PRP preparation device and PRP preparation method

Abstract

The invention relates to a PRP preparation device and a PRP preparation method, the preparation device comprises a pipe body, an upper cover, a lower cover and a sealing plug, the pipe body comprises an upper section pipe, a middle pipe and a lower section pipe, the upper cover and the upper section pipe are matched to form an upper cavity, the upper cover can move up and down to enable the upper cover to be switched between a first position and a second position, and the upper cover is provided with a liquid injection and suction hole, a liquid suction pipe and a sealing piece; the lower cover is matched with the lower section pipe to form a lower cavity, and the lower cover can move up and down to adjust the volume of the lower cavity; the sealing plug is used for controlling opening and closing of the liquid injection and suction hole and the liquid suction pipe; the part, located in the upper cavity, of the pipette has a set length, and the set length enables the pipette to be located in the platelet-poor plasma layer and keep a gap with the platelet-rich plasma layer when the pipette is located at the second position, and enables the port of the pipette to be located outside the platelet-rich plasma when the port of the pipette is located at the first position. By applying the device, the PRP concentration can be directly adjusted in the preparation device, and the damage to platelets can be avoided when the PRP is extracted.

Description

Technical Field

[0001] The present invention relates to the technical field of medical devices, and in particular to a PRP preparation device and a PRP preparation method. Background Art

[0002] With the development of medical technology, platelet rich plasma (PRP) treatment technology has emerged. Platelet rich plasma is a platelet concentrate obtained by centrifuging autologous whole blood, which contains a large amount of growth factors and proteins. Injecting platelet rich plasma of appropriate concentration into the part that needs treatment has the effects of rapid hemostasis, analgesia, and accelerated wound healing, and has great value in medical beauty, wound treatment, and osteoarthritis treatment. At present, a platelet rich plasma preparation device is generally used to process the extracted autologous whole blood to obtain platelet rich plasma. It mainly centrifuges the preparation device containing autologous whole blood, so that the autologous whole blood is divided from top to bottom into a platelet poor plasma layer, a platelet rich plasma layer (generally referred to as a white film layer), and a red blood cell layer. Then, a certain amount of platelet poor plasma (PPP) and platelet rich plasma are respectively absorbed using accessories such as a Luer connector and a syringe, and then the two are mixed and shaken in an external container to obtain the required concentration of platelet rich plasma.

[0003] However, since PPP and PRP need to be sucked out of the preparation device separately and then shaken and mixed, such PRP preparation devices and preparation methods have a high risk of infection in application. In addition, the current PRP preparation devices and preparation methods also have problems such as a small scope of application and easy damage to platelets. Summary of the invention

[0004] Based on this, it is necessary to provide a PRP preparation device and a PRP preparation method to address the above technical problems.

[0005] The present invention provides a PRP preparation device, comprising:

[0006] A pipe body, comprising an upper pipe section, a middle pipe section and a lower pipe section which are connected in sequence, wherein the inner diameters of the upper pipe section and the lower pipe section are both larger than the inner diameter of the middle pipe section;

[0007] An upper cover is arranged on the upper tube and the two cooperate to form an upper cavity, the upper cover is provided with a liquid injection and suction hole, a liquid suction tube and a sealing member, the liquid injection and suction hole and the liquid suction tube both connect the upper cavity with the outside, and the sealing member and the liquid suction tube both extend into the upper cavity;

[0008] A lower cover, which is disposed on the lower tube and the two cooperate to form a lower cavity, and the lower cover can be displaced up and down relative to the lower tube to adjust the volume of the lower cavity; and

[0009] A sealing plug, which is arranged on the upper cover and is used to control the opening and closing of the injection and suction holes and the suction tube;

[0010] The upper cover can be displaced up and down relative to the upper tube so that the upper cover can be switched between a first position and a second position. When the upper cover is in the first position, the sealing member is separated from the middle tube, and when the upper cover is in the second position, the middle tube is sealed to isolate the upper cavity from the lower cavity.

[0011] The portion of the pipette located in the upper cavity has a set length, and the set length is configured to enable the port of the pipette to be located in the platelet-poor plasma layer when the upper cover is in the first position and the tube body is upright, to enable the port of the pipette to maintain a distance from the platelet-rich plasma layer when the upper cover is in the second position and the tube body is upright, and to enable the port of the pipette to be located outside the shaken platelet-rich plasma when the upper cover is in the second position and the tube body is inverted.

