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53results about How to "Good quality extract" patented technology

All-weather unmanned autonomous working platform in unknown environment

The invention discloses an unmanned autonomous working platform based on an all-weather unknown environment, and belongs to the field of artificial intelligence and visual navigation. The platform comprises five modules: a stereoscopic vision positioning module, an infrared visible light fusion module, an image recognition module, a map construction module and a loop-back and return detection module. The visual positioning module and the image recognition module share a graph convolutional neural network framework, the visual positioning module selects key frames to perform feature matching and visual positioning, the image recognition module performs semantic classification on a point cloud local map, and the map construction module performs point cloud splicing to form a global depth dense semantic map. The deep neural network is introduced to improve the feature extraction effect and save the extraction time. Monocular vision distance measurement is adopted, so that the multi-parallax registration time is saved. Multi-spectral fusion of key frame images is carried out, all-weather efficient work is achieved, and the detection rate of shielded targets is increased.
Owner:JILIN UNIV

Instantaneous elasticity imaging code detection method and system

ActiveCN105844645AImprove the problem of insufficient depth penetrationIncreased average launch energyImage enhancementImage analysisSonificationSignal-to-quantization-noise ratio
The present invention discloses an instantaneous elasticity imaging code detection method and a system. The method comprises the steps: an instantaneous low-frequency signal sent by a signal generator is subjected to power amplification to drive an electromagnetic vibrator and motivate the generation of an instantaneous shear wave inside a medium; modulating the coding of an ultrasonic pulse signal, detecting and tracking the vibration signal of the instantaneous shear wave based on the coded excitation technique to obtain a coded ultrasonic echo signal; amplifying and sampling the received coded ultrasonic echo signal to obtain coded detection echo discrete data; decoding according to the digital pulse compression technique to obtain a vibration displacement signal generated during the propagating process of the shear wave in a to-be-detected medium; according to the extracted displacement signal, calculating the speed of the instantaneous shear wave and the elastic modulus of the to-be-detected medium; and outputting the speed of the instantaneous shear wave and the elastic modulus of the to-be-detected medium. According to the technical scheme of the invention, the average emission energy of signals is improved, and the signal-to-noise ratio of echo signals is increased. Meanwhile, the system detection sensitivity and the ultrasonic penetration capability are improved. Moreover, the vibration signal extraction quality of the shear wave is improved, and the depth-detection penetrating power of the instantaneous elasticity imaging process is enhanced.
Owner:SHENZHEN UNIV

Monitoring-video-oriented masked face detection method

The invention relates to a monitoring-video-oriented masked face detection method. The method comprises the steps of: carrying out video frame skipping processing strategies on the basis of simple background difference value operation; realizing moving head and shoulder region detection on the basis of an improved background difference method; and detecting a masked face on the basis of a two-stage Haar classifier. By carrying out the video frame skipping processing strategies on the basis of the simple background difference value operation, the processing range is effectively reduced; the system overhead is reduced; and the processing efficiency is improved. A region with a head and shoulder image is judged to be an effective moving region; the condition that the background is detected to be a moving object by mistake is effectively reduced; by combining the frame skipping strategies, the background is only updated only when the existence of the head and shoulder moving region is detected; and the processing speed can be further accelerated, and the moving target extraction quality of the background difference method can be further improved. According to the Haar linear features of the common masked face, the training and the detection of a masked target are carried out on the basis of a binary image; the gray level distribution rules of training samples and a target to be detected can be intensified and emphasized; and an effect better than that of a pure gray level image is obtained.
Owner:NAT UNIV OF DEFENSE TECH

