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Coated micro capsule of lactic acid bacteria and its preparation

A technology of lactic acid bacteria microcapsules and lactic acid bacteria, which is applied in the direction of microcapsules, capsule delivery, and pharmaceutical formulations. It can solve the problems of intolerant feed high temperature, inability to release rapidly, and low activity of lactic acid bacteria, and achieve enhanced protection and heat resistance. Sexuality, good protective effect

Inactive Publication Date: 2005-01-26
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the low activity of lactic acid bacteria in the lactic acid bacteria capsules prepared by the prior art, which cannot be released rapidly in the intestinal tract, and cannot tolerate the trace element metal ions, choline chloride, antibiotics, etc. contained in the feed. The damage of various harmful factors, the inability to withstand the high temperature in the process of feed pelleting, and the defects of high cost, so as to provide a kind of food with high activity, storage resistance, high stability, low cost, which can pass through gastric juice safely and enter Lactic acid bacteria microcapsules that rapidly disintegrate post-intestinal

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Use a culture medium, which includes components A, B, C and D, wherein the component A is one of beef extract or peptone, yeast extract, skimmed milk / powder, soybean peptide, tryptone, soybean powder or more mixtures; the component B is a mixture of one or more of lactose, glucose, tomato juice, malt extract; the component C is Tween-80, cysteine, carrot juice, potato juice, green onion juice, mushroom juice in one or more mixtures; the component D is NaCl, KH 2 PO 4 , diammonium citrate, sodium acetate, MgSO 4 For a mixture of one or more of them, sterilize the culture medium at 121°C for 15-30 minutes, and cool to room temperature.

[0027] Isolate a strain of lactic acid bacteria (such as Lactobacillus plantarum) from the intestinal tract of a healthy animal, insert it into the above medium, and cultivate it at 25-42°C for 12 hours; centrifuge the culture solution at 4000r / min for 30min to obtain the lactic acid bacteria sludge Dilute the lactic acid bacteria slim...

Embodiment 2

[0031] A strain of lactic acid bacteria (such as Streptococcus faecalis) was isolated from the intestinal tract of a healthy animal, inserted into the culture medium described in Example 1, and cultivated at 25-42° C. for 12 hours; Centrifuge for 30 minutes to obtain lactic acid bacteria slime; dilute the lactic acid bacteria slime with sterilized physiological saline, add skimmed milk powder with 3% total weight of lactic acid bacteria slime, and obtain a live bacteria concentration of 10 9 cfu / ml lactic acid bacteria concentrate.

[0032] Adopt the FLP type fluidized granulation coating drier, put microcrystalline cellulose: sucrose: cornstarch=2: 3: 5 (weight ratio) the pill-making auxiliary material 2kg that forms into pellet-making pot, spray into above-mentioned lactic acid bacteria simultaneously Concentrate, the weight ratio of pelleting auxiliary materials and lactic acid bacteria concentrate is 1: 2, and spray twice; the process conditions of the fluidized granulatio...

Embodiment 3

[0035] A strain of lactic acid bacteria (such as Lactobacillus acidophilus, and of course Lactobacillus fermentum or Lactobacillus casei) was isolated from the intestinal tract of a healthy animal, inserted into the medium described in Example 1, and cultivated at 25-42°C 12 hours; under the condition of 4000r / min, the culture solution was centrifuged for 30min to obtain the lactic acid bacteria sludge; the lactic acid bacteria sludge was diluted with sterilized physiological saline, and the skimmed milk powder with 10% of the total weight of the lactic acid bacteria sludge was added to obtain the live bacteria concentration of 10 11 cfu / ml lactic acid bacteria concentrate.

[0036] Adopt FLP type fluidized granulation coating dryer, according to sucrose: maltodextrin: protein sugar = 2: 3: 4 (weight ratio) the pill-making auxiliary material 2kg that forms is dropped in the pill-making pot, sprays into above-mentioned lactic acid bacteria concentration simultaneously Liquid, ...

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Abstract

The invention discloses a micro capsule of lactic acid bacteria and its preparation, wherein the micro capsule includes a core material of lactic acid bacterium particles, the bolus making auxiliary material being one or more selected from cane sugar, maltose dextrin, crystalline cellulose, maize starch, glucose or protein sugar, and the wall material comprises sodium acetylide and CaCl2, carrageenan, soybean separated albumen powder, and water-insoluble plant oil.

Description

field of invention [0001] The invention relates to an active microorganism, in particular to a microcapsule coated with lactic acid bacteria and a preparation method thereof. Background technique [0002] Antibiotics once played an important role in livestock and poultry farming, but the disadvantages such as the spread of drug resistance, drug residues and ecological damage have become more and more obvious. With the strengthening of people's food safety awareness, it is urgent to use green feed additives to produce green animal products. Lactic acid bacteria microbial feed additive is one of the very important ones. Lactic acid bacteria are resident beneficial bacteria in animals, which can resist pathogenic microorganisms, regulate the balance of animal digestive tract microflora; activate the immune system and enhance immunity; in addition, lactic acid bacteria can also prevent livestock diarrhea and increase livestock weight gain , plays an important physiological fun...

Claims

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Application Information

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IPC IPC(8): A61K9/50A61K35/744A61K35/747A61K47/42A61K47/46A61P31/00A61P37/04
Inventor 张日俊刘丽英
Owner CHINA AGRI UNIV
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