[0012] The application of this application has the following beneficial effects:

[0013] 1. The volume of the lower cavity can be adjusted by moving the lower cover up and down relative to the lower tube, and then the volume of the preparation device can be adjusted. The volume of the preparation device can be adjusted accordingly according to the target blood volume required by the treatment plan, which can expand the application scope of the preparation device and reduce unnecessary blood waste;

[0014] 2. After centrifugation and stratification, the volume of the lower chamber can be changed by shifting the lower cover, so that the platelet-rich plasma layer can rise to the upper chamber, and then the middle tube can be sealed by the seal, so that the platelet-poor plasma layer and the platelet-rich plasma layer can be completely separated from the red blood cell layer, and then the platelet-poor plasma can be extracted through the pipette according to the required concentration to adjust the platelet-poor plasma and platelet-rich plasma to a suitable ratio. After the two are mixed and shaken, the platelet-rich plasma of the required concentration can be obtained, avoiding mixing operations outside the preparation device and reducing the risk of infection;

[0015] 3. The preparation device can be inverted to extract platelet-rich plasma. Since the port of the pipette is located outside the shaken platelet-rich plasma, the pipette connected to the outside can balance the air pressure. A syringe without a needle can be used to extract the shaken platelet-rich plasma through the injection and aspiration holes to avoid damaging platelets and contamination caused by needle insertion. At the same time, the inverted preparation device is easier to completely extract platelet-rich plasma to avoid residual waste.

[0016] In one embodiment, the set length is L, the height dimension of the upper cavity when the upper cover is in the second position is H, and L and H satisfy the following relationship:

[0017] In one embodiment, the upper cover is threadedly connected to the upper tube, and the upper cover is displaced up and down relative to the upper tube by screwing the thread. The upper cover is displaced up and down relative to the upper tube by screwing. On the one hand, the screwing operation along the circumference is not easy to cause vibration of the tube body, avoiding the influence on the plasma layered after centrifugation; on the other hand, the threaded connection is easy to meet the sealing requirements.

[0018] In one embodiment, the upper cover includes an upper cover plate and an upper screw cap which are formed into an integral structure, the injection and suction holes, the suction tube and the sealing member are all arranged on the upper cover plate, the upper screw cap is provided with an internal thread, and the upper cover is screwed onto the outside of the upper tube through the upper screw cap. The upper cover is screwed onto the outside of the upper tube through the upper screw cap to facilitate the screwing operation.

[0019] In one embodiment, the lower cover is threadedly connected to the lower tube, and the lower cover is displaced up and down relative to the lower tube by screwing. The lower cover is displaced up and down relative to the lower tube by screwing. On the one hand, the screwing operation along the circumferential direction is not easy to cause vibration of the tube body, avoiding the influence on the plasma layered after centrifugation; on the other hand, the threaded connection is easy to meet the sealing requirements.

[0020] In one embodiment, the lower cover includes a lower cover plate and a lower screw cap formed as an integral structure, the lower screw cap is provided with an internal thread, and the lower cover is screwed onto the outside of the lower tube section through the lower screw cap. The lower cover is screwed onto the outside of the lower tube section through the lower screw cap to facilitate the screwing operation.

[0021] In one of the embodiments, the lower cover further comprises a push block fixedly disposed in the lower screw cap, the push block is slidably disposed inside the lower tube section, and the outer surface of the push block is in contact with the inner wall of the lower tube section.

[0022] In one embodiment, the upper tube is provided with scale lines, and the scale lines are used to indicate the volume value of the space below the position of the corresponding scale lines in the upper cavity. The scale values ​​are set to facilitate observation of the amount of platelet-rich plasma and platelet-poor plasma, and to facilitate determination of the amount of platelet-poor plasma that needs to be drawn out.

[0023] In one embodiment, the tube body is made of a transparent material, and a convex structure is formed inside the middle tube. By forming the convex structure inside the middle tube, a visual magnification effect can be formed through the convex structure, so that the operator can observe the concentration state of the platelets from the outside of the middle tube.

[0024] In one embodiment, the intermediate tube forms an intermediate cavity, the upper cavity, the intermediate cavity and the lower cavity are coaxially distributed, and the sealing member is a sealing shaft extending downward through the middle of the upper cover; the upper cavity includes an upper main cavity and an upper transition cavity located below the upper main cavity, and the upper transition cavity gradually decreases from top to bottom in width; the lower cavity includes a lower main cavity and a lower transition cavity located above the lower main cavity, and the lower transition cavity gradually increases from top to bottom in width, and the upper transition cavity, the intermediate cavity and the lower transition cavity are connected in sequence. In this way, when the upper cover is screwed, the sealing member rotates around its own axis while following the upper cover's up and down displacement, which can reduce disturbance to the platelet-rich plasma.

[0025] In one embodiment, the sealing member is detachably connected to the upper cover or formed as an integral structure;

[0026] The sealing member is inserted into the middle cavity to seal the middle tube when the upper cover is in the second position;

[0027] Alternatively, the inner diameter of the bottom of the upper transition chamber is greater than the inner diameter of the middle chamber and a step surface is formed therebetween, and the sealing member abuts against the step surface to close the middle tube when the upper cover is at the second position.

