The invention relates to the field of digital image measurement, and proposes a plant three-dimensional measurement method and system based on PTZ camera system parameters and video frames. The method comprises the steps: S1, the calibration of PTZ camera system parameters: an axial image distance, image space and object space corresponding coefficients, an image shift rate pitching value fitting equation and coefficients; S2, a corresponding image point rectification model: an inter-frame image vector conversion model, an optical axis movement vector, a corresponding image point two-dimensional rectification matrix, and differential optimization verification; S3, point cloud world coordinate algorithm: a corresponding image point three-dimensional matrix, an inter-frame double-optical-system vector projection relation model, a PTZ camera system movement vector measurement model, three-dimensional point cloud coordinate obtaining, and a gray scale material correction factor; S4, plant local measurement method. The system comprises the following parts: S1, a PTZ camera system parameter obtaining module; S2, a corresponding image point rectification module; S3, a three-dimensional measurement module; S4, a plant local measurement module. Compared with other digital mapping technologies, the method does not need an object space control point, is strong in environmental adaptation, is high in equipment compatibility, and reduces the cost.