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Preparation method for composite bacillus microcapsule preparations

A technology of compounding Bacillus cereus and Bacillus cereus is applied in the field of microbial preparations for aquatic animals, which can solve the problems of limited effect of aquatic animals, and achieve the effects of enhancing nutritional immunity, long storage time, and inhibiting synthesis.

Inactive Publication Date: 2015-09-23
TONGWEI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to different strains and genera, there are certain differences in their physiological and biochemical characteristics and metabolites, and they are highly dependent on the growth environment and host. It can be seen that the Bacillus subtilis preparation of this application is a microcapsule preparation and is a veterinary subtilis Bacillus preparations, therefore, have extremely limited effects on aquatic animals

Method used

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  • Preparation method for composite bacillus microcapsule preparations
  • Preparation method for composite bacillus microcapsule preparations

Examples

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Effect test

Embodiment 1

[0027] A preparation method for a composite bacillus microcapsule preparation, comprising the following steps: (1) respectively separating Bacillus subtilis YB-1 CGMCC No.5116, Bacillus cereus YB-2 CGMCC No.5117 and Bacillus cereus YB-2 CGMCC No. Bacillus flexus XH-1 CGMCC No.5115, add water to make a bacterial suspension, then mix the above three bacterial suspensions of Bacillus subtilis and inoculate them into the seed culture solution to ferment to prepare a probiotic seed solution, and then make the probiotic seed solution The obtained probiotic seed liquid is inoculated into the fermentation culture liquid and fermented to obtain a fermentation product;

[0028] (2) Add protective agent to the fermentation product prepared in step (1), then add 1 times the volume of probiotic carrier glue, shake well and spray on CaCl 2 Immobilization treatment in the solidified liquid, after solidification, filtration and low-temperature freeze-drying to obtain freeze-dried powder, addi...

Embodiment 2

[0030]A preparation method for a composite bacillus microcapsule preparation, comprising the following steps: (1) respectively separating Bacillus subtilis YB-1 CGMCC No.5116, Bacillus cereus YB-2 CGMCC No.5117 and Bacillus cereus YB-2 CGMCC No. Bacillus flexus XH-1 CGMCC No.5115, add water to make a bacterial suspension, then mix the bacterial suspensions of the above three Bacillus subtilis and inoculate them into the seed culture solution by 3% of the volume of the seed culture solution. Ferment and culture at 25°C for 24 hours to prepare the probiotic seed liquid, and then inoculate the prepared probiotic seed liquid into the fermentation culture liquid at 3% of the volume of the fermentation culture liquid. Ferment in a closed fermentation reactor for 24h to obtain a fermentation product; the above-mentioned bacterial suspension is mixed in a ratio of 1:1:2 in the bacterial suspension; the composition of the seed culture solution includes corn flour, bean cake powder and w...

Embodiment 3

[0033] A preparation method for a composite bacillus microcapsule preparation, comprising the following steps: (1) respectively separating Bacillus subtilis YB-1 CGMCC No.5116, Bacillus cereus YB-2 CGMCC No.5117 and Bacillus cereus YB-2 CGMCC No. Bacillus flexus XH-1 CGMCC No.5115, add water to make a bacterial suspension, then mix the bacterial suspensions of the above three Bacillus subtilis and inoculate them into the seed culture solution by 3% of the volume of the seed culture solution. Ferment and culture at 35°C for 32 hours to prepare probiotic seed liquid, and then inoculate the prepared probiotic seed liquid into the fermentation culture liquid according to 4% of the volume of the fermentation culture liquid. Fermentation 35h obtains fermented product in the fermentation reactor; Above-mentioned bacterium suspension mixes with the ratio of 1:2:2 of bacteria number in the bacterium suspension; The composition of described seed culture liquid comprises corn flour, bean ...

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Abstract

The invention provides a preparation method for composite bacillus microcapsule preparations. The method comprises the steps that bacillus subtilis YB-1 CGMCC No.5116 bacterial suspension, bacillus cereus YB-2 CGMCC No.5117 bacterial suspension and bacillus flexus XH-1 CGMCC No.5115 bacterial suspension are mixed and fermented to obtain fermentation products; protective agents and bacteria carrier fluid are added into the fermentation products, the mixture is fully shaken and sprayed in CaCl2 curing liquid to conduct curing, after curing is conducted, filtering, low temperature freeze drying, adsorption carrier absorbing and drying are conducted in sequence, and the composite bacillus microcapsule preparations are obtained. The composite bacillus microcapsule preparations have the advantages that the microbial content is high, intimidation of high temperature, oxygen, acid environment on composite bacilli can be effectively avoided, and the application effect of the composite bacilli in the aquatic animal breeding process is greatly improved.

Description

technical field [0001] The invention relates to the technical field of microbial preparations for aquatic animals, in particular to a preparation method of a compound bacillus microcapsule preparation. Background technique [0002] Aquatic micro-ecological preparations are live bacterial preparations formed by using beneficial microorganisms in aquatic animals or in aquaculture water through special processing techniques. It can be used to regulate the balance of flora in the intestinal tract of animals and enhance the nutrition and immunity of aquatic animals. In aquaculture, commonly used probiotics mainly include Bacillus, photosynthetic bacteria, lactic acid bacteria, yeast and nitrifying bacteria. Bacillus has strong stress resistance, high temperature resistance, acid and alkali resistance, extrusion resistance, can be processed into pellet feed without changing activity, can regulate intestinal flora, maintain micro-ecological balance, and inhibit Vibrio in intestina...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C12N11/10C12N11/04A23K1/18C12R1/125C12R1/085C12R1/07
Inventor 李建光高启平李云兰帅柯邓玉平陈德军
Owner TONGWEI
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