Engineered electrode for electrobiocatalysis and process to construct the same

a biocatalysis and electrode technology, applied in the field of bioelectrochemical systems, can solve the problems of biofilm stability on the electrode, less attractive appearance, and system like microbial electro-synthesis

Pending Publication Date: 2020-04-30
INDIAN OIL CORPORATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0045]FIG. 1 depicts the graphical representation of electrochemical analysis of bio-electrode constructed by process described in Example 1 and its comparison in four phases, viz immediately after preparation, after 3 months of storage, after 6 months of storage and after 1 year of storage with the control electrode.
[0046]FIG. 2 depicts the graphical representation of electrochemical analysis of bio-electrode constructed by process described in Example 2 and its comparison in four phases, viz immediately after preparation, after 3 months of storage, after 6 months of storage and after 1 year of storage with the control electrode.
[0047]FIG. 3 depicts the graphical representation of electrochemical analysis of bio-electrode constructed by process described in Example 3 and its comparison in four phases, viz immediately after preparation, after 3 months of storage, after 6 months of storage and after 1 year of storage with the control electrode.

Problems solved by technology

This long duration for production of biofilm makes the BES less attractive.
Moreover, a system like microbial electro-synthesis where CO2 is converted to organic chemicals, suffers from stability of biofilm on the electrode.
This delicate biofilm gets disrupted many times and takes a long time to regenerate.
The techniques described in the art require a very long duration (around 3-5 weeks) to make a biofilm on the electrode which is also unstable and subsequently it is immobilized, this as such does not reduce the duration in making effective electro-active biofilm.

Method used

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  • Engineered electrode for electrobiocatalysis and process to construct the same
  • Engineered electrode for electrobiocatalysis and process to construct the same

Examples

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examples

[0110]The disclosure will now be illustrated with working examples, which is intended to illustrate the working of disclosure and not intended to take restrictively to imply any limitations on the scope of the present disclosure. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood to one of ordinary skill in the art to which this disclosure belongs. Although methods and materials similar or equivalent to those described herein can be used in the practice of the disclosed methods and compositions, the exemplary methods, devices and materials are described herein. It is to be understood that this disclosure is not limited to particular methods, and experimental conditions described, as such methods and conditions may vary.

example1

e Biosynthesis of Methanol Using Artificially Constructed Bio-Electrode

[0111]Bacterial Culture

[0112]Three different microbial cultures, viz., Pseudomonas aeruginosa MTCC 25019, Shewanella sp. MTCC 25020, Seudomonas stutzeri MTCC 25027 were grown separately at 30° C. in a medium containing the following mineral salts (per litre): 2.5 g of NaHCO3, 0.25 g of NH4Cl, 0.06 g of NaH2PO4H2O, 0.1 g of KCl, 0.024 g of C6H5FeO7 (ferric citrate), yeast extract 0.5%; peptone 0.25% 10 ml of a vitamin mix and 10 ml of a trace mineral solution. Anaerobic conditions were achieved by flushing the media with N2:CO2 (80:20) to remove oxygen and to keep the pH of the bicarbonate buffer at pH 6.8. In log phase microbial culture was centrifuged (8000 rpm) and washed in phosphate buffer. Then the equal (wet) weight of the microbes were mixed and added into fresh media, allowed for growth and centrifuged again to separate the selective mixed microbial consortia.

[0113]Bio-Electrode Construction[0114]a) First...

example 2

Biosynthesis of Ethanol Using Artificially Constructed Bioelectrode

[0121]Bacterial Culture

[0122]Three different microbial cultures, viz., Clostridium ljungdahlii DSM 13528, Sporomusa silvacetica DSM 10669, Cupriavidus nectar DSM 529, Sporomosa ovate DSM 2662 were grown separately at 30° C. in a medium containing the following mineral salts (per litre): 2.5 g of NaHCO3, 0.25 g of NH4Cl, 0.06 g of NaH2PO4H2O, 0.1 g of KCl, 0.024 g of C6H5FeO7 (ferric citrate), 10 ml of a vitamin mix and 10 ml of a trace mineral solution. Anaerobic conditions were achieved by flushing the media with N2:CO2 (80:20) to remove oxygen and to keep the pH of the bicarbonate buffer at pH 6.8. Each microbial culture was centrifuged (8000 rpm) and washed in phosphate buffer. Then the equal (wet) weight of the microbes were mixed and added into fresh media, allowed for growth and centrifuged again to separate the selective mixed microbial consortia.

[0123]Bio-Electrode Construction[0124]a) First, the carbon felt ...

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Abstract

The present disclosure provides a ready-to-use bio-electrode stable for long term storage and a process of constructing the same. The process for construction of bio-electrode for electro-biocatalysis comprising of: selection of an electro-active bacteria; enrichment of said electro-active bacteria in a nutrient rich medium; separation of said electro-active bacterial cells from said nutrient rich medium; selection of an electrode material; surface modification of said electrode material; layering the surface modified electrode material with conductive material; layering the surface modified electrode material with an electro-active bacterial cells along with biofilm inducing agents and stabilizing agents; conditioning the electro-active bacterial cells layered electrode; incubating the electrode obtained with an immobilizing agent along with conductive material; and conditioning the electrode with micronutrients to obtain a bio-electrode.

Description

FIELD OF THE INVENTION[0001]The present disclosure relates to the field of bio-electrochemical system. In particular, the present disclosure provides a ready-to-use bio-electrode stable for long term storage and a process of constructing the same.BACKGROUND OF THE INVENTION[0002]Bio-electrochemical System (BES) relies on the interaction of electro-active microbes with electrodes. Electro-active microbes form a biofilm on the electrode and directly or indirectly transfer electrons to convert chemical energy to electricity or vice versa using inorganic / organic source of carbon. The natural formation of an electro-active biofilm, with stable current consumption / production entails prolonged conditioning periods, as long as several weeks to months, depending upon the nature of electrodes, and bacterial physiology. This long duration for production of biofilm makes the BES less attractive. Moreover, a system like microbial electro-synthesis where CO2 is converted to organic chemicals, suf...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): C25B11/04C25B11/12C12N11/02C12N1/20C25B3/00H01M8/16
CPCH01M8/16C25B11/0442C12N11/02C25B11/12B82Y30/00C25B3/00C12N1/20B82Y5/00B82Y15/00C12Q1/02C12Q1/001C25B11/073C25B11/043
Inventor KUMAR, MANOJSAHOO, PRAKASH CHANDRASANDIPAM, SRIKANTHPURI, SURESH KUMARRAMAKUMAR, SANKARA SRI VENKATA
Owner INDIAN OIL CORPORATION
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