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Tissue engineering peripheral nerve used for repairing peripheral nerve defect and its preparation method

A peripheral nerve and tissue engineering technology, applied in the field of medical biomaterials, can solve the problems of relying on the promotion of materials, ignoring the core role of seed cells and neurotrophic factors, and uncertain clinical efficacy, so as to promote nerve axon regeneration and prevent loss , the effect of protecting neurons

Inactive Publication Date: 2005-03-09
STOMATOLOGICAL HOSPITAL NO 4 ARMY MEDICAL COLLEGE PLA
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Problems solved by technology

Then there is Wang Shenguo's Chinese patent (application number 00123465.X), the main disadvantages are: (1) The core role played by seed cells and neurotrophic factors in nerve regeneration is ignored, and the promotion of nerve regeneration by materials is overly dependent , the clinical efficacy is uncertain; (2) It does not involve the combination of cells and neurotrophic factors with biomaterials

Method used

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  • Tissue engineering peripheral nerve used for repairing peripheral nerve defect and its preparation method
  • Tissue engineering peripheral nerve used for repairing peripheral nerve defect and its preparation method
  • Tissue engineering peripheral nerve used for repairing peripheral nerve defect and its preparation method

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Embodiment Construction

[0021] Now the present invention will be further described in conjunction with specific embodiments.

[0022] 1 Schwann cell culture

[0023] 10 offspring SD rats were sacrificed and disinfected with alcohol. The bilateral sciatic nerves were taken and placed on an ice-bath operating table to remove the epineurium and adhesive tissue. After adding trypsin / collagenase to digest, the upper cell suspension was collected and centrifuged. Clear, add culture medium to resuspend, inoculate in culture flask and culture. After 24 hours, 10 -5 M arabinocytes-, after 48 hours of action, they were replaced with G-418 medium containing 100 μg / ml, and cultured continuously for 5 days. Subsequently, 2 μM fluskolin was added to continue culturing, and the medium was changed every 3 days until the cells became confluent.

[0024] 2 Preparation of the nerve guide

[0025] Prepare nerve conduits with pores of 100 μm-300 μm according to the controlled solvent evaporation method or dissolved s...

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Abstract

A tissue-engineered peripheral nerve for reparing the demaged peripheral nerve is composed of neural canal and neuroglia cells or stem cells, and is prepared through preparing neural canal, preparing neurotrophic factor release controllable microspheres, and configuring the tissue-engineered peripheral nerve.

Description

technical field [0001] The invention belongs to the technical field of medical biomaterials that can be implanted into human bodies, and relates to a tissue-engineered peripheral nerve for repairing peripheral nerve defects and a preparation method thereof. Background technique [0002] For the long-distance peripheral nerve defects often encountered in clinical treatment, due to the lack of elasticity of the nerves, most cases cannot be directly anastomosed. Autologous nerve transplantation is still the most widely used method in clinical practice. However, this method has disadvantages that are difficult to overcome. After the donor nerve is excised, it will inevitably cause sensory disturbance in the donor area, and traumatic neuroma may occur, causing local pain. Additional nerve extraction will prolong the operation time and increase the scar in the donor area. Moreover, the variation in the thickness of the nerves makes the excised nerves not necessarily suitable for m...

Claims

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Application Information

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IPC IPC(8): A61F2/00A61L27/38A61L29/00C12N5/08
CPCA61L27/3834A61L27/56A61L27/58A61L2430/32
Inventor 金岩张勇杰
Owner STOMATOLOGICAL HOSPITAL NO 4 ARMY MEDICAL COLLEGE PLA
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