Surveillance video oriented bearded face detection method

ActiveCN104616006AReduce the chance of being falsely detected as a moving objectGood quality extractCharacter and pattern recognitionPattern recognitionHaar classifier
The invention relates to a surveillance video oriented bearded face detection method. The method mainly used for processing the surveillance videos and comprises the following steps: reading a video file into a computer, decoding, and obtaining a region with human upper body moving targets by using an improved background subtraction method and a human upper body HOG classifier; then, aiming at the upper one third part of the human upper body moving region, detecting the possible face region in the part by adopting a face Haar classifier, and intercepting the lower half part of the face region as a beard detection region; finally, carrying out scale normalization for the region, then calculating the horizontal texture complexity, and judging whether beards exist or not. The method utilizes the motion feature and the morphological feature of the surveillance video oriented bearded faces, can reliably detect the bearded face targets in the walking and running front upright bodies.
Owner:湖南智慧平安科技有限公司

Traditional Chinese medicine extracting and filtering device

The invention discloses a traditional Chinese medicine extracting and filtering device which comprises a reaction kettle and an inner container. The inner container is arranged in an inner cavity of the reaction kettle, a heating cavity is formed between the reaction kettle and the inner container, the top end of one side face of the heating cavity is penetratingly connected with an air incoming pipe, an air shift box is arranged at the top wall of an inner cavity of the inner container, a stirring rod is arranged in the inner cavity of the inner container, the top end of the stirring rod penetrates the top walls of the air shift box and the reaction kettle and is fixedly connected on a motor shaft of a motor, a plurality of stirring pipes are uniformly arranged on the stirring rod, the bottom end of the inner container is penetratingly connected with an agent discharging pipe, the other end of the agent discharging pipe penetrates the bottom wall of the reaction kettle and is penetratingly connected with a filter, and a plurality of supporting legs are arranged at the bottom end of the reaction kettle uniformly. By arranging the heating cavity to wrap the whole inner container to enable the outside of the same to be heated uniformly, lowering of quality of medicine liquid due to charring of traditional Chinese medicine caused by local overheating can be prevented effectively; the medicine liquid is heated from the inside of the same through the stirring rod and the stirring pipes, so that heating effect of the medicine liquid is further improved, and extraction speed and quality of traditional Chinese medicine are improved substantially.
Owner:贵港市鼎金电子产品有限公司

Airflow leaching novel technique and equipment thereof

The present invention discloses an air-stream extraction new process and a device thereof; the new process comprises the steps that a selected solvent is primarily measure and stored in a given stuff-solvent ratio; the solvent is then heated, so that the solvent can be evaporated into air stream at a specified evaporation speed; the solvent air stream is guided into a solid raw material at a normal temperature, so that the solvent air stream is adequately contacted and mixed wit the solid raw material, and the temperature of the solvent air stream decreases, and the solvent air stream is condensed into liquid drops, and the active ingredients are dissolved out to form a mixing solution of the solvent agent and the active ingredients; then the solid substance is separated from the mixing solution containing the active ingredients; and the active ingredients in the solvent mixing solution is extracted, and the solvent is recycled. The device is mainly composed of an upper stream chamber, a material storage chamber, a discharging port, an evaporation chamber, a mixed oil outlet, a solvent inlet, a false bottom, a mixer, a reversed-flow port and a feeding port. The air-stream extraction new process and the device thereof has the advantages such as low energy consumption, high efficiency, simple device, great processing ability, environmental protection, wide application, etc.
Owner:姚建荣

Method for extracting lavender extract by adopting continuous countercurrent tube type ultrasonic waves

The invention provides a method for extracting a lavender extract by adopting continuous countercurrent tube type ultrasonic waves. The method comprises the following steps of: picking stems, leaves and petals of lavender, drying in the shade, and crushing the dried materials into powder with the particle size of 10-40 meshes; inputting the crushed lavender into a continuous countercurrent tube type ultrasonic extractor at a speed of 100kg / h, inputting an organic solvent reversely at a flow speed of 500-1000L / h, maintaining the temperature of an ultrasonic extraction tube to be 25-50 DEG C, and extracting for 20-40 minutes to obtain an extraction mixture of the lavender and the organic solvent; inputting the extraction mixture of the lavender and the organic solvent into a liquid residue squeeze-drying separator, separating residue materials, and performing squeezing separation at a speed of 20-100kg / h to obtain a mixture of the lavender extract and the organic solvent; and inputting the mixture of the lavender extract and the organic solvent into a dynamic loop low-temperature evaporation concentrator, reducing the pressure to -0.08MPa, performing evaporation concentration, maintaining the temperature to be 20-40 DEG C, recovering the organic solvent, and collecting a concentrated extract, thus obtaining the lavender extract, wherein the yield of the lavender extract is more than or equal to 15%.
Owner:伊犁圣玛依拉薰衣草制品有限公司

Production process control system for plant extraction

The invention discloses a production process control system for plant extraction. The production process control system comprises a plant cleaning module, a plant crushing module, a soaking extractionmodule, an impurity separation module and an evaporation concentration module, the plant cleaning module is used for cleaning plants to be extracted, the plant crushing module is used for crushing the cleaned plants, the soaking extraction module is used for extracting effective components from crushed plants to obtain an extracting solution containing the effective components, the impurity separation module is used for removing impurities in the extracting solution, and the evaporation concentration module is used for gasifying and removing part of solvent in the extracting solution. The system has the beneficial effects that a heating control system is arranged in the soaking extraction module of the system, and the PID controller is adopted to control the temperature of the extractingsolution in an extraction tank, so that the temperature is accurately controlled; and the evaporation and concentration module adopts a BP neural network to carry out online detection on the concentration of the extracting solution in an evaporator, so that the real-time detection of the detection concentration is achieved.
Owner:南昌木本医疗科技有限公司

New application of hydroquinone compound

The invention relates to new application of a hydroquinone compound, particularly application of a hydroquinone compound (shikonin) in preparing medicines for resisting hepatitis C virus (HCV). The hydroquinone compound (shikonin) has obvious activity for resisting HCV, and the selection index is up to 43.56; and thus, the hydroquinone compound (shikonin) can be used as a lead compound for treating hepatitis C. The molecular formula of the hydroquinone compound (shikonin) is C16H16O5, and the chemical structural formula is disclosed in the specification.
Owner:KUNMING UNIV OF SCI & TECH

Paeonia lactiflora extract as well as preparation method and application thereof

The invention discloses a peony flower extract as well as a preparation method and application thereof, and belongs to the technical field of plant extraction. The preparation method of the paeonia lactiflora extract comprises the following steps: (1) crushing dried paeonia lactiflora buds, adding an ethanol aqueous solution with the volume ratio of 60%-90% according to the solid-liquid ratio of 1g: (10-30) mL, and leaching at the temperature of 30-60 DEG C to obtain a leaching solution; and (2) removing ethanol in the leaching solution to obtain a concentrated solution, diluting the concentrated solution with water until the pH value is 5-7, and then drying to obtain the peony flower extract. According to the method, main components in the paeonia lactiflora are defined, efficient extraction of four active components in the paeonia lactiflora is achieved, the process is easy and convenient to operate, and the obtained paeonia lactiflora extract is good in quality and stable in quality. The peony flower extract prepared by the invention has the effects of resisting oxidation, resisting aging, blocking sun, whitening, resisting wrinkles and the like, and provides powerful support for the application of the peony flower extract in the aspects of food and cosmetics.
Owner:ZHEJIANG UNIV +1

Cerebroside carnosine injection with good stability as well as preparation method and application of cerebroside carnosine injection

The invention aims to provide a preparation method of a cerebroside carnosine injection with good stability. The preparation method of a rabbit muscle extract for preparing the cerebroside carnosine injection comprises the following steps: mincing pretreated rabbit muscles into small blocks with the volume not higher than 1cm < 3 >; and uniformly mixing the obtained minced muscles with water for injection according to a ratio of 1: (1-3) (w / w), performing homogenizing, freezing the obtained muscle homogenate at a temperature not lower than-10 DEG C for 5-7 days, and performing melting at a temperature of 8-10 DEG C for 36-48 hours. According to the method, the extraction efficiency of the polypeptide in the rabbit muscle extract is remarkably improved, the risk that the rabbit muscle and the rabbit muscle extract are infected by microorganisms is effectively avoided, the freshness of the rabbit muscle extract is remarkably reduced, and the resource utilization rate of the rabbit muscle, the quality of drugs, the quality uniformity, the effectiveness and the safety are remarkably improved.
Owner:BEIJING SIHUAN PHARMA +1

Hickory sterol extractive and preparation method and application thereof

The invention discloses a hickory sterol extractive. The hickory sterol extractive comprises 10 to 50% by weight of total sterol; the total sterol comprises 70 to 95% by weight of beta-sitosterol, fucosterol, campesterol and stigmasterol, wherein the weight ratio of the beta-sitosterol to the fucosterol to the campesterol to the stigmasterol is (70-95): (1-10): (2-12): (1-15). The invention also provides a preparation method of the hickory sterol extractive. The hickory sterol extractive is applicable to the preparation of medicines for reducing blood fat, daily cosmetics, or health care products.
Owner:ZHEJIANG UNIV

Textile plant dye extraction device

The invention belongs to the technical field of textile production, in particular to a textile plant dye extraction device. Directed at the problems that the existing dye extraction device is simple in design structure, cannot extract most of pigments in plants, so that the pigment extraction quality is poor and plants are easily wasted, the invention provides the following scheme that: the devicecomprises a working box, a feeding hole is formed in the outer wall of the top of the working box, a crushing box is inserted into the inner wall of the feeding hole, and a motor is fixed on the outer wall of the top of the crushing box through screws. According to the invention, when cams rotate, under the extrusion action of the cams, bulges and convex particles, juice in plant crushed materials is squeezed out and can fall into a recovery box along filter cloth after being squeezed out, and crushed residues can be left in the filter cloth, so that a good dye extraction effect is achieved,most of pigments in plants can be extracted, the pigment extraction quality is good, and plant waste is not likely to be caused.
Owner:哈尔滨市七彩莲花文化艺术发展有限公司

Natural medical collagen sponge made of sharkskin and a preparation method of the natural medical collagen sponge

The invention discloses a preparation method of a natural medical collagen sponge made of sharkskin and belongs to the technical field of biological medical material preparation. The preparation method includes: removing the blood of the sharkskin, performing quick-freezing at minus 20 DEG C, separating the black skin layer and a collagen layer, removing impurity protein and lipid, and crushing with a crusher; using an acetic acid solution to suspend the processed collagen layer, adding protease, and slowly stirring for enzymolysis to obtain an unmodified collagen solution; separating the obtained collagen solution to obtain supernate and precipitates, subjecting the supernate to salting out, redissolving the precipitates, and using a dialysis membrane to perform dialysis on the redissolved precipitates; subjecting the obtained dialysis solution to crosslinking and freeze drying to obtain a collagen sponge, and packaging to obtain the medical collagen sponge. The preparation method hasthe advantages that aquatic product processing waste such as the sharkskin is sufficiently utilized, the preparation method is simple in production process, high in operability and low in cost, and the prepared collagen sponge is high in purity and widely applicable to the fields such as medical biological materials.
Owner:SHANGHAI HAOHAI BIOLOGICAL TECH

Technology for extracting antibacterial ingredients in propolis

The invention discloses a technology for extracting antibacterial ingredients in propolis, comprising the following steps: taking the propolis raw material, removing impurities, and freezing for 13-15h; adding a crushing medium which accounts for 3-10 wt% of the weight of the propolis raw material into the frozen propolis raw material and crushing, and sieving through a sieve of 40-60 meshes to obtain fine powder; mixing the crushed and screened propolis fine powder with a mixed liquor of water and compound enzyme at a certain ratio, laying the mixed raw materials on a biological enzymolysisbed and carrying out a biological enzymolysis reaction; using ethanol water as a solvent and carrying out microwave extraction on the mixed raw materials obtained after the enzymolysis, filtering, carrying out vacuum concentration on a filtrate to prepare a propolis functional ingredient, and simultaneously recovering the ethanol water while vacuum concentration. Weight ratio of propolis to the ethanol water is 1: 8-15; the microwave treatment time is 2-8 min; and microwave power is 800w-1000w. Problems of existing propolis functional ingredients extraction technologies, such as slow extraction speed and poor purification effect, are solved.
Owner:成都百代禾诗生物科技有限责任公司

Application of Osmanthus fragrans Phenylethyl Glycoside Extract in Preparation of Whitening Cosmetics

The invention discloses application of an osmanthus phenylethanoid glycoside extract in preparing whitening cosmetics. The total phenylethanoid glycoside content in the osmanthus phenylethanoid glycoside extract is 30-90 wt%, and the total phenylethanoid glycosides comprise verbascoside and isoverbascoside. The verbascoside content in the osmanthus phenylethanoid glycoside extract is 25-80 wt%, and the weight ratio of the verbascoside to the isoverbascoside in the total phenylethanoid glycosides is 48:1-300:1.
Owner:ZHEJIANG UNIV

High-efficiency extraction method of peanut leaf DNA

The invention belongs to the technical field of crops, and more specifically relates to a high-efficiency extraction method of peanut leaf DNA. The high-efficiency extraction method comprises following steps: peanut leaf samples are collected; CTAB solution is added into the collected peanut leaf samples in two steps; samples are smashed; the smashed peanut leaf samples are introduced into a waterbath pot for a certain time period of water bath; a chloroform / isoamyl alcohol solution is adopted for a first time of extraction; a phenol / chloroform / isoamyl alcohol solution is adopted for a secondtime of extraction; after extraction, an obtained supernate is collected, precipitation of DNA is carried out, and obtained DNA is washed and stored. The high-efficiency extraction method is capableof realizing high efficiency extraction of peanut leaf DNA at room temperature, and is especially suitable for extraction of DNA of large amounts of samples; extraction cost is relatively; extractiontime is short; batch extraction of a large amount of DNA can be realized; the quality of extracted DNA is basically the same with that of DNA extracted using DNA kits. Operation of the high-efficiencyextraction method is simple, and extraction cost is reduced by half or more of that of extraction using kits.
Owner:INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI

Pharmaceutical composition containing monosialotetrahexosyl ganglioside sodium and application thereof

The invention relates to a pharmaceutical composition containing monosialotetrahexosyl ganglioside sodium, the active ingredients of the composition comprise 0.1-1% (w / w) of monosialotetrahexosyl ganglioside sodium and a rabbit meat extract containing compound amino acid with the concentration of 1-20 mg / ml, the pH value of the composition is 6-8, and the content of the compound amino acid is 1-20 mg / ml. The mass ratio of the monosialotetrahexosyl ganglioside sodium to the compound amino acid in the composition is 1: (1-10). The pharmaceutical composition disclosed by the invention has the beneficial effects of reducing the volume of encephaledema, improving ischemia and hypoxia conditions of a central nervous system, repairing and protecting nerves, remarkably improving the repair rate of pheochromocytoma damaged cells and the like.
Owner:BEIJING SIHUAN PHARMA +1

Image processing method based on near-infrared imaging and terminal

PendingCN110956596AGood human eye recognitionGood quality extractionImage enhancementImage analysisNear infra redHuman eye
The invention discloses an image processing method based on near-infrared imaging and a terminal. The image processing method comprises the steps: respectively obtaining convolution kernels in different directions, the dimension of each convolution kernel being n, and the number of directions being m; obtaining an interested matrix from the original grayscale image in a traversal mode, wherein thedimension of the interested matrix is n; performing convolution operation on the interested matrix and convolution kernels in different directions to obtain m new element matrixes; taking the maximumvalue of the new element values with the same coordinates in the m new element matrixes to obtain a preprocessed image matrix; and performing color assignment on the preprocessed image matrix to obtain a final image. The image processing method performs convolution operation on the original grayscale image and a convolution kernel to obtain a pre-processed image with enhanced blood vessels ratherthan inhibited blood vessels, wherein the pre-processed image still well reserves relative depth information of the blood vessels; and the image processing method performs color assignment on the preprocessed image so that the preprocessed image is enabled to have better human eye identification degree.
Owner:SHENZHEN YUANHUA MEDICAL EQUIP TECH CO LTD