[0028] In addition, the present application also provides a PRP preparation method, which is implemented by using the PRP preparation device as described in the above technical solution, and the preparation method comprises the following steps:

[0029] In a state where the sealing member and the intermediate tube remain separated, opening the sealing plug and injecting the blood to be treated into the tube body through the injection and suction holes;

[0030] Close the sealing plug and place the preparation device in a centrifuge for centrifugation, so that the blood in the tube is separated from top to bottom into a platelet-poor plasma layer, a platelet-rich plasma layer and a red blood cell layer;

[0031] adjusting the position of the lower cover relative to the tube body to push the platelet-rich plasma layer into the upper chamber;

[0032] Aspirating the platelet-poor plasma layer through the pipette until the platelet-poor plasma and platelet-rich plasma reach a target ratio;

[0033] Adjusting the upper cover to a second position relative to the tube body so that the sealing member seals the middle tube;

[0034] Shake the platelet-poor plasma layer and the platelet-rich plasma layer in the upper chamber and invert the preparation device;

[0035] Open the sealing stopper and draw platelet-rich plasma through the injection and aspiration holes.

[0036] The preparation method provided in this application is similar to the reasoning process of the beneficial effects of the aforementioned preparation device, and will not be repeated here.

[0037] In one embodiment, before injecting the blood to be processed into the tube body through the injection and suction hole, the following steps are also included: adjusting the position of the lower cover relative to the tube body to adjust the internal volume of the tube body to match the amount of blood to be processed;

[0038] And / or, after injecting the blood to be processed into the tube body through the injection and suction hole, the method further includes the following steps: adjusting the position of the lower cover relative to the tube body to adjust the volume of the lower cavity. BRIEF DESCRIPTION OF THE DRAWINGS

[0039] Figure 1 A schematic diagram of the external structure of the preparation device provided in this application;

[0040] Figure 2 This is an exploded view of the preparation device;

[0041] Figure 3 A longitudinal cross-sectional view of the preparation device when the upper cover is in the first position and the lower cover is in a relatively lower position;

[0042] Figure 4 A longitudinal cross-sectional view of the preparation device when the upper cover is in the first position and the lower cover is in a relatively upper position;

[0043] Figure 5 is a longitudinal cross-sectional view of the preparation device when the upper cover is in the second position and the lower cover is in a relatively upper position;

[0044] Figure 6 It is a longitudinal cross-sectional view of the preparation device when it is inverted to extract platelet-rich plasma;

[0045] Figure 7 The present invention provides a flow chart of the preparation method.

[0046] Figure numerals: 1. upper tube; 2. middle tube; 20. step surface; 21. convex structure; 3. lower tube; 4. upper cover; 40. upper cover plate; 400. injection and suction holes; 41. upper screw cap; 42. suction tube; 43. sealing member; 44. sealing ring; 5. lower cover; 50. lower cover plate; 51. lower screw cap; 52. push block; 6. sealing plug; 7. upper cavity; 70. upper main cavity; 71. upper transition cavity; 8. middle cavity; 9. lower cavity; 90. lower main cavity; 91. lower transition cavity. DETAILED DESCRIPTION

[0047] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the specific embodiments of the present invention are described in detail below in conjunction with the accompanying drawings. In the following description, many specific details are set forth to facilitate a full understanding of the present invention. However, the present invention can be implemented in many other ways different from those described herein, and those skilled in the art can make similar improvements without violating the connotation of the present invention, so the present invention is not limited by the specific embodiments disclosed below.

[0048] In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise", "axial", "radial", "circumferential" and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as limiting the present invention.

[0049] In addition, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include at least one of the features. In the description of the present invention, the meaning of "plurality" is at least two, such as two, three, etc., unless otherwise clearly and specifically defined.

[0050] In the present invention, unless otherwise clearly specified and limited, the terms "installed", "connected", "connected", "fixed" and the like should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements, unless otherwise clearly defined. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0051] In the present invention, unless otherwise clearly specified and limited, a first feature being "above" or "below" a second feature may mean that the first and second features are in direct contact, or the first and second features are in indirect contact through an intermediate medium. Moreover, a first feature being "above", "above" or "above" a second feature may mean that the first feature is directly above or obliquely above the second feature, or simply means that the first feature is higher in level than the second feature. A first feature being "below", "below" or "below" a second feature may mean that the first feature is directly below or obliquely below the second feature, or simply means that the first feature is lower in level than the second feature.

[0052] It should be noted that when an element is referred to as being "fixed to" or "disposed on" another element, it may be directly on the other element or there may be a central element. When an element is considered to be "connected to" another element, it may be directly connected to the other element or there may be a central element at the same time. The terms "vertical", "horizontal", "upper", "lower", "left", "right" and similar expressions used herein are for illustrative purposes only and are not intended to be the only implementation method.

[0053] The PRP concentration required in different treatment plans is different. For example, in medical aesthetics, the PRP concentration multiple is preferably 2-3 times; in wound treatment, the PRP concentration multiple is preferably controlled at about 4 times; in osteoarthritis treatment, the PRP concentration multiple is preferably about 6 times. After the existing PRP preparation device is centrifuged, in order to obtain the required concentration of platelet-rich plasma, it is necessary to use accessories such as Luer connectors and syringes to absorb a certain amount of platelet-poor plasma and platelet-rich plasma respectively, and then mix the two in an external container and shake them to obtain the required concentration of platelet-rich plasma. The operation of aspirating platelet-poor plasma and platelet-rich plasma into an external container for mixing increases the probability of infection.

[0054] In the process of drawing platelet-poor plasma and platelet-rich plasma, since there is only one opening for the syringe to draw, under the combined effect of atmospheric pressure and liquid molecule surface tension, it is difficult to draw out the platelet-poor plasma and platelet-rich plasma by the syringe alone, and a needle needs to be added to the syringe to complete the drawing, and the needle will damage the platelets in the platelet-rich plasma. Moreover, the use of this drawing scheme will inevitably cause some platelet-poor plasma and platelet-rich plasma to remain in the preparation device.

[0055] In addition, the amount of platelet-rich plasma required in different treatment plans is also different, and correspondingly, the amount of autologous whole blood sample required is also different. However, the preparation device in the existing PPP preparation device can only process a fixed amount of autologous whole blood, and the application range is relatively small.

[0056] To solve the above problems, the first aspect of the present application provides a PRP preparation device, such as Figure 1 and Figure 2 As shown in the figure, the preparation device includes a tube body, an upper cover 4, a lower cover 5 and a sealing plug 6. The upper cover 4 is provided with a liquid injection and aspiration hole 400, a liquid aspiration tube 42 and a sealing member 43. The tube body includes an upper tube 1, an intermediate tube 2 and a lower tube 3 which are connected in sequence. When autologous whole blood is injected into the tube body, it can flow in the upper tube 1, the intermediate tube 2 and the lower tube 3. The inner diameters of the upper tube 1 and the lower tube 3 are both larger than the inner diameter of the intermediate tube 2. Figure 3 and Figure 4 As shown in the figure, the upper cover 4 is arranged on the upper section tube 1 and the two cooperate to form an upper cavity 7, the injection and suction holes 400 and the suction tube 42 both connect the upper cavity 7 with the outside, and the sealing plug 6 is arranged on the upper cover 4 and is used to control the opening and closing of the injection and suction holes 400 and the suction tube 42.

[0057] Combination Figure 4 and Figure 5 As shown, the lower cover 5 is arranged on the lower tube 3 and the two cooperate to form the lower cavity 9. The lower cover 5 can be displaced up and down relative to the lower tube 3 to adjust the volume of the lower cavity 9. The lower cover 5 can be displaced up and down relative to the lower tube 3 to adjust the volume of the lower cavity 9, and then the volume of the preparation device can be adjusted, so that the volume of the preparation device can be adjusted accordingly according to the target blood volume required by the treatment plan, which can expand the application scope of the preparation device and reduce unnecessary blood waste. At the same time, the upper cover 4 can be displaced up and down relative to the upper tube 1 to switch the upper cover 4 between the first position and the second position, and the sealing member 43 and the pipette 42 are both extended into the upper cavity 7. The sealing member 43 is separated from the middle tube 2 when the upper cover 4 is in the first position, and the middle tube 2 is sealed when the upper cover 4 is in the second position to isolate the upper cavity 7 from the lower cavity 9.

[0058] The portion of the pipette 42 in the present embodiment located in the upper chamber 7 has a set length, and the set length is configured to enable the port of the pipette 42 to be located in the platelet-poor plasma layer when the upper cover 4 is in the first position and the tube body is placed upright. After centrifugal stratification, the platelet-rich plasma layer can be raised to the upper chamber 7 by shifting the lower cover 5 to change the volume of the lower chamber 9, and then the intermediate tube 2 is closed by the seal 43, so that the platelet-poor plasma layer and the platelet-rich plasma layer can be completely isolated from the red blood cell layer. After observing and determining the amount of platelet-rich plasma obtained after centrifugal stratification, the required amount of platelet-poor plasma can be deduced according to the required concentration of platelet-rich plasma, and the platelet-poor plasma is sucked by the pipette 42, so that the platelet-poor plasma and platelet-rich plasma in the upper chamber 7 reach the required ratio, and the two are mixed and shaken to obtain the platelet-rich plasma of the required concentration. Avoid mixing operations outside the preparation device to reduce the risk of infection.

[0059] It is easy to understand that the pipette 42 is used to absorb platelet-poor plasma, that is, the pipette 42 cannot extend into the platelet-rich plasma layer after stratification. Therefore, the set length is also configured to enable the port of the pipette 42 to maintain a distance from the platelet-rich plasma layer when the upper cover 4 is in the second position and the tube body is placed upright.

[0060] In addition, if Figure 6As shown in , the set length is also configured to enable the port of the pipette 42 to be located outside the shaken platelet-rich plasma when the upper cover 4 is in the second position and the tube body is inverted. In this way, after the platelet-poor plasma and the platelet-rich plasma are mixed and shaken, the preparation device can be inverted to extract the platelet-rich plasma. Since the port of the pipette 42 is located outside the shaken platelet-rich plasma, the pipette 42 connected to the outside can balance the air pressure, and a syringe without a needle can be used to extract the shaken platelet-rich plasma through the injection and suction hole 400, which can avoid damaging platelets and avoid contamination caused by needle insertion. At the same time, the inverted preparation device is easier to completely extract the platelet-rich plasma to avoid residual waste.

