invention relates to a recombinant engineering bacterial strain, in particular to a recombinant engineering bacterial expressing carbonyl reductase and application thereof. Background technique [0002] Duloxetine (duloxetine), the chemical name is (+)-(S)-N-methyl-γ-yl-(1-naphthyloxy)-2-thienyl hydrochloride, which is the effective drug of Cymbalta Element. It is a dual serotonin (5-HT) and norepinephrine (NE) reuptake inhibitor that treats depression by increasing serotonin and norepinephrine levels in the synaptic cleft, and by blocking Disrupting pain signals to the brain to treat neuropathic pain. On August 3, 2004, the drug was approved by the U.S. Food and Drug Administration for marketing in the United States for the treatment of major depression, and on September 3 of the same year, it was approved for the treatment of diabetic peripheral neuropathic pain (DPN). At present, it has become the most outstanding representative of 3-aryloxy-...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N1/21C12P17/00C12P7/62C12R1/19
Inventor 金志华胡升杨岳微姚成志王爱珂金庆超杨郁梅乐和高然
Owner NINGBO MENOVO PHARMA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap