Hydrogel for intelligent desorption of cell sheet layer and application of hydrogel

A hydrogel and cell sheet technology, which is applied in the fields of biomedical engineering and intelligent polymer materials, can solve the problems of non-biocompatible polymers and cell sheet desorption technology, and achieve good application prospects, simple ingredients, The effect of easy operation

Active Publication Date: 2012-10-10
TIANJIN CHANGHE BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology allows for quicker growth and separation of biological material from living organisms without damaging them during processing. It also provides an environmentally friendly solution where it prevents harmful chemical reactions between certain substances inside the body while preserving their structure.

Problems solved by technology

Technologies described in this patents involve combining different types of chemical compounds together to create an artificially made solution containing multiple layers of these chemistry elements. These solutions may contain various ingredients like drugs or other agents to improve their effectiveness during treatments. They also include carriages made up of polyethylenimine(PEI), polytetrafluorocyanoacrilum nitrogen responsion technique, microsponge techniques, and membrane technics involving suspending cells within liquid droplets. By controllably adjusting certain physical parameters, they allow for effective use of therapeutic compositions and delivery systems.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A smart detachment hydrogel for cell sheets, composed of N-isopropylacrylamide, N,N'-methylenebisacrylamide and sodium carboxymethylcellulose dissolved in deionized water The methylethylenediamine initiation system is formed by radical polymerization, and the mass percentage of the isopropylacrylamide, N, N'-methylenebisacrylamide, carboxymethylcellulose sodium and deionized water is 10% %, 2%, 8%, 80%, reduce the hydrogel environment temperature to 5°C, and the dermal fibroblasts proliferating for 7 days are desorbed from the surface of the hydrogel to form a sheet structure. The desorbed skin fibroblast sheet can be used for the treatment and repair of burnt skin epidermis.

Embodiment 2

[0027] A kind of cell sheet intelligent desorption hydrogel, which is formed by free radical polymerization by dissolving N-isopropylacrylamide, hectorite and sodium alginate in deionized water and using ammonium persulfate / tetramethylethylenediamine initiation system into, the mass percent of the isopropylacrylamide, hectorite, sodium alginate and deionized water is 11%, 1%, 3%, 85%, and the ambient temperature of the hydrogel is reduced to 4°C, and the proliferation is 7 Day corneal cells detach from the hydrogel surface to form a sheet structure. The detached corneal cell sheet can be used for the treatment and repair of the cornea.

Embodiment 3

[0029] A cell sheet intelligent desorption hydrogel, composed of N,N-dimethylaminoethyl methacrylate, ethylene glycol dimethacrylate and sodium humate dissolved in deionized water using ammonium persulfate / tetramethyl The base ethylenediamine initiation system carries out radical polymerization, and the mass percent of described N,N-dimethylaminoethyl methacrylate, ethylene glycol dimethacrylate, sodium humate and deionized water is 8 %, 3%, 4%, 85%, lower the ambient temperature of the hydrogel to 3°C, and the bone marrow mesenchymal stem cells proliferated for 7 days detach from the surface of the hydrogel to form a sheet structure. The desorbed bone marrow mesenchymal stem cell sheet can be used for the treatment and repair of scleroderma.

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Abstract

The invention relates to hydrogel for intelligent desorption of a cell sheet layer and an application of the hydrogel. The hydrogel is formed by dissolving functional monomers M1, chemical or physical crosslinking agents M2 and water-solubility biomacromolecules M3 in solvents 4 and adopting an oxidation reduction initiating system for free radical polymerization, and the mass percentages of M1, M2, M3 and M4 are respectively 5 to 10 percent, 2 to 10 percent, 1 to 15 percent and 75 to 92 percent. The hydrogel has excellent temperature or pH value response characteristics and biocompatibility, and the fast multiplication and desorption of cells can be effectively promoted. The cell sheet layer formed after the desorption maintains the complete cell epimatrix, and the biological function damage of the cells caused by an enzyme hydrolysis method and the cell damage caused by degradation ingredients of support frame materials are avoided. The cell sheet layer after the intelligent desorption by the hydrogel can be used for treating and repairing relevant tissues and organs of skin, corneal epithelium and parodontium ligament.

Description

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Claims

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Application Information

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Owner TIANJIN CHANGHE BIOLOGICAL TECH
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