[0061] The "upright placement" described in this embodiment refers to placing the preparation device in a manner where "the upper cover 4 is located on the upper side and the lower cover 5 is located on the lower side"; the "inverted placement" described in this embodiment refers to placing the preparation device in a manner where "the upper cover 4 is located on the lower side and the lower cover 5 is located on the upper side".

[0062] Specifically in this embodiment, Figure 5 As shown in , the set length is L, the height dimension of the upper cavity 7 when the upper cover 4 is in the second position is H, and L and H satisfy the following relationship: In other optional embodiments, L and H satisfy the following relationship: That's it. The above relationship is obtained through deduction and experiment, and its principle is explained as follows: As mentioned above, after the required amount of platelet-rich plasma of the required concentration is clear, the corresponding amount of autologous whole blood sample has been roughly determined, and the corresponding tube volume is also roughly determined. In this case, after centrifugal stratification and allowing the platelet-rich plasma layer to enter the upper chamber 7, the height position of the platelet-rich plasma layer in the upper tube 1 and the height position of the platelet-poor plasma layer in the upper tube 1 can be deduced to obtain similar data. After the preparation device is inverted, the height position of the mixed and shaken platelet-rich plasma of the required concentration in the upper tube 1 can also be deduced to obtain similar data. Through multiple experiments, the relationship between the above-mentioned set length L and the height dimension H of the upper chamber 7 when the upper cover 4 is in the second position can be obtained.

[0063] The upper cover 4 in this embodiment is threadedly connected to the upper tube 1, and the upper cover 4 is displaced up and down relative to the upper tube 1 through the threaded screwing action. The upper cover 4 is displaced up and down relative to the upper tube 1 by the screwing operation. On the one hand, the screwing operation along the circumferential direction is not easy to cause vibration of the tube body, avoiding the influence on the stratified plasma after centrifugation; on the other hand, the threaded connection is easy to meet the sealing requirements. Specifically, the upper cover 4 in this embodiment includes an upper cover plate 40 and an upper screw cap 41 formed as an integral structure, the injection and suction holes 400, the suction tube 42 and the sealing member 43 are all arranged on the upper cover plate 40, and the upper screw cap 41 is provided with an internal thread. The upper cover 4 is screwed on the outside of the upper tube 1 through the upper screw cap 41. The upper cover 4 is screwed on the outside of the upper tube 1 through the upper screw cap 41 to facilitate the screwing operation.

[0064] To achieve threaded connection, the upper tube 1 in this embodiment is provided with an external thread on its outer wall that matches the internal thread of the upper screw cap 41. In other optional embodiments, the upper cover 4 can also be designed as a columnar structure, with an external thread on the outside of the columnar structure, and an internal thread matching the external thread of the above structure on the inner wall of the upper tube 1. In this case, since the columnar structure has a larger dimension in the height direction, the depth dimension of the injection and suction hole 400 is larger.

[0065] Similar to the structural design of the upper cover 4, the lower cover 5 in this embodiment is threadedly connected to the lower tube 3, and the lower cover 5 is displaced up and down relative to the lower tube 3 through the threaded screwing action. The lower cover 5 is displaced up and down relative to the lower tube 3 by the screwing operation. On the one hand, the screwing operation along the circumferential direction is not easy to cause vibration of the tube body, avoiding the influence on the stratified plasma after centrifugation; on the other hand, the threaded connection is easy to meet the sealing requirements. Specifically, the lower cover 5 in this embodiment includes a lower cover plate 50 and a lower screw cap 51 formed as an integral structure, the lower screw cap 51 is provided with an internal thread, and the outer part of the lower tube 3 is provided with an external thread, and the lower cover 5 is screwed to the outer part of the lower tube 3 through the lower screw cap 51. The lower cover 5 is screwed to the outer part of the lower tube 3 through the lower screw cap 51 to facilitate the screwing operation.

[0066] Furthermore, the lower cover 5 in this embodiment also includes a push block 52 fixedly disposed in the lower screw cap 51, the push block 52 can be slidably disposed inside the lower tube 3, and the outer surface of the push block 52 fits with the inner wall of the lower tube 3. Optionally, the push block 52 can be made of a material with a certain elastic deformation ability, for example, the push block 52 can be a rubber block, so that it can fit closely with the inner wall of the lower tube 3, and play a good sealing role for the plasma. The push block 52 in this embodiment is fixedly connected to the lower cover plate 50, and optionally, the push block 52 and the lower cover plate 50 can also be integrally formed and manufactured.

[0067] Since the lower chamber 9 is formed by the cooperation of the lower tube 3 and the lower cover 5, in the embodiment without the push block 52, the volume of the lower chamber 9 can also be changed by the up and down displacement of the lower cover plate 50 and the lower screw cap 51. The lower cover 5 in this embodiment adopts the scheme of setting the push block 52, which is more convenient for the overall size design of the tube body, thereby making the preparation device as a whole easier to operate and hold. The specific description is as follows: when the lower cover 5 adopts the scheme of not setting the push block 52, the lower chamber 9 is formed by the cooperation of the lower cover plate 50 and the lower knob cap in the lower cover 5 and the lower tube 3; when the lower cover 5 adopts the scheme of setting the push block 52, the lower chamber 9 is formed by the cooperation of the push block 52 in the lower cover 5 and the lower tube 3. After the maximum design volume and the minimum design volume of the lower chamber 9 are set, the length dimension of the lower tube 3 in the scheme of setting the push block 52 will be designed to be longer, which is easier to operate and hold.