Preparation method and application of mild and non-irritating saponin composition

The invention discloses a preparation method of a mild and non-irritating saponin composition, the preparation method comprises the following steps: (1) sample pretreatment: freezing and crushing Chinese honeylocust peel to obtain Chinese honeylocust peel powder; (2) primary extraction: stirring and centrifuging the Chinese honeylocust fruit tablet powder and distilled water twice to obtain mixed supernate; (3) clarification: adding a flocculant and an adsorption aid into the mixed supernatant, stirring and centrifuging to obtain a precipitate and a clarified solution; (4) decoloring: adding hydroxide, inorganic acid salt, inorganic acid and distilled water into the precipitate, stirring and filtering to obtain filtrate, and mixing the filtrate with the clear liquid; and (5) refining: purifying the clarified liquid through resin, then removing impurities from the clarified liquid by using an electrodialysis device, and then concentrating by using a plate type falling film evaporator to obtain the composition. The method provided by the invention has a good saponin extract extraction effect, and the extracted saponin composition has excellent stability, low irritation and appropriate surface tension, is suitable for popularization in the field of daily chemical products, and has a wide development prospect.
Owner:GUANGDONG JIPURE BIOTECHNOLOGY LTD +1

Zanthoxylum armatum oil production equipment

The invention discloses zanthoxylum armatum oil production equipment which comprises a barrel body, a top cover, a stirring device, a heater and a generator, the barrel body is a pressure barrel; the top cover is hermetically connected with the barrel body; an oil inlet communicated with the interior of the barrel body is formed in the top cover; the heater and the generator are arranged in the top cover through a bracket; and the stirring device and the oil outlet are respectively arranged at the bottom of the barrel body. The zanthoxylum armatum oil is extracted through the device, the whole extraction process is in a closed pressure environment, no pollution is caused, and the treatment energy consumption is low. The zanthoxylum armatum is subjected to dual stimulation of the microwave generator and the ultrasonic generator in the extraction process of the hot oil, the oil temperature is regulated and controlled through the heater, the extraction efficiency is high, and the extraction quality is good. After the zanthoxylum armatum oil is extracted, the residual oil in the barrel is centrifuged to achieve full extraction. After zanthoxylum armatum oil extraction is completed, a pull ring on the inner barrel is buckled through a travelling crane, residues in the inner barrel are cleaned, the next round of production is conducted, design is reasonable, and operation is convenient and fast.
Owner:SICHUAN UNIVERSITY OF SCIENCE AND ENGINEERING

New application of cardiac glycoside compound

The invention discloses an application of a cardiac glycoside compound 12 beta-hydroxycalotropin in preparing medicines for treating and preventing tumors. The cardiac glycoside compound 12 beta-hydroxycalotropin has obvious antitumor activity and can be served as a lead compound for treating the tumors. Moreover, an extraction and separation method of the cardiac glycoside compound 12 beta-hydroxycalotropin provided by the invention is simple and practicable and low in cost, and has high efficiency and yield and good extract quality.
Owner:TROPICAL CORP STRAIN RESOURCE INST CHINESE ACAD OF TROPICAL AGRI SCI

Compound provided with benzogeijerene skeleton and preparation method thereof

InactiveCN102060836BBenzene skeletonHas anti-HCV activityOrganic chemistryMedicineChemical compound
The invention provides a compound provided with a benzogeijerene skeleton and a preparation method thereof. The selection index of the compound on an HCV (Hepatitis C Virus) is 1.75 (or 0.62). The compound is extracted and separated from arnebia euchroma, and the extraction and separation method is simple and easy to implement, has low cost and high efficiency, and ensures high yield and good quality in an extracted substance.
Owner:KUNMING UNIV OF SCI & TECH

