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Compound microorganism preparation used for aquiculture water body improvement and preparation method of compound microorganism preparation

A technology for compound microorganisms and water body improvement, which is applied in the field of compound microorganism preparations for the improvement of aquaculture water bodies and the preparation thereof, can solve the problems of high cost and complicated preparation, and achieves the effects of low cost, simple preparation method and BOD reduction.

Active Publication Date: 2013-10-16
SHANGHAI CHUANGBO ECOLOGICAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] Although microbial products have been used in aquaculture, there are still problems such as complicated preparation and high cost, and new microbial preparations for aquaculture water improvement are needed.

Method used

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  • Compound microorganism preparation used for aquiculture water body improvement and preparation method of compound microorganism preparation
  • Compound microorganism preparation used for aquiculture water body improvement and preparation method of compound microorganism preparation
  • Compound microorganism preparation used for aquiculture water body improvement and preparation method of compound microorganism preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Raw material composition:

[0050]

[0051] Preparation:

[0052] step 1,

[0053] Bacillus subtilis and Bacillus amyloliquefaciens were respectively inoculated in the first culture medium under aseptic conditions, 30 DEG C-35 DEG C, single bacterial species was cultivated for 48 hours, obtained Bacillus subtilis and Bacillus amyloliquefaciens seed culture solution, which The number of live bacteria is greater than 5.0×10 9 cfu / ml. Among them, the first medium is composed of molasses 1-1.2wt%, peptone 0.4-0.6wt%, yeast extract 1-1.2wt%, calcium chloride 0.1-0.15wt%, sodium chloride 0.2-0.25wt%, iron sulfate 30 -35wt%, magnesium sulfate 0.02-0.03wt%, growth factor solution 1-1.2wt% (including vitamin H2-2.3wt%, vitamin B12-15wt% aqueous solution) and the rest of water, the pH value is 6.5-6.8 .

[0054] Inoculate Thiobacillus denitrificans in the second culture medium under aseptic conditions, cultivate for 6-8 days under the conditions of light intensity of 4500...

Embodiment 2

[0065] Raw material composition:

[0066]

[0067] Preparation:

[0068] step 1,

[0069] According to the method described in Example 1, the seed culture liquid of Bacillus subtilis, the seed culture liquid of Bacillus amyloliquefaciens, and the seed culture liquid of Thiobacillus denitrification were provided.

[0070] Step 2,

[0071] According to the method described in Example 1, a high-concentration concentrated bacterial solution was obtained as a mother solution.

[0072] Step 3,

[0073] Move the concentrated bacterial liquid into the matrix composed of zeolite powder + calcium carbonate for feed + cornstarch + molasses and mix. The mixed fluid is granulated on a swing machine, and then boiled and dried. The mass percentage of moisture after drying is 5-7%.

[0074] Wherein, based on the total weight of the matrix (carrier and nutrients), the matrix is ​​composed of 75wt% zeolite powder, 10wt% calcium carbonate, 8wt% cornstarch, and 7wt% molasses.

[0075] Ste...

Embodiment 3

[0078] Raw material composition:

[0079]

[0080]Preparation:

[0081] step 1,

[0082] According to the method described in Example 1, the seed culture liquid of Bacillus subtilis, the seed culture liquid of Bacillus amyloliquefaciens, and the seed culture liquid of Thiobacillus denitrification were provided.

[0083] Step 2,

[0084] According to the method described in Example 1, a high-concentration concentrated bacterial solution was obtained as a mother solution.

[0085] Step 3,

[0086] Move the concentrated bacterial liquid into the matrix composed of zeolite powder + calcium carbonate for feed + cornstarch + molasses and mix. The mixed fluid is granulated on a swing machine, and then boiled and dried. The mass percentage of moisture after drying is 5-7%.

[0087] Wherein, based on the total weight of the matrix (carrier and nutrients), the matrix is ​​composed of 65wt% zeolite powder, 15wt% calcium carbonate, 5wt% cornstarch and 5wt% molasses.

[0088] Step ...

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Abstract

The invention provides a compound microorganism preparation used for freshwater aquiculture water body improvement, which comprises the raw materials by weight percentage: 25-30wt% of bacillus subtilis, 25-30wt% of bacillus amyloliquefaciens and 15-20wt% of thiobacillus denitrificans. The invention further provides a preparation method of the microorganism preparation, which comprises the steps that bacillus subtilis, bacillus amyloliquefaciens and thiobacillus denitrificans seed culture fluids are sequentially inoculated into a culture medium and fermented; an obtained bacterium fluid is mixed with a carrier and a nutritional substance; and the microorganism preparation is obtained. The microorganism preparation can obviously improve the water quality of an aquiculture water body, and can improve the meat quality of an aquiculture animal.

Description

technical field [0001] The invention relates to the technical field of water body improvement, in particular to a compound microbial preparation for water body improvement in aquaculture and a preparation method thereof. Background technique [0002] China is the world's largest aquaculture country. According to statistics, China's inland aquaculture water area is 27.34 million hectares, seawater aquaculture area is 4.38 million hectares, and rice field fish farming area is 1.52 million hectares in 2001. Especially in the water towns in southern China, aquaculture plays an important role in agricultural production. Many areas regard aquaculture as an important way for local farmers to get rich. Aquaculture has gradually become one of the pillar industries in aquaculture agriculture. [0003] At the same time, China is also one of the countries with the most serious pollution of aquaculture water. Since ancient times, there has been a saying in China that "raise fish first b...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/07C12R1/125C12R1/01
Inventor 江瀚陈仁爱
Owner SHANGHAI CHUANGBO ECOLOGICAL ENG
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