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Mesenchymal stem cell injection liquid and preparation method

A technology of bone marrow mesenchymal stem cells, applied in the field of bone marrow mesenchymal stem cell injection and preparation, can solve the problems of abnormal movement of facial expression muscles, transient, 3-5 months, and subcutaneous beaded knots, etc., to achieve Good differentiation ability and proliferative ability, promote wound healing, and brighten skin tone

Inactive Publication Date: 2018-03-23
南京九圣生物医学科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Botulinum toxin is a neurotoxin. It relies on paralysis of motor nerves to eliminate wrinkles, so it is easy to cause damage to nerves, resulting in abnormal movement of facial expression muscles, forming a so-called "mask face"; Re-injection is required in 5 months, causing multiple damages to the human face
Collagen is a heterogeneous protein, and the human body’s rejection reaction will reject the heterogeneous protein, so bovine collagen injection filling often results in subcutaneous beading and short-term curative effect
Surgical facelift and wrinkle removal surgery has a large scope, and there may be many complications such as infection, ecchymosis, and hair loss
[0005] To repair the scars left by damaged skin, at present, drugs and laser surgery are mainly used, which inevitably have certain risks.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Prepare L-DMEM complete culture solution, complete culture solution contains mass concentration 20% FBS, 100U / ml penicillin and 100μg / ml streptomycin;

[0041] Take the bone marrow, put it in a sterile heparin tube, add 100U heparin to each ml of bone marrow for anticoagulation, mix thoroughly, and adjust the concentration of bone marrow cells to 10 with PBS solution.6 / ml, add Ficoll separation solution and bone marrow cell suspension at a volume ratio of 1:1, without destroying the interface, centrifuge at 500g for 25 minutes at 20°C in a horizontal centrifuge, settle naturally, take out mononuclear cells from the interface, and wash with PBS 2 times;

[0042] Add the obtained cells to the complete culture medium of L-DMEM for cell counting, reaching 10 7 / ml after primary culture, press 1×10 6 cells / ml were inoculated into T25 cell culture flasks, the volume of complete culture solution in each cell culture flask was 5 ml, placed in CO 2 Culture in a 37°C incubator...

Embodiment 2

[0055] Prepare L-DMEM complete culture solution, complete culture solution contains mass concentration 20% FBS, 100U / ml penicillin and 100μg / ml streptomycin;

[0056] Take the bone marrow, put it in a sterile heparin tube, add 100U heparin to each ml of bone marrow for anticoagulation, mix thoroughly, and adjust the concentration of bone marrow cells to 10 with PBS solution. 7 / ml, add Ficoll separation solution and bone marrow cell suspension at a volume ratio of 1:1, without destroying the interface, centrifuge at 500g for 30 minutes at 20°C in a horizontal centrifuge, settle naturally, take out mononuclear cells from the interface, and wash with PBS 3 times;

[0057] Add the obtained cells to the complete culture medium of L-DMEM for cell counting, reaching 10 6 / ml after primary culture, press 3×10 6 cells / ml was inoculated into T75 cell culture flasks, the volume of complete culture solution in each cell culture flask was 20 ml, and placed in CO 2 Culture in a 37°C inc...

Embodiment 3

[0070] Prepare L-DMEM complete culture solution, complete culture solution contains mass concentration 20% FBS, 100U / ml penicillin and 100μg / ml streptomycin;

[0071] Take the bone marrow, put it in a sterile heparin tube, add 100U heparin to each ml of bone marrow for anticoagulation, mix thoroughly, and adjust the concentration of bone marrow cells to 10 with PBS solution. 6 / ml, add Ficoll separation solution and bone marrow cell suspension at a volume ratio of 1:1, without destroying the interface, centrifuge at 500g for 28 minutes at 20°C in a horizontal centrifuge, settle naturally, take out mononuclear cells from the interface, and wash with PBS 2 times;

[0072] Add the obtained cells to the complete culture medium of L-DMEM for cell counting, reaching 10 7 / ml after primary culture, according to 5×10 6 cells / ml was inoculated into T75 cell culture flasks, the volume of complete culture solution in each cell culture flask was 15 ml, and placed in CO 2 Culture in a 3...

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Abstract

The invention provides mesenchymal stem cell injection liquid and a preparation method, which aim to solve the problem of how to use a mesenchymal stem cell to efficiently repair and improve damaged skin and fundamentally delay and stop skin cell aging. The injection liquid provided by the invention is prepared from the mesenchymal stem cell, DMSO, EGF, glutathione, vitamin C, human serum albumin,a ginkgo biloba extract and a menstruum. The mesenchymal stem cell is obtained through carrying out hungry culture on P2 to P5-generation mesenchymal stem cells and stimulating through an epidermal growth factor. The obtained mesenchymal stem cell has a good differentiative capacity and a good proliferation capacity, has a better differentiation potential, and is synergized with the DMSO, the EGF, the glutathione, the vitamin C, the human serum albumin and the ginkgo biloba extract, so that skin wrinkles and hyperpigmentation can be remarkably reduced.

Description

technical field [0001] The invention relates to the field of stem cells, in particular to a bone marrow mesenchymal stem cell injection and a preparation method thereof. Background technique [0002] With the gradual improvement of people's material living standards, the affluence of life makes people begin to pursue a higher level of life. Everyone has a desire for beauty, and everyone hopes to be young and beautiful forever. As the largest organ of the human body, the skin covers the appearance of the human body and is the main organ of aesthetic beauty research. How to improve skin quality, repair Damaged and aging skin has become a major concern in aesthetic cosmetology research. [0003] How to delay and prevent the aging of skin cells is not only the fundamental issue of beauty and anti-aging, but also the key to skin care. With age, the ability of basal cells to divide gradually decreases, the level of skin growth factors decreases, and the effect weakens, apoptosis...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/38A61K35/28A61K9/08C12N5/0775A61P17/18A61P17/16A61K31/375A61K31/10
CPCA61K9/0019A61K9/08A61K31/10A61K31/375A61K35/28A61K36/16A61K38/06A61K38/1808A61K38/385A61K47/02A61K47/26C12N5/0663C12N2501/11C12N2501/91A61K2300/00
Inventor 祁守岗胡建华王晶晶程琴
Owner 南京九圣生物医学科技股份有限公司
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