Composite hydrogel for promoting wound healing as well as preparation method and application thereof

A composite hydrogel, wound healing technology, used in medical science, bandages, etc., can solve the problems of decreased performance and tissue adhesion, unable to support tissue regeneration, poor tissue adhesion, etc., to improve water solubility, increase new Collagen deposition and vascular remodeling, low cost effect

Active Publication Date: 2020-12-25
CHANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Biofibrin glue has been shown to adhere poorly to tissue and fail to support tissue regeneration
Additionally, the mechanical properties and prolonged cure times of existing adhesives often result in reduced performance and tissue adhesion

Method used

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  • Composite hydrogel for promoting wound healing as well as preparation method and application thereof
  • Composite hydrogel for promoting wound healing as well as preparation method and application thereof
  • Composite hydrogel for promoting wound healing as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1 composite hydrogel preparation

[0031] Dissolve 1 g of gelatin in 10 mL of ultrapure water to prepare a 10% solution, heat the solution to 40° C. and stir for 30 minutes. Add 0.2g recombinant human type III collagen, mix and stir for 5 minutes, then add antibacterial photodynamic peptide AMP 2 - Ce6 (final concentration: 0.4 μM, 0.8 μM, 1.6 μM, 2.4 μM, 3.2 μM, 4 μM) was prepared into a gelatin-collagen mixed solution containing antimicrobial peptides. 1ml of 10% transglutaminase aqueous solution was added to 10ml of gelatin-collagen mixed solution to carry out biological enzyme cross-linking for 1 minute. Dispense it into a disposable gel injector mold and let it stand for 30 minutes to form a gel (save at 4°C for later use).

Embodiment 2

[0032] Example 2 Composite Hydrogel Toxicity Test

[0033] The biocompatibility test of mouse fibroblasts (L929) was carried out by MTT method. First, collect the logarithmic phase cells, adjust the concentration of the cell suspension by 10 4 Cells / well were seeded on a 96-well plate. After overnight culture, they were treated with different concentrations of composite hydrogels (0, 0.5, 1, 2, 4, 8, 16 μM), incubated for 24 hours, and then treated with MTT solution. , cell viability was assessed by microplate reader assay (see figure 2 ).

Embodiment 3

[0034] Embodiment 3 wound repair experiment

[0035] The specific experimental steps are:

[0036] (1) Mouse back trauma model

[0037] A total of 35 mice were similar in terms of species, age, and physique, and each mouse had one wound. First, a full-thickness wound with a diameter of about 1 cm was created on the back of Balb / c mice by surgical operation, and then bacteria were inoculated on the wound (10 5 CFU / mL of Staphylococcus aureus) for 2 days, in situ infection to form a biofilm. During the modeling period, the mice were housed in a single cage, accompanied by toys, and allowed to drink water and eat freely. After the modeling, there was no obvious difference in the wound of each mouse, the bacterial infection site was yellow, the periphery was red, and the surrounding tissue edema was obvious.

[0038] (2) Animal grouping

[0039] 35 mice were randomly divided into 7 groups:

[0040] Group A was the control group, and the dosage was 200 μL PBS / wound, applied e...

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PUM

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Abstract

The invention belongs to the field of biological medicine, and particularly relates to composite hydrogel for promoting wound healing as well as a preparation method and application thereof. The composite hydrogel is composed of gelatin, antibacterial photodynamic peptide and recombinant human III-type collagen. A photosensitizer Ce6 is introduced for photodynamic antibacterial chemotherapy antibiosis, and the photosensitizer Ce6 generates active oxygen under laser irradiation to cause microbial molecule oxidation and bacterial cell damage and death, so that the photosensitizer Ce6 and antibacterial peptide generate a synergistic effect. The antibacterial composite hydrogel has high biocompatibility, keeps a wet environment around a wound surface, can stably convey loaded antibacterial photodynamic peptide and recombinant human collagen to the surface of the wound, guides fibroblasts to migrate and proliferate and increases deposition of new collagen on blood vessels for reconstructionin a dermal repair process, thereby accelerating wound healing, thereby improving wound healing quality.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a composite hydrogel capable of promoting wound healing, a preparation method and application thereof. Background technique [0002] As the natural protective barrier of the human body, the skin can protect various tissues and organs in the body from external physical, chemical and pathogenic microorganisms. Following a skin injury, there is often a risk of bacterial infection. During normal wound healing, the stages of hemostasis, inflammation, proliferation, and remodeling all proceed in an orderly and efficient manner. However, persistent infection results from repeated tissue injury or underlying physiologic conditions, such as granuloma or abscess formation following Staphylococcus aureus infection. [0003] Hydrogels hold great promise as wound dressings in the treatment of skin wounds. Hydrogels are natural or synthetic polymers with three-dimensional (3D) network...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L26/00
CPCA61L26/008A61L26/0095A61L26/0066A61L2300/252A61L2300/404A61L2300/224A61L2300/412C08L89/00
Inventor 王建浩雷晓玲李颖芮雯王佳炜邱琳周舒文崔朋飞洪婷婷王程
Owner CHANGZHOU UNIV
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