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Coated microcapsule of lactic acid bacteria and its preparation method

A lactic acid bacteria microcapsule, lactic acid bacteria technology, applied in the application, animal feed, animal feed and other directions, can solve the problems of intolerant of high temperature of feed, inability to release quickly, low activity of lactic acid bacteria, etc., to enhance the protection effect, enhance heat resistance, good protective effect

Inactive Publication Date: 2014-04-30
陈双露
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to overcome the low activity of lactic acid bacteria in the lactic acid bacteria capsules prepared by the prior art, which cannot be released rapidly in the intestinal tract, and cannot tolerate the trace element metal ions, choline chloride, antibiotics, etc. contained in the feed. The damage of various harmful factors, the inability to withstand the high temperature in the process of feed pelleting, and the defects of high cost,

Method used

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  • Coated microcapsule of lactic acid bacteria and its preparation method
  • Coated microcapsule of lactic acid bacteria and its preparation method
  • Coated microcapsule of lactic acid bacteria and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Use a culture medium, which includes components A, B, C and D, wherein the component A is one of beef extract or peptone, yeast extract, skimmed milk / powder, soybean peptide, tryptone, soybean powder or more mixtures; the component B is a mixture of one or more of lactose, glucose, tomato juice, malt extract; the component C is Tween-80, cysteine, carrot juice, potato One or more mixtures in juice, scallion juice, oyster mushroom juice; the component D is one or more mixtures of NaCI, KH2PO4, diammonium citrate, sodium acetate, MgSO4, the culture medium Sterilize at 121°C for 15-30 minutes, and cool to room temperature.

[0030] A strain of lactic acid bacteria (such as Lactobacillus plantarum) is isolated from the intestines of healthy animals, inserted into the above medium, and cultivated at 25-42°C for 12 hours; the culture solution is centrifuged at 4000r / min for 30min to obtain the lactic acid bacteria mud;

[0031] Dilute the lactic acid bacteria slime with ste...

Embodiment 2

[0035] Isolate a strain of lactic acid bacteria (such as Streptococcus faecalis) from the intestinal tract of healthy animals, insert it into the medium described in Example 1, and cultivate it at 25~42°C for 12 hours; , and centrifuged for 30min to obtain the lactic acid bacteria slime; dilute the lactic acid bacteria slime with sterilized physiological saline, add skim milk powder with 3% total weight of the lactic acid bacteria slime, and obtain a live bacteria concentration of 10 9 cfu / mL lactic acid bacteria concentrate.

[0036] Adopt FLP type fluidized granulation coating drier, put microcrystalline cellulose: sucrose: cornstarch: 2: 3: 5 (weight ratio) the pill-making auxiliary material 2kg that forms into pill-making pot, spray into above-mentioned lactic acid bacteria at the same time Concentrate, the weight ratio of pelleting auxiliary materials and lactic acid bacteria concentrate is 1: 2, and spray twice; the process conditions of the fluidized granulation coating...

Embodiment 3

[0040] A strain of lactic acid bacteria (such as Lactobacillus acidophilus, and of course Lactobacillus fermentum or Lactobacillus casei) was isolated from the intestinal tract of a healthy animal, inserted into the medium described in Example 1, and cultivated at 25-42°C 12 hours; under the condition of 4000r / min, the culture solution was centrifuged for 30min to obtain the lactic acid bacteria sludge; the lactic acid bacteria sludge was diluted with sterilized physiological saline, and the skimmed milk powder with 10% of the total weight of the lactic acid bacteria sludge was added to obtain the live bacteria concentration of 10 11 CFU of lactic acid bacteria concentrate.

[0041] Adopt FLP type fluidized granulation coating dryer, according to sucrose: maltodextrin: proteoglycan: 2:3:4 (weight ratio) the pill-making auxiliary material 2kS puts into the pill-making pot, sprays into above-mentioned lactic acid bacteria concentration at the same time liquid, the weight ratio ...

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Abstract

The invention relates to a coated microcapsule of lactic acid bacteria and its preparation method. The microcapsule includes: a core material of lactic acid bacterium particles; a pelleting auxiliary material selected from one or a mixture of the followings: cane sugar, maltodextrin, microcrystalline cellulose, corn starch, glucose and glycoprotein; and a wall material comprises sodium alginate and CaCl2, and further comprises one, two or three of carrageenan, soybean protein isolate, and water-insoluble plant oil. The lactic acid bacteria microcapsule by lactic acid bacteria is prepared by mixing lactic acid bacteria with the pelleting material, granulating, and coating the mixture with an enteric dressing material. The lactic acid bacteria microcapsule employs protection on lactic acid bacteria, so that lactic acid bacteria can safely pass through the gastric juice, enter into the intestine and rapidly disintegrate in the intestine to occupy the planting sites, thereby inhibiting the growth of pathogens. The lactic acid bacteria microcapsule has good storage stability, tolerance to metal ions and tolerance the high temperature at normal temperature, and good usage effect in the production of livestock and poultry.

Description

field of invention [0001] The invention relates to an active microorganism, in particular to a microcapsule coated with lactic acid bacteria and a preparation method thereof. Background technique [0002] Antibiotics once played an important role in livestock and poultry farming, but the disadvantages such as the spread of drug resistance, drug residues and ecological damage have become more and more obvious. With the strengthening of people's food safety awareness, there is an urgent need to use green feed additives to produce green livestock crystals. Lactic acid bacteria microbial feed additive is one of the very important ones. Lactic acid bacteria are resident beneficial bacteria in animals, which can resist pathogenic microorganisms, regulate the balance of animal digestive tract microflora; activate the immune system and enhance immunity; in addition, lactic acid bacteria can also prevent livestock diarrhea and increase livestock weight gain , plays an important phy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/16
Inventor 陈双露
Owner 陈双露
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