[0068] In this embodiment, scale lines are provided on the upper tube 1, and the scale lines are used to represent the volume value of the space below the position of the corresponding scale lines in the upper cavity 7. The scale values ​​are set to facilitate observation of the amount of platelet-rich plasma and platelet-poor plasma, and to facilitate determination of the amount of platelet-poor plasma that needs to be drawn out.

[0069] The tube body in this embodiment is made of transparent material, and a convex structure 21 is formed inside the middle tube 2. By forming the convex structure 21 inside the middle tube 2, a visual magnification effect can be formed through the convex structure 21, which is convenient for the operator to observe the concentrated state of platelets from the outside of the middle tube 2. The tube body in this embodiment is made of glass material. In other optional embodiments, the tube body can also be made of transparent plastic materials such as polypropylene. The convex structure 21 refers to the wall thickness of the middle tube 2 having the characteristics of "relatively thick in the middle and relatively thin on the upper and lower sides" along the height direction of the tube body, so that the middle tube 2 forms a structure similar to a "magnifying glass", thereby having a visual magnification effect.

[0070] The middle tube 2 in this embodiment is formed with a middle cavity 8, the upper cavity 7, the middle cavity 8 and the lower cavity 9 are coaxially distributed, and the sealing member 43 is a sealing shaft extending downward through the middle of the upper cover 4. In this way, when the upper cover 4 is screwed, the sealing member 43 rotates around its own axis while following the upper cover 4 to move up and down, thereby reducing the disturbance to the platelet-rich plasma.

[0071] Furthermore, the upper chamber 7 in this embodiment includes an upper main chamber 70 and an upper transition chamber 71 located below the upper main chamber 70, and the upper transition chamber 71 gradually decreases in width from top to bottom; the lower chamber 9 includes a lower main chamber 90 and a lower transition chamber 91 located above the lower main chamber 90, and the lower transition chamber 91 gradually increases in width from top to bottom, and the upper transition chamber 71, the middle chamber 8 and the lower transition chamber 91 are connected in sequence. The above-mentioned configuration of the tube body facilitates the sealing operation of the sealing member 43 and the middle chamber 8 on the one hand; on the other hand, the inner walls of the upper transition chamber 71 and the lower transition chamber 91 are designed with inclined surfaces, so that the platelet-rich plasma layer after centrifugal stratification is not easily dispersed during the upward displacement to the upper chamber 7.

[0072] like Figures 1 to 5 As shown in , the inner diameters of the upper main body cavity 70 and the lower main body cavity 90 in this embodiment are the same, the upper transition cavity 71 and the lower transition cavity 91 are roughly symmetrical with respect to the middle cavity 8, and the wall thickness of the upper tube 1 is roughly the same as the wall thickness of the lower tube 3. Therefore, the wall of the upper tube 1 is substantially the same as the contour of the upper cavity 7, and the wall of the lower tube 3 is substantially the same as the contour of the lower cavity 9. It should be noted that in other optional embodiments, the outer contour of the tube body can be directly designed as a columnar shape, and it is only necessary to ensure that the upper cavity 7, the middle cavity 8 and the lower cavity 9 meet the aforementioned characteristics, and the wall thickness of the tube body can be changed.

[0073] like Figure 2 As shown in , the seal 43 in this embodiment is detachably connected to the upper cover 4, the seal 43 is arranged through the upper cover plate 40, and a sealing ring 44 can be arranged between the seal 43 and the upper cover plate 40 for sealing. In other optional embodiments, the seal 43 can also be integrally formed with the upper cover 4.

[0074] like Figure 5 As shown in FIG. 1 , in this embodiment, the sealing member 43 is designed to be compatible with the middle cavity 8. When the upper cover 4 is in the second position, the sealing member 43 is inserted into the middle cavity 8 to seal the middle tube 2. Figure 3 As shown in the figure, the bottom inner diameter of the upper transition chamber 71 is larger than the inner diameter of the middle chamber 8 and a step surface 20 is formed therebetween. In other optional embodiments, the diameter of the seal 43 can also be designed to be larger than the inner diameter of the middle chamber 8, so that the seal 43 can abut against the step surface 20 to close the middle tube 2 when the upper cover 4 is in the second position.

[0075] The second aspect of the present application provides a method for preparing PRP, which is implemented using the above-mentioned preparation device, such as Figure 7 As shown in, the preparation method comprises the following steps:

[0076] S100: With the sealing member 43 and the intermediate tube 2 kept separated, the sealing plug 6 is opened and the blood to be treated is injected into the tube body through the injection and suction hole 400;

[0077] S200: Close the sealing plug 6 and place the preparation device in a centrifuge for centrifugation, so that the blood in the tube is separated from top to bottom into a platelet-poor plasma layer, a platelet-rich plasma layer and a red blood cell layer; a cross-sectional view of the preparation device after injecting autologous whole blood and closing the sealing plug 6 is shown in FIG. Figure 3 as shown in .

