Compound microorganism preparation used for aquiculture water body improvement and preparation method of compound microorganism preparation

A technology for compound microorganisms and water body improvement, which is applied in the field of compound microorganism preparations for the improvement of aquaculture water bodies and the preparation thereof, can solve the problems of high cost and complicated preparation, and achieves the effects of low cost, simple preparation method and BOD reduction.

Active Publication Date: 2013-10-16
SHANGHAI CHUANGBO ECOLOGICAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] Although microbial products have been used in aquaculture, there are still problems such as complica

Method used

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  • Compound microorganism preparation used for aquiculture water body improvement and preparation method of compound microorganism preparation
  • Compound microorganism preparation used for aquiculture water body improvement and preparation method of compound microorganism preparation
  • Compound microorganism preparation used for aquiculture water body improvement and preparation method of compound microorganism preparation

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0048] Example 1

[0049] Raw material composition:

[0050]

[0051] Preparation:

[0052] step 1,

[0053] Bacillus subtilis and Bacillus amyloliquefaciens were respectively inoculated into the first medium under aseptic conditions at 30°C-35°C and cultured for 48 hours with a single strain to obtain seed culture solutions of Bacillus subtilis and Bacillus amyloliquefaciens. The number of viable bacteria is greater than 5.0×10 9 cfu / ml. Among them, the first medium consists of molasses 1-1.2wt%, peptone 0.4-0.6wt%, yeast extract 1-1.2wt%, calcium chloride 0.1-0.15wt%, sodium chloride 0.2-0.25wt%, iron sulfate 30 -35wt%, magnesium sulfate 0.02-0.03wt%, growth factor solution 1-1.2wt% (including vitamin H2-2.3wt%, vitamin B12-15wt% aqueous solution) and the balance of water, the pH value is 6.5-6.8 .

[0054] Inoculate Thiobacillus denitrificans in the second medium under aseptic conditions at a light intensity of 4500-10000lx and a temperature of 29°C-33°C for 6-8 days to obtain se...

Example Embodiment

[0064] Example 2

[0065] Raw material composition:

[0066]

[0067] Preparation:

[0068] step 1,

[0069] According to the method described in Example 1, the Bacillus subtilis seed culture solution, the Bacillus amyloliquefaciens seed culture solution, and the Thiobacillus denitrificans seed culture solution are provided.

[0070] Step 2.

[0071] According to the method described in Example 1, a concentrated bacterial solution with a high concentration was obtained as the mother liquor.

[0072] Step 3.

[0073] The concentrated bacterial liquid is transferred into a matrix composed of zeolite powder + calcium carbonate for feed + corn starch + molasses and mixed, and the mixed fluid is granulated on a swing machine, and then boiled and dried. The moisture mass percentage after drying is 5-7%.

[0074] Among them, based on the total weight of the substrate (carrier and nutrients), the substrate is composed of 75% by weight of zeolite powder, 10% by weight of calcium carbonate, 8% by wei...

Example Embodiment

[0077] Example 3

[0078] Raw material composition:

[0079]

[0080] Preparation:

[0081] step 1,

[0082] According to the method described in Example 1, the Bacillus subtilis seed culture solution, the Bacillus amyloliquefaciens seed culture solution, and the Thiobacillus denitrificans seed culture solution are provided.

[0083] Step 2.

[0084] According to the method described in Example 1, a concentrated bacterial solution with a high concentration was obtained as the mother liquor.

[0085] Step 3.

[0086] The concentrated bacterial liquid is transferred into a matrix composed of zeolite powder + calcium carbonate for feed + corn starch + molasses and mixed, and the mixed fluid is granulated on a swing machine, and then boiled and dried. The moisture mass percentage after drying is 5-7%.

[0087] Among them, based on the total weight of the substrate (carrier and nutrients), the substrate is composed of 65 wt% of zeolite powder, 15 wt% of calcium carbonate, 5 wt% of corn starch, a...

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Abstract

The invention provides a compound microorganism preparation used for freshwater aquiculture water body improvement, which comprises the raw materials by weight percentage: 25-30wt% of bacillus subtilis, 25-30wt% of bacillus amyloliquefaciens and 15-20wt% of thiobacillus denitrificans. The invention further provides a preparation method of the microorganism preparation, which comprises the steps that bacillus subtilis, bacillus amyloliquefaciens and thiobacillus denitrificans seed culture fluids are sequentially inoculated into a culture medium and fermented; an obtained bacterium fluid is mixed with a carrier and a nutritional substance; and the microorganism preparation is obtained. The microorganism preparation can obviously improve the water quality of an aquiculture water body, and can improve the meat quality of an aquiculture animal.

Description

technical field [0001] The invention relates to the technical field of water body improvement, in particular to a compound microbial preparation for water body improvement in aquaculture and a preparation method thereof. Background technique [0002] China is the world's largest aquaculture country. According to statistics, China's inland aquaculture water area is 27.34 million hectares, seawater aquaculture area is 4.38 million hectares, and rice field fish farming area is 1.52 million hectares in 2001. Especially in the water towns in southern China, aquaculture plays an important role in agricultural production. Many areas regard aquaculture as an important way for local farmers to get rich. Aquaculture has gradually become one of the pillar industries in aquaculture agriculture. [0003] At the same time, China is also one of the countries with the most serious pollution of aquaculture water. Since ancient times, there has been a saying in China that "raise fish first b...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/07C12R1/125C12R1/01
Inventor 江瀚陈仁爱
Owner SHANGHAI CHUANGBO ECOLOGICAL ENG
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