Natural medical collagen sponge made of sharkskin and a preparation method of the natural medical collagen sponge

A collagen sponge and collagen technology, applied in the field of biomedical material preparation, can solve the problems of shark skin collagen extraction and low thermal denaturation temperature of marine biological collagen, and achieve reliable and safer raw material sources and extraction quality Good, the effect of increasing added value

Inactive Publication Date: 2019-06-21
SHANGHAI HAOHAI BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At this stage, the source of collagen biomaterials is mainly extracted from bovine Achilles tendon and cowhide. Due to problems such as mad cow disease, bird flu, and oral disease, the country strictly controls collagen products derived from animal tissues. Marine biological collagen is due to its function It is excellent, safe, cheap and easy to obtain, so it has broad prospects for development and transformation in the field of biomaterials. However, due to factors such as the low thermal denaturation temperature of marine biological collagen, its practical application is limited, and various methods and technologies are required. Modification treatment can be applied to the production of medical materials
[0004] In recent years, it has been reported that collagen has been prepared from the skin and appendages of grass carp, sturgeon, tilapia, etc., but there is no relevant report on the extraction of collagen from shark skin. Shark cartilage is mainly used to extract chondroitin sulfate, fin rays are used to prepare high value-added products of shark fin, and shark skin is a by-product in the production process, which is usually used as feed processing

Method used

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  • Natural medical collagen sponge made of sharkskin and a preparation method of the natural medical collagen sponge
  • Natural medical collagen sponge made of sharkskin and a preparation method of the natural medical collagen sponge
  • Natural medical collagen sponge made of sharkskin and a preparation method of the natural medical collagen sponge

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preparation example Construction

[0031] The preparation method of the shark skin natural medical collagen sponge provided by the present embodiment comprises the following steps:

[0032] Step 1: Pretreatment of Raw Materials

[0033] Rinse the fresh shark skin with clean water, remove the blood, and freeze it at -22°C to -18°C, separate the black skin layer and the collagen layer of the shark skin, cut the collagen layer into small pieces, and remove the impurities with NaOH , remove lipid with ethanol, then add pre-cooled acetic acid solution, and pulverize with pulverizer;

[0034] Step 2: Enzymolysis

[0035] Suspend the collagen layer treated in step 1 with 0.5M acetic acid solution, add protease at a ratio of 1:1000, and slowly stir and enzymolyze it for 2-3 days at a temperature lower than 10°C to obtain an undenatured collagen solution ;

[0036] Step 3: Purification of the extract

[0037] Place the collagen solution obtained in step 2 in a high-speed centrifuge for high-speed separation; adjust ...

Embodiment 1

[0043] Rinse the skin of the blue shark with clean water to remove excess blood and meat, put it in -20°C for a week of quick freezing, after taking it out, you can easily separate the collagen layer from the black cortex, then take 15g of fish skin, cut it into pieces After forming small pieces, add 450ml of 0.1M NaOH solution, treat for 4hrs, remove impurity proteins, rinse with clean water, add 150ml of 10% ethanol solution, and finally treat for 16hrs, wash with clean water, add 200ml of 0.5M acetic acid solution, After soaking for 20 minutes, crush it with a meat grinder, add 15 mg of pepsin to treat it at a temperature of 10°C, stir and enzymolyze it for 72 hours, remove the undissolved part by centrifugation, adjust the pH of the supernatant to the center with 10M NaOH solution, and then use Salt out with 2.5M NaCl, dissolve the precipitate with 0.5M acetic acid, and dialyze for about 72 hours to obtain undenatured natural shark skin collagen solution, then cross-link wi...

Embodiment 2

[0045]Rinse the skin of the whale shark with clean water to remove excess blood and meat, put it in a -20°C freezer for a week, and then easily separate the collagen layer from the black cortex, then take 20g of fish skin and cut it into pieces After small pieces, add 600ml of 0.1M NaOH solution, treat for 4hrs, remove impurities, rinse with clean water, add 200ml of 10% ethanol solution, and finally, treat for 16hrs, wash with clean water, add 250ml of 0.5M acetic acid solution, soak After 20 minutes, grind it with a meat grinder, add 20 mg of pepsin to treat it at a temperature of 10 ° C, stir for 72 hours, remove the undissolved part by centrifugation, adjust the pH of the supernatant to the center with 10M NaOH solution, and then use 2.5 Salt out with M NaCl, dissolve the precipitate with 0.5M acetic acid, and dialyze for about 72 hours to obtain an undenatured natural shark skin collagen solution, then cross-link with ultraviolet light for 30 minutes, freeze-dry to obtain ...

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Abstract

The invention discloses a preparation method of a natural medical collagen sponge made of sharkskin and belongs to the technical field of biological medical material preparation. The preparation method includes: removing the blood of the sharkskin, performing quick-freezing at minus 20 DEG C, separating the black skin layer and a collagen layer, removing impurity protein and lipid, and crushing with a crusher; using an acetic acid solution to suspend the processed collagen layer, adding protease, and slowly stirring for enzymolysis to obtain an unmodified collagen solution; separating the obtained collagen solution to obtain supernate and precipitates, subjecting the supernate to salting out, redissolving the precipitates, and using a dialysis membrane to perform dialysis on the redissolved precipitates; subjecting the obtained dialysis solution to crosslinking and freeze drying to obtain a collagen sponge, and packaging to obtain the medical collagen sponge. The preparation method hasthe advantages that aquatic product processing waste such as the sharkskin is sufficiently utilized, the preparation method is simple in production process, high in operability and low in cost, and the prepared collagen sponge is high in purity and widely applicable to the fields such as medical biological materials.

Description

technical field [0001] The invention relates to a method for preparing a collagen sponge, in particular to a method for preparing a shark skin natural medical collagen sponge, which belongs to the technical field of biomedical material preparation. Background technique [0002] Collagen is a kind of fibrous structural protein that widely exists in organisms. According to the difference in molecular structure, it is divided into various types such as I, II, and III. Among them, type I collagen is the most important form of existence in organisms. Its structural feature is a triple helical structure formed by two α1 and one α2 peptide chains. Due to the good gel strength, emulsification, low viscosity, biocompatibility, water absorption and moisturizing properties of collagen, it has become popular in recent years. It is widely used in food, medicine, biomaterials, cosmetics and other fields. Among them, the biomedical materials derived from collagen have become one of the fas...

Claims

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Application Information

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IPC IPC(8): A61L24/00A61L24/10C12P21/06C07K14/78C07K1/36C07K1/34C07K1/30
Inventor 于颖肖刚王文斌
Owner SHANGHAI HAOHAI BIOLOGICAL TECH
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