[0078] S300: Adjust the position of the lower cover 5 relative to the tube body to push the platelet-rich plasma layer into the upper chamber 7; the cross-sectional view of the preparation device after this step is completed is as follows Figure 4 as shown in .

[0079] S400: aspirating the platelet-poor plasma layer through the pipette 42 until the platelet-poor plasma and platelet-rich plasma reach a target ratio;

[0080] S500: Adjust the upper cover 4 to the second position relative to the tube body so that the seal 43 seals the intermediate tube 2; the cross-sectional view of the preparation device after this step is completed is as follows Figure 5 as shown in .

[0081] S600: Shake the platelet-poor plasma layer and the platelet-rich plasma layer in the upper chamber 7 and invert the preparation device; a cross-sectional view of the preparation device during this step is shown in FIG. Figure 6 It should be noted that in this step, the platelet-poor plasma layer and the platelet-rich plasma layer in the upper chamber 7 can be mixed and shaken first, and then the preparation device can be inverted; or the preparation device can be inverted first, and then the platelet-poor plasma layer and the platelet-rich plasma layer in the upper chamber 7 can be shaken. Among them, the preparation device can be inverted first and then the shaking operation can save time for waiting for the preparation device to stand.

[0082] S700: Open the sealing plug 6 and extract the shaken platelet-rich plasma through the injection and aspiration hole 400.

[0083] In other optional embodiments, before injecting the blood to be treated into the tube body through the injection and suction hole 400 in step S100, step S10 may also be included: adjusting the position of the lower cover 5 relative to the tube body to adjust the internal volume of the tube body to match the amount of blood to be treated. Step S10 can adjust the volume of the preparation device according to the target blood volume required by the treatment plan, which can expand the scope of application of the preparation device and reduce unnecessary blood waste.

[0084] In addition, after step S100, step S110 may be included: adjusting the position of the lower cover 5 relative to the tube body to adjust the volume of the lower cavity 9. That is, the position adjustment operation of the lower cover 5 may be performed after the autologous whole blood is injected into the tube body, which can also play the role of "adjusting the volume of the preparation device according to the target blood volume required by the treatment plan". At the same time, after the autologous whole blood is injected into the tube body, the lower cover 5 is moved downward and upward, which can change the volume of the lower cavity 9 so that the autologous whole blood can flow more fully in the tube body.

[0085] It is easy to understand that both step S10 and step S100 may be used in one operation, or only one of them may be used.

[0086] The technical features of the above-described embodiments may be arbitrarily combined. To make the description concise, not all possible combinations of the technical features in the above-described embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0087] The above-mentioned embodiments only express several implementation methods of the present invention, and the descriptions thereof are relatively specific and detailed, but they cannot be understood as limiting the scope of the invention patent. It should be pointed out that, for ordinary technicians in this field, several variations and improvements can be made without departing from the concept of the present invention, and these all belong to the protection scope of the present invention. Therefore, the protection scope of the patent of the present invention shall be subject to the attached claims.

Claims

1. A PRP preparation device, characterized in that: The preparation device comprises: A pipe body, comprising an upper pipe (1), a middle pipe (2) and a lower pipe (3) which are connected in sequence, wherein the inner diameters of the upper pipe (1) and the lower pipe (3) are both larger than the inner diameter of the middle pipe (2); An upper cover (4) is arranged on the upper tube (1) and the two cooperate to form an upper chamber (7); the upper cover (4) is provided with a liquid injection and suction hole (400), a liquid suction tube (42) and a sealing member (43); the liquid injection and suction hole (400) and the liquid suction tube (42) both connect the upper chamber (7) with the outside, and the sealing member (43) and the liquid suction tube (42) both extend into the upper chamber (7); A lower cover (5) is disposed on the lower tube (3) and the two cooperate to form a lower chamber (9), wherein the lower cover (5) can be displaced up and down relative to the lower tube (3) to adjust the volume of the lower chamber (9); and A sealing plug (6) is arranged on the upper cover (4) and is used to control the opening and closing of the liquid injection and suction holes (400) and the liquid suction tube (42); The upper cover (4) can be displaced up and down relative to the upper tube (1) so that the upper cover (4) can be switched between a first position and a second position; the sealing member (43) is separated from the middle tube (2) when the upper cover (4) is in the first position, and seals the middle tube (2) to isolate the upper chamber (7) from the lower chamber (9) when the upper cover (4) is in the second position; The portion of the pipette (42) located in the upper chamber (7) has a set length, and the set length is configured so that when the upper cover (4) is in the first position and the tube body is placed upright, the port of the pipette (42) can be located in the platelet-poor plasma layer, when the upper cover (4) is in the second position and the tube body is placed upright, the port of the pipette (42) can be kept apart from the platelet-rich plasma layer, and when the upper cover (4) is in the second position and the tube body is inverted, the port of the pipette (42) can be located outside the platelet-rich plasma in a shaken state.

