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Tussah silk fibroin film and preparation method thereof

A technology of tussah silk fibroin and tussah silk, which is applied in the field of natural polymer medical biomaterials, can solve the problems of tussah silk film hardness, poor mechanical properties, and affecting biocompatibility, and achieve good biocompatibility, softness good sex effect

Active Publication Date: 2011-07-27
苏州盛泽科技创业园发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the chemical treatment reagents for tussah silk film mainly include methanol and ethanol, but the tussah silk film treated with methanol or ethanol is hard and brittle, and its mechanical properties are poor.
In order to improve these properties, some literatures have reported a technical scheme of chemically crosslinking tussah silk membranes using polyethylene glycol glycidyl ether as a crosslinking agent. This method can significantly improve the water resistance and toughness of the membrane ([J] China Journal of Nonferrous Metals, 2004, 14(6), 414-419), but the crosslinking agent used is epoxy compound, which remains in the silk film, which will affect the biocompatibility of the prepared material

Method used

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  • Tussah silk fibroin film and preparation method thereof
  • Tussah silk fibroin film and preparation method thereof
  • Tussah silk fibroin film and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] The preparation steps of the water-insoluble tussah silk fibroin film provided by the present embodiment are as follows:

[0015] 1. Put 0.1 kg of tussah raw silk into 3 liters of 0.15% sodium carbonate aqueous solution, and treat it at 98-100°C for 2 hours to degumming the raw silk, and get pure silk fibroin (ie tussah silk fibroin) after fully washing. The dried pure silk fibroin is heated and dissolved at 60±5° C. with 1 liter of lithium thiocyanate solution with a concentration of 15 mol / liter to obtain a tussah silk fibroin protein mixed solution.

[0016] 2. Using a cellulose membrane as a dialysis material, dialyze the obtained tussah silk fibroin protein mixed solution with deionized water to remove impurities such as lithium thiocyanate to obtain a tussah silk fibroin protein solution with a mass concentration of 10%.

[0017] 3. Add ethylene glycol to the above tussah silk fibroin protein solution. According to the mass ratio, the amount of ethylene glycol add...

Embodiment 2

[0024] Put 0.5 kg of tussah raw silk into 25 liters of neutral soap solution with a mass concentration of 0.5%, treat it at 98-100°C for 2 hours to degumming, and get pure silk fibroin (ie tussah silk fibroin) after fully washing. The dried pure silk fibroin is dissolved with 2 liters of 16 mol / liter lithium thiocyanate aqueous solution and stirred at 60±2° C. to form a tussah silk fibroin protein mixed solution.

[0025] Using cellulose membrane as the dialysis material, the obtained tussah silk fibroin protein mixed solution was dialyzed with deionized water to remove impurities such as lithium thiocyanate to obtain a tussah silk fibroin protein solution with a mass concentration of 20%.

[0026] 1,3-propanediol is added to the pure tussah silk fibroin protein solution, and the amount of 1,3-propanediol added (according to mass ratio) is 45% of the solid content in the tussah silk fibroin protein solution.

[0027] The silk fibroin solution is injected into the mold to form ...

Embodiment 3

[0033] Put 200 grams of tussah raw silk into 8 liters of sodium carbonate aqueous solution with a mass concentration of 0.05%, add sodium lauryl sulfate with a mass concentration of 0.01%, and treat it at 98-100°C for 0.5 hours to degumming, repeat the treatment 3 times, and fully Pure silk fibroin is obtained after washing. The dried pure silk fibroin is heated and dissolved in 1 liter of 14 mol / liter lithium thiocyanate aqueous solution at 65±5° C. to obtain a tussah silk fibroin protein mixed solution.

[0034] Using cellulose membrane as the dialysis material, the obtained tussah silk fibroin protein mixed solution was dialyzed with deionized water for 3 days to obtain a tussah silk fibroin protein solution with a mass concentration of 16%.

[0035] Add 1,2-butanediol to the pure tussah silk fibroin protein solution, and the added amount of 1,-2 butanediol (by mass ratio) is 50% of the solid content in the tussah silk fibroin protein solution.

[0036] The silk fibroin so...

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Abstract

The invention relates to technology of medical biological material with natural polymer, and discloses a tussah silk fibroin film and a preparation method thereof. Nontoxic dihydric alcohol is added into tussah silk solution so as to be taken as a cross-linking agent which is injected into a mold, and the tussah silk fibroin film is formed after the mixture drying. According to weight, the tussah silk fibroin film contains 40-90% of tussah silk fibroin; the protein leaching loss of the film in water with the temperature 37 DEG C for 24 hours is less than 3%, the tensile strength at break is greater than 20Mpa, and the tensile elongation at the break is greater than 20%. As an extra chemical cross-linking agent or chemical denaturant does not need to be added in the preparation process, the biocompatibility of the tussah silk fibroin can not be reduced, and can be kept good. The tussah silk fibroin difficult to dissolve can be used for covering materials for artificial cornea, endocranium, gullet, heart valve, blood vessels and wound surfaces, and can be used for temporal covering, and isolating tissues, and can be taken as medicine releasing materials and the like.

Description

technical field [0001] The invention relates to a natural polymer medical biomaterial technology, in particular to a tussah silk fibroin protein film and its preparation method. The prepared material can be applied to artificial cornea, dura mater, esophagus, heart valve, blood vessel and wound surface covering material Wait. technical background [0002] Tussah silkworm is a kind of wild silkworm that feeds on tussah leaves. Tussah silk is a natural silk fiber reeled from tussah cocoons. my country produces 30,000 to 40,000 tons of tussah cocoons annually, accounting for more than 90% of the world's total output of tussah cocoons. This is a rare resource in the world and unique to my country. Very limited value. The protein composition of tussah silk fibroin is mainly composed of glycine, alanine and serine, which has good biocompatibility, itself and its degradation products are non-toxic to cells and the body, and will not or less cause inflammation and immune rejection...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/22A61L15/32
Inventor 卢神州李明忠毛丽
Owner 苏州盛泽科技创业园发展有限公司
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