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Mineralized collagen-based biological tooth root scaffold material and preparation method thereof

A technology of mineralized collagen and scaffold materials, applied in medical science, prostheses, etc., can solve the problems of maintenance, slow degradation of calcium phosphate bioceramics, unfavorable reconstruction of biological tooth roots, etc., and achieve the effect of broad clinical application prospects

Inactive Publication Date: 2016-02-17
CAPITAL UNIVERSITY OF MEDICAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this calcium phosphate bioceramic has major disadvantages: 1. Calcium phosphate bioceramic is only an inorganic ceramic material with a porous or dense structure, and does not have the bionic structure and chemical composition of the tooth root, which is not conducive to the reconstruction of biological tooth roots; 2. Calcium phosphate bioceramics are relatively brittle, which is not conducive to maintaining the integrity of the scaffold during and after surgery; 3. The degradation of calcium phosphate bioceramics is slow, which is not conducive to the reconstruction of biological tooth roots in a short period of time (3 to 6 months)
[0009] Therefore, in order to realize the successful construction of biological tooth roots in clinical practice, it is necessary to provide a material and scaffold with a biomimetic structure and chemical composition of the tooth root, as well as suitable mechanical properties and biodegradable properties, while the existing biomedical materials and scaffolds are not yet available. With these characteristics, it is not suitable for the construction of biological roots

Method used

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  • Mineralized collagen-based biological tooth root scaffold material and preparation method thereof
  • Mineralized collagen-based biological tooth root scaffold material and preparation method thereof
  • Mineralized collagen-based biological tooth root scaffold material and preparation method thereof

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preparation example Construction

[0055] figure 1 It is a flow chart of the method for preparing the mineralized collagen-based biological tooth root material and the biological tooth root bracket of the present invention. According to the flowchart, the preparation steps of the mineralized collagen-based biological tooth root material and the biological tooth root bracket of the present invention include:

[0056] Step S1, preparation steps of biological tooth root material:

[0057] Step S1-1, dissolving collagen in any one of hydrochloric acid, nitric acid or acetic acid to prepare an acid solution of collagen, wherein the concentration of collagen is 5.0×10 -5 ~5.0×10 -3 g / mL;

[0058] Step S1-2, continuously stirring the solution obtained in step S1-1, and slowly adding the solution containing calcium ions dropwise, the amount of calcium ions added is 0.01-0.16 mol of calcium ions per gram of collagen;

[0059] Step S1-3, continuously stirring the solution obtained in step S1-2, slowly adding the solu...

Embodiment 1

[0079] Example 1: Preparation of a mineralized collagen-based biological tooth root scaffold material with an HA content of 70 wt%.

[0080] Step S1-1, dissolving collagen in 0.5wt% acetic acid solution to prepare an acid solution of collagen, wherein the concentration of collagen is 5.0×10 -4 g / mL;

[0081] Step S1-2, continuously stirring the solution obtained in step S1-1, slowly adding the solution containing calcium ions dropwise, the amount of calcium ions added is 0.05 mol of calcium ions per gram of collagen, and part of the calcium ions are free in the solution, not Can be adsorbed on collagen fibers;

[0082] Step S1-3, continuously stirring the solution obtained in step S1-2, slowly adding the solution containing phosphate ions dropwise, the molar ratio of the amount of phosphate ions added to the amount of calcium ions added in step S1-2 is Ca / P=5 / 3;

[0083] Step S1-4, continue to stir the solution obtained in step S1-3, slowly add NaOH solution dropwise to th...

Embodiment 2

[0092] Example 2: Preparation of mineralized collagen-based biological root scaffold material with HA content of 70 wt % and PCL content of 10 wt %, wherein collagen / apatite=2.2 / 7.8 (w / w).

[0093] Steps S1-1 to S1-5 are basically the same as steps S1-1 to S1-5 of Example 1, except that in step S1-2, the amount of calcium ions added is 0.075 mol of calcium ions per gram of collagen.

[0094] Step S1-6, freeze-dry the jelly obtained in step S1-5, grind and obtain mineralized collagen powder.

[0095] Step S1-7, the PCL is prepared into a solution with a mass volume concentration of 0.05 g / mL at 50° C., and the solvent is 1,4-dioxane;

[0096] Step S1-8, adding the mineralized collagen powder obtained in step S1-6 to the PCL solution obtained in step S1-7, and mixing evenly to obtain a mixed suspension of mineralized collagen / PCL, wherein mineralized collagen / PCL=9 / 1(w / w);

[0097] Step S2b-1, detecting the content of solid matter in the mineralized collagen / PCL mixed suspens...

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Abstract

The invention provides a biological root scaffold. The biological root scaffold is prepared from animal-origin collagen I and hydroxyapatite, has certain pore structure in microscopic scale, has porosity 70%-95%, pore diameter 50-500 MuMu and has a macroscopically cylindrical shape, cylinder height is 9-12 mm; the shape of the outer side of a cylinder truncated by an axial plane perpendicular to the cylinder is circular or approximately circular; the scaffold is 11-14 mm in perimeter. The mineralized collagen-based biological root scaffold material and a biological root scaffold can be obtained by implementing the invention. The scaffold material has chemical composition and microscopic structure consistent with dentin of a natural tooth root, the shape of the scaffold is close to that of the natural tooth root, the micrometer porous structure and macroscopic internal structure of the scaffold help carry cells, growth factors, pharmaceuticals and the like for the ingrowth of the cells, and therefore the biological root scaffold has ingredients and structure consistent with those of the natural tooth root, is good for the construction of biological tooth roots in root regeneration application and has a promising clinical application prospect.

Description

technical field [0001] The invention relates to the field of biomedical materials, in particular to a mineralized collagen-based biological tooth root support material and a preparation method thereof. technical background [0002] Tooth loss caused by various reasons has always plagued human physical and mental health, and the restoration methods of missing teeth have also changed from simple and rough to complex and sophisticated along with the progress of human beings. Nevertheless, currently commonly used restoration methods such as removable dentures, fixed dentures, implants These are all mechanical repairs, with various defects, lack of real physiological repairs, and cannot meet the needs of human beings. Therefore, how to use artificial methods to reconstruct biologically active teeth to repair missing teeth and realize tooth regeneration is one of the hot topics that scholars at home and abroad focus on. [0003] From the development of tooth regeneration research...

Claims

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Application Information

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IPC IPC(8): A61L27/24A61L27/12A61L27/58A61L27/50
Inventor 王秀梅裘志烨王硕崔福斋王松灵胡磊韩子韵高振华
Owner CAPITAL UNIVERSITY OF MEDICAL SCIENCES
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