2. The preparation device according to claim 1, characterized in that: The set length is L, the height dimension of the upper cavity (7) when the upper cover (4) is in the second position is H, and L and H satisfy the following relationship:

3. The preparation device according to claim 1, characterized in that: The upper cover (4) is threadedly connected to the upper tube (1), and the upper cover (4) is displaced up and down relative to the upper tube (1) through a screwing action of the thread.

4. The preparation device according to claim 3, characterized in that: The upper cover (4) comprises an upper cover plate (40) and an upper screw cap (41) which are formed into an integral structure; the liquid injection and suction holes (400), the liquid suction tube (42) and the sealing member (43) are all arranged on the upper cover plate (40); the upper screw cap (41) is provided with an internal thread; and the upper cover (4) is screwed onto the outside of the upper tube (1) via the upper screw cap (41).

5. The preparation device according to claim 1, characterized in that: The lower cover (5) is threadedly connected to the lower tube (3), and the lower cover (5) is displaced up and down relative to the lower tube (3) by a screwing action of the thread.

6. The preparation device according to claim 5, characterized in that The lower cover (5) comprises a lower cover plate (50) and a lower screw cap (51) which are formed into an integral structure. The lower screw cap (51) is provided with an internal thread. The lower cover (5) is screwed onto the outside of the lower section pipe (3) through the lower screw cap (51).

7. The preparation device according to claim 6, characterized in that: The lower cover (5) further comprises a push block (52) fixedly arranged in the lower screw cap (51); the push block (52) is slidably arranged inside the lower tube section (3); and the outer surface of the push block (52) is in contact with the inner wall of the lower tube section (3).

8. The preparation device according to claim 1, characterized in that: The upper tube (1) is provided with scale lines, and the scale lines are used to represent the volume value of the space below the position of the corresponding scale lines in the upper chamber (7).

9. The preparation device according to claim 1, characterized in that: The tube body is made of a transparent material, and a convex structure (21) is formed inside the middle tube (2).

10. The preparation device according to any one of claims 1 to 9, characterized in that: The intermediate tube (2) is formed with an intermediate cavity (8), the upper cavity (7), the intermediate cavity (8) and the lower cavity (9) are coaxially distributed, and the sealing member (43) is a sealing shaft extending downward through the middle of the upper cover (4); The upper chamber (7) comprises an upper main chamber (70) and an upper transition chamber (71) located below the upper main chamber (70), wherein the width of the upper transition chamber (71) gradually decreases from top to bottom; the lower chamber (9) comprises a lower main chamber (90) and a lower transition chamber (91) located above the lower main chamber (90), wherein the width of the lower transition chamber (91) gradually increases from top to bottom, and the upper transition chamber (71), the middle chamber (8) and the lower transition chamber (91) are connected in sequence.

11. The preparation device according to claim 10, characterized in that: The sealing member (43) and the upper cover (4) are detachably connected or form an integral structure; The sealing member (43) is inserted into the middle cavity (8) to seal the middle tube (2) when the upper cover (4) is in the second position; Alternatively, the inner diameter of the bottom of the upper transition chamber (71) is greater than the inner diameter of the middle chamber (8) and a step surface (20) is formed between the two, and the sealing member (43) abuts against the step surface (20) to seal the middle tube (2) when the upper cover (4) is in the second position.

12. A method for preparing PRP, characterized in that: The preparation method is implemented by using a preparation device as described in any one of claims 1 to 11, and the preparation method comprises the following steps: While the sealing member (43) and the intermediate tube (2) remain separated, the sealing plug (6) is opened and the blood to be treated is injected into the tube body through the injection and suction hole (400); Close the sealing plug (6) and place the preparation device in a centrifuge for centrifugation, so that the blood in the tube is separated from top to bottom into a platelet-poor plasma layer, a platelet-rich plasma layer and a red blood cell layer; Adjusting the position of the lower cover (5) relative to the tube body to push the platelet-rich plasma layer into the upper chamber (7); Aspirating the platelet-poor plasma layer through the pipette (42) until the platelet-poor plasma and platelet-rich plasma reach a target ratio; The upper cover (4) is adjusted to a second position relative to the tube body so that the sealing member (43) seals the middle tube (2); Shake the platelet-poor plasma layer and the platelet-rich plasma layer in the upper chamber (7) and invert the preparation device; The sealing plug (6) is opened to extract platelet-rich plasma through the injection and aspiration hole (400).

13. The preparation method according to claim 12, characterized in that: Before injecting the blood to be treated into the tube body through the injection and suction hole (400), the following steps are also included: adjusting the position of the lower cover (5) relative to the tube body to adjust the internal volume of the tube body to match the amount of blood to be treated; And / or, after injecting the blood to be treated into the tube body through the injection and suction hole (400), the method further comprises the following steps: adjusting the position of the lower cover (5) relative to the tube body to adjust the volume of the lower chamber (9).

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