Essence purification device containing various types of plant extract

The invention relates to the technical field of essence purification. The invention further discloses an essence purification device containing various types of plant extract. The device comprises a frame, the upper surface of the frame is fixedly connected with a distillation tank, two heating rings are fixedly connected to the outer wall of one side of the distillation tank, a feeding port is formed in one side of the distillation tank, a feeding hopper is fixedly connected to one side of the feeding port, two sliding ways are formed in the inner wall of one side of the feeding hopper, a pushing plate is movably connected into the sliding ways, a tension spring is fixedly connected to one side of the pushing plate, the other end of the tension spring is fixed to the inner wall of one side of the feeding hopper, and an inserting port is formed in one side of the feeding hopper. According to the invention, different materials can be added at different time points for synchronous distillation, the extraction quality of plant essence is better, meanwhile, plant essence gas can be prevented from being emitted, the loss of the plant essence gas is reduced, meanwhile, a protection effect can be achieved on workers, and the workers can rapidly discover abnormalities occurring during distillation.
Owner:耒阳市刘燕酿制生物科技有限公司

A preparation method and application of litchi core extract for preventing and treating prostate cancer

The invention relates to a preparation method and application of litchi seed extract for preventing and treating prostate cancer, and belongs to the technical field of traditional Chinese medicine extracts. The present invention adopts dipping, ultrasonic, microwave, reflux extraction, extraction and other processes to fully destroy the cell wall, accelerate and increase the dissolution of active ingredients, and obtain the litchi core extract of the present invention. The extraction method has high efficiency and good quality of the extract, and can be directly Made into tablets, granules, powders, dripping pills, teas, decoctions, capsules, mixtures or syrups, etc., and added to related foods, beverages or medicines as functional additives for adjuvant therapy or treatment of prostate cancer, Thereby expanding the medicinal scope and value of litchi core.
Owner:广西爱呗利生物科技有限公司

Device and method for extracting total DNA of matrix-attached microorganisms

ActiveCN111269800AOvercoming sampling difficultiesImprove typicality and representativenessBioreactor/fermenter combinationsBiological substance pretreatmentsMicrobial GenomesEngineering
The invention discloses a device and a method for extracting total DNA of matrix-attached microorganisms, and belongs to the technical field of molecular biology, the device comprises a pressure control flushing device, a negative pressure device and a collecting device, the flushing water outlet pressure is controlled through the pressure control flushing device; suspension generated by flushingthe matrix through a flushing pipeline is collected to the collecting device through the negative pressure pipeline; the invention also discloses an extraction method of DNA. The negative pressure device is matched to collect samples; the method overcomes the problems of difficult sampling and incomplete sampling of microbial samples attached to a matrix in the prior art, significantly improves the representativeness, extraction efficiency and quality of microbial genome DNA attached to the matrix, has bright and clear strips, is basically non-degradable, and has good extraction efficiency ondifficult-to-extract microorganisms, such as fungi and the like.
Owner:NANJING UNIV +1

A device and method for extracting the total DNA of substrate-attached microorganisms

The invention discloses a device and method for extracting the total DNA of substrate-attached microorganisms, belonging to the technical field of molecular biology. The device includes a pressure-controlled flushing device, a negative pressure device and a collection device. The pressure of the flushing water is controlled by the pressure-controlled flushing device. The suspension produced by flushing the matrix through the negative pressure pipeline is collected to the collection device. The invention also discloses a DNA extraction method. The matrix is ​​washed under pressure, and the sample is collected with a negative pressure device, which overcomes the prior art. Aiming at the difficulty and incomplete sampling of microbial samples attached to the matrix, the representativeness, extraction efficiency and quality of the microbial genomic DNA attached to the matrix have been significantly improved, the bands are bright and clear, and there is basically no degradation. Fungi, etc. have good extraction efficiency.
Owner:NANJING UNIV +1
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