Composite microbial inoculum for preparing biological bacterial fertilizer and preparation method thereof
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A technology of compound bacterial agent and biological bacterial fertilizer, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of increasing production and non-point source pollution of waste.
Inactive Publication Date: 2010-11-24
于和军
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[0003] The object of the present invention is to provide a kind of composite bacterial agent and its preparation method for preparing biological bacterial fertilizer, when utilizing this composite bacterial agent to prepare biological bacterial fertilizer, crude fiber and other nutrients in straw and poultry manure can be effectively Decomposed and released, the prepared bio-organic fertilizer can condition the soil, activate the microbial activity rate in the soil, overcome soil compaction, increase soil air permeability, increase production, and effectively solve rural agricultural waste non-point source pollution difficult problem
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Embodiment 1
[0033] Example 1: Rhodopseudomonas palustris (Rhodop seudanonas palustris) with the number 1.2352 and Candida utilis with the number 2.1180 disclosed in the strain catalog were purchased from China General Microorganism Culture Collection Center (CGMCC) (Candida utilis), Lactobacillus acidophilus (Lactobacillus acidophilus) number 1.1854, Bacillus subtilis (Bacillus subtilis) number 1.1630 and Streptomyces microflavus var. lactosus);
[0034] Step 1, the purification culture of various bacteria, the medium used, the culture temperature and the time of culture are respectively as follows:
[0035] (1) The culture medium for Rhodopseudomonas palustris is 0259ATYP agar medium, medium formula: KH 2 PO 4 1.0g, CaCl 2 0.1g, NaHCO 3 3.0g, sodium acetate 1.0g, NH 4 Cl 2 0.5g, NaCl 1.0g, trace element solution 1.0ml and vitamin solution 1.0ml;
[0036] Among them, trace element solution: FeCl 2 4H 2 O 1.8g 、CoCl2 6H 2 O 0.25g, NiCl 2 ·6H 2 O CuCl 2H 2 O 0.01g, MnC...
Embodiment 2
[0053] Example 2, using the composite microbial agent obtained in Example 1 to prepare bio-organic fertilizer, prepare 200 kg of lignite, 500 kg of poultry manure, 300 kg of straw powder, and 3 kg of composite bacterial agent.
[0054] Follow the steps below to produce:
[0055] (1) Mix 200kg of lignite, 500kg of poultry manure, and 300kg of straw powder evenly, add 3kg of compound bacterial fertilizer during the stirring process, and ferment in the fermentation workshop for 48 hours;
[0056] (2) When the temperature rises to 72°C, use the automatic compost turning machine to start composting, and in the next 8 days, turn the compost every 12 hours;
[0057] (3) During the turning process, the high temperature generated by microbial fermentation evaporates the water, and aerobic fermentation is carried out at the same time. When the water content in the compost drops to 20%, the product inspection is carried out;
[0058] (4) Quantitative packaging is done for products that ...
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Abstract
The invention relates to a microbiological composite bacterial inoculum and a preparation method thereof, in particular to the microbiological composite bacterial inoculum used for preparing a biological bacterial fertilizer. The microbiological composite bacterial inoculum is prepared by culturing Rhodop seudanonas palustris, Candida utilis, Lactobacillus acidophilus, Bacillus subtilis and Streptomyces microflavus var. lactosus which are disclosed in a culture catalogue of the China Center for General Microbiology Culture Collection (CGMCC), wherein the serial number of the Rhodop seudanonas palustris is 1.2352; the serial number of the Candida utilis is 2.1180; the serial number of the Lactobacillus acidophilus is 1.1854; the serial number of the Bacillus subtilis is 1.1630; the serial number of the Streptomyces microflavus var. lactosus is 4.0891; and the weight ratio of the Rhodop seudanonas palustris to the Candida utilis to the Lactobacillus acidophilus to the Bacillus subtilis is 1:0.5:0.5:0.1. When used for preparing the biological bacterial fertilizer, the composite bacterial inoculum can effectively decompose and release crude fibers and other nutrient components in straws and livestock manure; and the prepared biological organic fertilizer can condition the soil, increase the activity rate of microorganisms in the soil, avoid soil hardening, improve the permeability of the soil, play a role in production increasing, and effectively solve the problem of the area source pollution of agricultural wastes in rural areas.
Description
technical field [0001] The invention relates to a microbial composite bacterial agent and a preparation method thereof, in particular to a microbial composite bacterial agent for preparing biological bacterial fertilizer. Background technique [0002] The emergence of bio-organic fertilizers uses beneficial microorganisms in the soil to improve the micro-ecological system of crops and soil, releases the rich insoluble phosphorus and potassium in the soil, or converts difficult-to-use substances such as crop straws into usable The effective nutrients used can not only alleviate the contradiction between fertilizer supply and demand, but also improve the situation of ecological environment deterioration, and provide the possibility for the development of green ecological agriculture. It started. Biological fertilizers emerged in my country in the late 1980s. However, due to the fact that most of them are single bacteria and have single functions, they cannot fully solve the prob...
Claims
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Application Information
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