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Technology for extracting antibacterial ingredients in propolis

A technology of antibacterial ingredients and extraction technology, which is applied in the field of extraction technology of antibacterial ingredients in propolis, can solve the problems of poor purification effect and slow extraction speed of functional components of propolis, and achieve slow extraction speed, good extraction quality, and increased extraction volume Effect

Inactive Publication Date: 2018-11-16
成都百代禾诗生物科技有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is that the extraction speed of the existing functional components of propolis is slow and the purification effect is poor. question

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A kind of extraction process of antibacterial component in propolis of the present invention, comprises the following steps:

[0023] S1. Take propolis raw materials, remove impurities, and freeze at -4°C to -10°C for 13 to 15 hours;

[0024] S2, add the crushing medium equivalent to 3-10% of the weight of the propolis raw material to the propolis raw material after freezing in step S1, and pulverize, pass through a 40-60 mesh sieve to obtain a fine powder, and set aside;

[0025] S3. Mix the propolis fine powder crushed and screened in step S2 with a certain ratio of water and compound enzyme mixture, spread the mixed raw materials on the bio-enzymolysis bed, adjust the corresponding temperature, humidity and pH value, and perform bio-enzyme hydrolysis reaction;

[0026] S4, using ethanol aqueous solution as a solvent, microwave extracting the mixed raw materials after enzymolysis, filtering, and vacuum-concentrating the filtrate to obtain propolis functional componen...

Embodiment 2

[0030] Based on Example 1, the weight ratio of propolis to ethanol aqueous solution in step S4 is 1:10, and the concentration of ethanol aqueous solution is 60%.

Embodiment 3

[0032] Based on the above examples, in step S1, the propolis raw material was taken, impurities were removed, and frozen at -8°C for 14.5 hours.

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PUM

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Abstract

The invention discloses a technology for extracting antibacterial ingredients in propolis, comprising the following steps: taking the propolis raw material, removing impurities, and freezing for 13-15h; adding a crushing medium which accounts for 3-10 wt% of the weight of the propolis raw material into the frozen propolis raw material and crushing, and sieving through a sieve of 40-60 meshes to obtain fine powder; mixing the crushed and screened propolis fine powder with a mixed liquor of water and compound enzyme at a certain ratio, laying the mixed raw materials on a biological enzymolysisbed and carrying out a biological enzymolysis reaction; using ethanol water as a solvent and carrying out microwave extraction on the mixed raw materials obtained after the enzymolysis, filtering, carrying out vacuum concentration on a filtrate to prepare a propolis functional ingredient, and simultaneously recovering the ethanol water while vacuum concentration. Weight ratio of propolis to the ethanol water is 1: 8-15; the microwave treatment time is 2-8 min; and microwave power is 800w-1000w. Problems of existing propolis functional ingredients extraction technologies, such as slow extraction speed and poor purification effect, are solved.

Description

technical field [0001] The invention relates to an extraction process, in particular to an extraction process of antibacterial components in propolis. Background technique [0002] Propolis is a colloidal solid with an aromatic smell that is processed by bees from the resin collected from plant spores or tree trunks and mixed with the secretions of their palate glands and wax glands. It is a yellow-brown or dark-brown viscous substance secreted by Apis mellifera and other animals repairing the hive, which can be used as medicine. It is flat in nature, bitter, pungent, and slightly sweet. It has the effects of moisturizing skin and promoting muscle growth, reducing inflammation and relieving pain, and can treat gastric ulcers, oral ulcers, burns, skin cracks, and radiation protection. [0003] The antibacterial effect of propolis is due to the compounds such as flavonoids, aromatic acids and their esters contained therein. Galangin, pine needles and pine needles are the fla...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/644A61P31/04
CPCA61K35/644A61P31/04
Inventor 宋立波杜烨刘金凤熊丹梅张亮富杜琳黄清东
Owner 成都百代禾诗生物科技有限责任公司
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