Biologically active native biomatrix composition

Abstract

The present disclosure relates to bioactive biomatrix compositions. In one embodiment, the biomatrix composition is derived from human amniotic membrane. This biomatrix is ​​called HuBiogelTM. The HuBiogel™ composition precisely mimics the composition of naturally occurring basement membranes and is capable of supporting a variety of cell types in vitro and in vivo. The disclosed HuBiogel™ biomatrix contains laminin, collagen I, and collagen IV in one embodiment, and may further contain any combination of the following: nestin, tenascin, fibronectin, and proteoglycans . The biomatrix composition is substantially free of endogenous growth factors and proteolytic enzymes. The present invention also describes two-dimensional and three-dimensional culture systems and physiological / pathological model systems using HuBiogel™ compositions. HuBiogel(TM) compositions may be modified to contain desired growth stimulators, such as growth factors, polypeptides and small organic molecules, and may also contain growth inhibitory and / or therapeutic agents.

Description

technical field [0001] The present disclosure relates to a bioactive biomatrix composition derived from human amniotic membrane (called HuBiogel TM ). Background technique [0002] The basement membrane (BM) is a continuous structural barrier that separates epithelial tissue from the adjacent stroma. The major components of BM include laminin, collagen types I and IV, nestin, tenascin, and proteoglycans, such as heparan sulfate proteoglycans. Because cells constantly interact with their extracellular environment, BM is involved in many in vivo processes. These processes include physiological processes such as tissue growth and development, bone remodeling, angiogenesis, reproduction, wound healing, and neuronal regeneration, as well as pathological processes such as tumorigenesis / metastasis (including cell migration, invasion, and angiogenesis), angiogenesis Dysfunction, arthritis and aging, and atherosclerosis. During each of these processes, cells interact with and res...

AI Technical Summary

Problems solved by technology

[0005] Modifications of the original Matrigel formulations have been attempted, including Matrigel supplemented with collagen and Matrigel containing smaller amounts of growth factors, but even these formulations do not provide reliably reproducible in vitro assays for a variety of physiological and pathological processes. BM system

The use of Matrigel has been hampered by the limitations of its physical properties

For example, the physical form of Matrigel (i.e., gel or liquid) is sensitive to temperature, which can create certain limitations for use in in vitro assays

In addition, it should be noted that Matrigel is derived from mouse BM fractions, raising the possibility that studies using this fraction in human model systems could produce artifacts

[0006] The prior art lacks defined, bioactive biological matrices derived from human components

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